Development and Initial Testing of a Model for in vitro Formation of Pit and Fissure Caries (Short Communication)

Katz, Simon; Park, K.K.; Stookey, George K.; Schemehorn, B.R.

doi:10.1159/000260968

Cited by 15 publications

(3 citation statements)

References 5 publications

(5 reference statements)

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“…Fifty milligrams of hydroxyapatite beads (particle size of 200 µm; Tonen, Tokyo, Japan) were washed with 2.0 ml of Katz's artificial saliva: 0.3 mM CaSO 4 , 1.0 mM NaCl, 0.7 mM KCl, 0.4 mM KH 2 PO 4 and 0.4 mM Na 2 HPO 4 (pH 6.8) [Katz et al, 1986], thereafter centrifuged at 800 g for 5 min. This washing treatment was repeated twice.…”

Section: Adsorption Of Chlorhexidine To Hydroxyapatitementioning

confidence: 99%

High–Performance Liquid Chromatographic Analysis of Chlorhexidine in Saliva after Mouthrinsing

Tsuchiya¹,

Miyazaki²,

Ohmoto³

1999

Caries Res

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A high–performance liquid chromatographic method was developed to quantify chlorhexidine in human saliva. After addition of an internal standard and acidicdeproteinization, saliva samples were chromatographed by the reversed–phase ion–pair system using pentanesulfonate as a counterion and the eluates were detected by a diode array detector. Under optimal conditions, the baseline separation of analytes was achieved within 4.5min without interference from components in saliva matrix. The method showed high selectivity to salivary chlorhexidine, quantitative range (50.0ng/ml–50.0μg/ml), recovery (96.0–97.8%) and analytical precision (intra– and interassay CVs within 0.41%), permitting the effective application to both saliva and aqueous solutions. Chlorhexidine was found in saliva at microgram per milliliter levels for at least 8 h after mouthrinsing with 10 ml of an aqueous solution of chlorhexidine (0.1 or 1.0 mg/ml) for 1 min. The concentrations of salivary chlorhexidine were reduced by ingesting the acidic beverage and food, but not by ingesting the neutral beverage. The adsorption experiments in artificial saliva revealed that chlorhexidine was significantly adsorbed to saliva–coated hydroxyapatite, buccal epithelial cells and mucin. The proposed method will be a useful tool to study salivary chlorhexidine after oral application and its retention in the oral cavity.

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Section: Adsorption Of Chlorhexidine To Hydroxyapatitementioning

confidence: 99%

High–Performance Liquid Chromatographic Analysis of Chlorhexidine in Saliva after Mouthrinsing

Tsuchiya¹,

Miyazaki²,

Ohmoto³

1999

Caries Res

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“…Since the oral environment is difficult to control and varies with location, over time, and between persons (Scheie et al, 1992), and because human and animal studies are restricted by ethical issues and expense (Zero, 1995), in vitro systems have been developed to study dental caries. Two basic methods exist at present for providing an artificial cariogenic challenge to the tooth structure: purely chemical systems (Kidd, 1976;Grieve, 1978;Featherstone et al, 1986;Silverstone et al, 1988), which use an acidic environment to demineralize teeth; and bacterial systems, in which a specific bacterial culture is used to induce demineralization (Donoghue et al, 1985;Katz et al, 1986;Noorda et al, 1986a,b;Dérand et al, 1991;Gilmour et al, 1993;Fontana et al, 1996a;Deng & ten Cate, 2004). Two basic methods exist at present for providing an artificial cariogenic challenge to the tooth structure: purely chemical systems (Kidd, 1976;Grieve, 1978;Featherstone et al, 1986;Silverstone et al, 1988), which use an acidic environment to demineralize teeth; and bacterial systems, in which a specific bacterial culture is used to induce demineralization (Donoghue et al, 1985;Katz et al, 1986;Noorda et al, 1986a,b;Dérand et al, 1991;Gilmour et al, 1993;Fontana et al, 1996a;Deng & ten Cate, 2004).…”

