Development and optimization of a HPLC-RI method for the determination of major sugars in apple juice and evaluation of the effect of the ripening stage

Zielinski, Acácio Antônio Ferreira; Braga, Cíntia Maia; Demiate, Ivo Mottin; Beltrame, Flávio Luís; Nogueira, Alessandro; Wosiacki, Gilvan

doi:10.1590/s0101-20612014005000003

Cited by 49 publications

(24 citation statements)

References 24 publications

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“…Sugar profiles were determined by the method described [ 18 ] high performance with modification that liquid chromatography coupled to a refraction index detector (HPLC-RI). Soluble sugar determined at 80 °C.…”

Section: Methodsmentioning

confidence: 99%

Antiosteoporotic effect of Petroselinum crispum, Ocimum basilicum and Cichorium intybus L. in glucocorticoid-induced osteoporosis in rats

Hozayen

El-Desouky

Soliman

et al. 2016

BMC Complement Altern Med

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BackgroundGlucocorticoid-induced osteoporosis (GIO) is one of the serious side effects which have become the most common secondary osteoporosis. The purpose of this study is to evaluate the effect of aqueous extract of parsley, basil and chicory on glucocorticoid-induced osteoporosis in rats.MethodsFifty Female rats were divided into five groups and treated for 8 weeks as follow: group 1 served as control; group (2) subcutaneously injected with 0.1 mg/kg b. wt. dexamethasone dissolved in saline; group 3 received similar dose of dexamethasone together with aqueous parsley extract in a dose of 2 g/kg b. wt.; group 4 received similar dose of dexamethasone together with 400 mg/kg b. wt. aqueous basil extract and group 5 received similar dose of dexamethasone together with 100 mg/kg b. wt. aqueous chicory extract.ResultsThe dexamethasone group showed a significant decrease in serum E2, Ca, P levels and significant decrease in total BMD, BMC and a significant increase in serum PTH, ALP and ACP. Bone TBARs was significantly increased while GSH, antioxidant enzymes were significantly decreased. These changes were attenuated by parsley, basil and chicory extracts in the group 3, 4 and 5 respectively.ConclusionAqueous extracts of parsley, basil and chicory showed bone protection against glucocorticoid-induced in rats. From our results, we concluded that chicory has a potent protective effect more than parsley and basil due to containing flavonoids and inulin.

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Section: Methodsmentioning

confidence: 99%

Antiosteoporotic effect of Petroselinum crispum, Ocimum basilicum and Cichorium intybus L. in glucocorticoid-induced osteoporosis in rats

Hozayen

El-Desouky

Soliman

et al. 2016

BMC Complement Altern Med

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“…Flavonoid, phenolic and polysaccharides in the ethanolic extract of Dictyota dichotoma were determined according to method described by Goupy et al (1999) and Zielinski et al (2014). The detection were conducted using high performance liquid chromatography (HPLC Agilent 1200 series) equipped with Quaternary pump, Auto sampler, column compartment set at 35°C, multi wavelength detector set at 330 nm, 280 nm.…”

Section: Chemical Analysis Of Dictyota Dichotoma Hplc Analyses Of Flamentioning

confidence: 99%

Impact of The Microbial Suppression by Using The Brown Alga Dictyota dichotoma Extract

et al. 2017

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I N THIS CONTEXT the brown alga Dictyota dichotoma isolated from Hurghada SeaShore , Red-Sea coast, Egypt (June 2015) was screened for its antimicrobial bioactivity. A laboratory experiment was conducted to test activity of ethanol extract of the brown alga Dictyota dichotoma against three types of Gram-positive and six of Gram-negative bacteria, and one type of fungi. Ethanol extract was selected for this study by using different concentrations (100, 200, 300, 400 mg) of alga extracts. Result revealed that the alga ethanolic extract exhibited high suppression activity against all tested pathogens. HPLC analysis of the chemical composition of algal extract was detect the presence of high amount of certain ingredients such as Flavonoids

