Development and in�vitro evaluation of mucoadhesive patches of methotrexate for targeted delivery in oral cancer

Abstract

Abstract. The present study focused on the development of a mucoadhesive patch of methotrexate (MTX) for targeted delivery in oral cancer. Initially, MTX-loaded liposomes were prepared using the thin film hydration method, and had a mean diameter of 105.7-137.4 nm and percentage entrapment efficiency of 54.6±3.5. These liposomes were cast in optimized mucoadhesive film. The film was characterized by its release pattern, thickness, weight and percentage swelling index and the sustained release profile of the optimized film was evaluated. The developed liposomes and liposomes cast in the film formulation were evaluated for cytotoxicity in HSC-3 cells using an MTT assay, and a significant decrease in the half maximal inhibitory concentration of MTX was identified with the MTX-entrapped liposomal film, M-LP-F7. The results of the mitochondria-dependent intrinsic pathway demonstrated that there was significant mitochondrial membrane potential disruption with M-LP-F7 compared with the plain drug. M-LP-F7 increased the rate of apoptosis in HSC-3 cells by almost 3-fold. Elevated levels of reactive oxygen species provided evidence that M-LP-F7 exerts a pro-oxidant effect in HSC-3 cells.

Enhancing Stability and Tooth Bleaching Activity of Carbamide Peroxide by Electrospun Nanofibrous Film

“…Nanofibers with high adhesive property can provide prolonged residence time on the desired site of action and thereby exert a better bleaching effect. Due to the good adhesive properties of PVA and PVP, previous studies have used these polymers in mucoadhesive dosage forms [ 40 , 41 ]. Among the seven samples, ENF from PP-CP possesses the highest adhesive force with a value of 0.81 ± 0.02 N followed by PPS-CP3, PPS-CP1, PPS-CP2, P-CP, and PS-CP, respectively ( Table 3 ).…”

Nanoparticles in Dentistry: A Comprehensive Review

“…A wide variety of NPs were proven to be effective against a number of oral cancer cell lines, including human Caucasian dysplastic oral keratinocytes (DOK) [ 205 , 207 ], mouth epidermoid carcinoma (KB) [ 203 , 208 , 209 , 210 , 211 , 212 ], murine AT-84 oral squamous carcinoma cells [ 213 ], oral squamous cancer cell lines of Asian origin (ORL-48 and ORL-115) [ 214 ], human oral squamous cell carcinoma lines (PE/CA-PJ15 [ 215 ], OEC-M1 [ 216 ], HSC2 [ 217 ], YD-9 [ 218 ], SAS [ 219 , 220 ], HSC4 [ 1 , 220 ], KOSC [ 220 ], HSC3 [ 204 , 221 , 222 ], HSC-3-M3 [ 7 ], Ca9-22 [ 223 ], CAL 27 [ 222 , 223 , 224 , 225 , 226 , 227 ], SCC131 [ 228 ], SCC4 [ 222 , 228 ], VB6 [ 229 ], and H357 [ 229 , 230 , 231 ]), human tongue carcinoma cell lines (SCC-9 [ 232 , 233 , 234 ], SCC-25 [ 224 , 230 , 233 , 235 ], SCC-15 […”

Buccal Film: A Novel Approach for Oral Mucosal Drug Delivery System

“…By acquiring nano delivery systems in the form of films consisting of polymers, it has been concluded an increase in solubility, stability, and bioavailability accumulated even inside tumor cells. A study was conducted for the development of liposomes loaded in the film and was evaluated for its cytotoxicity in hematopoietic stem cells (HSC-3) [92].…”