Section: Introductionmentioning

confidence: 99%

Caries lesion development and biofilm composition responses to varying demineralization times and sucrose exposures

2004

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Link to this article: http://journals.cambridge.org/abstract_S1479050504001474How to cite this article: M. Fontana, A. Haider and C. González-Cabezas (2004). Caries lesion development and biolm composition responses to varying demineralization times and sucrose exposures. A B S T R A C TThe aim of this research was to study the effect of varying incubation times and sucrose exposures on lesion development and biofilm composition using a multi-species biofilm caries model. Two studies were conducted. In study 1, enamel specimens were divided into four groups, inoculated with a mixed overnight culture of Streptococcus mutans, Lactobacillus casei, Actinomyces naeslundii, Streptococcus parasanguis and Streptococcus salivarius, and exposed to circulating trypticase soy broth + 5% (w/v) sucrose (TSBS; 30 min, three times per day (3×/day)) and a mineral washing solution containing 0.25 p.p.m. fluoride (22.5 h/day) for 2, 5, 6 or 8 days. In study 2, additional enamel specimens were divided into four groups and exposed to the same biofilm for 7 days, but with variations in the feeding schedule: TSBS 3×/day for 5 min, TSBS 3×/day for 15 min, TSBS 3×/day for 30 min, or TSBS 3×/day for 30 min + 1×/day for 15 min. At the end of each study, bacterial colonization counts and lesion size were determined. In study 1, specimens developed significantly deeper carious lesions with longer demineralization time (average lesion depth was 52.16 µm, 67.86 µm, 84.91 µm, and 99.97 µm, respectively, for 2, 5, 6, and 8 days). In study 2, there was no significant difference in size between the lesions developed at feeding schedules of 3×/day for 5 or 15 min. Lesions exposed to longer (30 min) and more frequent feeding schedules (4×/day) were significantly larger than the other groups. In both studies, all five bacterial species were able to colonize the enamel and were present in all groups at the end of the experiments, with predominance of lactobacilli over S. mutans. In conclusion, larger lesions were observed with increased incubation time and more frequent feeding schedules, with small variations in biofilm composition.

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“…4 Artificial saliva: Artificial saliva prepared using the method of Katz was used for in vitro study. 15 Durometer: A durometer, which has been reported to be an appropriate tool for measurement of softness, was used to evaluate the softness of the tissue conditioner (Fig. 3).…”

Section: Methodsmentioning

confidence: 99%

A study to evaluate the effect of monopoly on the softness of soft liner by durometer

Singh

Tripathi

Chand

et al. 2013

Indian Journal of Dentistry

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Resin disksDurometer Monopoly a b s t r a c t Objectives: This study was designed to examine the effect of monopoly on the softness of a soft liner at various time intervals to save cost and time during replacement of liner. Methods: A total of eighty specimens were prepared and divided into two equal groups.Group I (control group) of 40 specimens were lined with soft liner and left uncoated. Group II (test group) of 40 specimens were lined with soft liner and coated with a monopoly. The 10 specimens each of both groups were then examined for softness with a durometer at the baseline, and after 2, 4 and 8 weeks. Results: Softness on the American Standards for Testing Materials scale showed a significant ( p < 0.001) difference between the control and test groups. Conclusion: Within the limitations of this study, our data suggest that application of a monopoly can significantly reduce the loss of softness of a soft liner.

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Development and Initial Testing of a Model for in vitro Formation of Pit and Fissure Caries (Short Communication)

Cited by 15 publications

References 5 publications

High–Performance Liquid Chromatographic Analysis of Chlorhexidine in Saliva after Mouthrinsing

High–Performance Liquid Chromatographic Analysis of Chlorhexidine in Saliva after Mouthrinsing

Caries lesion development and biofilm composition responses to varying demineralization times and sucrose exposures

A study to evaluate the effect of monopoly on the softness of soft liner by durometer

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