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“…HPLC analysis of sugars content of persimmon and husk tomato were done according to the method of Zielinski et al(2014).…”

Section: 23-sugars Contentmentioning

confidence: 99%

Nutrient and Phytochemical Compounds of Persimmon and Husk Tomato

2017

AJAS

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The aim of this study was to determine the proximate chemical composition, minerals content, some vitamins, sugars composition, flavonoids content as well as phenols content of whole persimmon and husk tomato fruits. The results indicated that, husk tomato has higher amounts of crude protein, crude fat, crude fiber and ash compared with persimmon fruits. Iron (Fe) was the most predominant of micro-elements found in persimmon and husk tomato. Persimmon fruits contained much more Vit. K content compared with husk tomato fruits. Glucose, arabinose, xylose, glactose and fructose were the predominant sugars of both of persimmon and husk tomato fruits. The major flavonoids of persimmon fruits were narengin, luteolin, hesperidin, quercetrin and rosmarinic acid, while it were rosmarinic acid, luteolin, rutin, querectin and narengin in husk tomato fruits.Regarding to phenolic compounds, pyrogallol was the major phenolics in both of persimmon and husk tomato followed by catechen in persimmon and benzoic acid in husk tomato. Keywords: Persimmon, husk tomato, phenols, flavonoids, vitamins. 1-IntroductionThe health promoting potentials associated with their consumption are mainly due to presence of bioactive components and these phytochemicals are distinct bioactive molecules widely acknowledged for their beneficial roles in human physiology (Manach et al., 2004).Phytochemicals are biologically active, naturally occurring chemical composition found in fruits, vegetables, grains, nuts, tea and seeds that promote human health and prevent diseases. The abundance of scientific evidence indicates that such bioactive compounds have biological properties such as antioxidant activity, antimicrobial effect, modulation of detoxification enzymes, stimulation of the immune system, decrease of platelet aggregation and modulation of hormone metabolism and anticancer property (Wavinya et al., 2016).Phytochemicals are not essential nutrient and are not required by the human body for sustaining life, but have important properties to prevent or to fight some common diseases (Holst and Williamson, 2008).Persimmon (Diospyros Kaki L.) is fleshy fibrous tropical, deciduous fruit belonging to Ebenceal family. It is commonly cultivated in warm regions of the world including China, Korea, Japan, Brazil, Turkey and Italy (Itamura et al., 2005 ;Yokozawa et al., 2007). Mediterranean region is also suitable for persimmon production (Luo, 2007;Bubba et al., 2009). Persimmon is prominent for its nutrition comprising about 80.3% water, 0.58% protein, 0.19% total lipids, 18.6% total carbohydrates and up to 1.48 g and 7.5 mg total dietary fiber Rashwan, et al., 2017 http://ajas.js.iknito.com/ 103 and ascorbic acid, respectively (Achiwa et al., 1997;Ozen et al., 2004 andErcisli et al., 2007). Persimmon is naturally bestowed with bioactive molecules including proanthocyanidins, flavonoids, tannins, phenolis, carotenoids and dietary fiber (Butt et al., 2015).Husk tomato fruit (Physalis pubescens L.) belongs to the Solanaceae family. It is native ...

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Development and optimization of a HPLC-RI method for the determination of major sugars in apple juice and evaluation of the effect of the ripening stage

Cited by 49 publications

References 24 publications

Antiosteoporotic effect of Petroselinum crispum, Ocimum basilicum and Cichorium intybus L. in glucocorticoid-induced osteoporosis in rats

Antiosteoporotic effect of Petroselinum crispum, Ocimum basilicum and Cichorium intybus L. in glucocorticoid-induced osteoporosis in rats

Impact of The Microbial Suppression by Using The Brown Alga Dictyota dichotoma Extract

Nutrient and Phytochemical Compounds of Persimmon and Husk Tomato

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