Development and Field Validation of a Beta-cyfluthrin-Based ‘Attract-and-Kill’ Device for Suppression of Asian Citrus Psyllid (Hemiptera: Liviidae) on Residential Citrus
Abstract:An ‘attract-and-kill’ (AK) device was evaluated for suppression of adult Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), on residential citrus. The AK device, made from weather-resistant plasticized PVC, lured D. citri adults by simulating the color of citrus flush and killed them with beta-cyfluthrin. This study evaluated: 1) lethality of AK devices weathered up to 8 wk on residential citrus; 2) survival of psyllids caged with potted plants and AK devices; 3) psyllid suppression achieve… Show more
“…Attract-and-kill devices or traps based on psyllid visual and gustatory behavior could be a valuable addition to practices currently available for management of D. citri 48 . Traps combining yellow color (long-range wavelength) with UV (short-range wavelength) reflectance from magnesium oxide should provide an effective combination of visual cues to promote both long-range attraction and sustainted probing behavior by psyllids.…”
Asian citrus psyllid (Diaphorina citri) vectors the bacterium Candidatus Liberibacter asiaticus, the causal pathogen of citrus greening disease that is devastating citrus industries worldwide. Suppressing psyllid populations is crucial to prevent disease spread. An attract-and-kill trap based on psyllid behavior would fill a niche for monitoring and control. To optimize visual attraction of psyllids, the ultraviolet (UV) reflective properties of magnesium oxide (MgO) and/or barium sulfate (BaSO 4) were assessed for potential application to a trap surface. Under low UV, high UV and natural sunlight conditions, the reflectance, attraction, and probing behaviors of psyllids were evaluated on surfaces containing magnesium oxide or barium sulfate. Magnesium oxide added to yellow sticky traps enhanced visual response of D. citri. Probing assays demonstrated that magnesium oxide alone or as a mixture with a phagostimulant blend, increased the UV reflectance of substrates, as well as, attraction and probing by psyllids. Results demonstrated that psyllids respond to both short (UV) and long (yellow) wavelengths during orientation, and that these inert compounds can increase UV reflectance and improve attractiveness of an attract-and-kill device.
“…Attract-and-kill devices or traps based on psyllid visual and gustatory behavior could be a valuable addition to practices currently available for management of D. citri 48 . Traps combining yellow color (long-range wavelength) with UV (short-range wavelength) reflectance from magnesium oxide should provide an effective combination of visual cues to promote both long-range attraction and sustainted probing behavior by psyllids.…”
Asian citrus psyllid (Diaphorina citri) vectors the bacterium Candidatus Liberibacter asiaticus, the causal pathogen of citrus greening disease that is devastating citrus industries worldwide. Suppressing psyllid populations is crucial to prevent disease spread. An attract-and-kill trap based on psyllid behavior would fill a niche for monitoring and control. To optimize visual attraction of psyllids, the ultraviolet (UV) reflective properties of magnesium oxide (MgO) and/or barium sulfate (BaSO 4) were assessed for potential application to a trap surface. Under low UV, high UV and natural sunlight conditions, the reflectance, attraction, and probing behaviors of psyllids were evaluated on surfaces containing magnesium oxide or barium sulfate. Magnesium oxide added to yellow sticky traps enhanced visual response of D. citri. Probing assays demonstrated that magnesium oxide alone or as a mixture with a phagostimulant blend, increased the UV reflectance of substrates, as well as, attraction and probing by psyllids. Results demonstrated that psyllids respond to both short (UV) and long (yellow) wavelengths during orientation, and that these inert compounds can increase UV reflectance and improve attractiveness of an attract-and-kill device.
“…Recently, Chow et al 2019 [6] reported suppression of D. citri with an 'attract-and-kill' (AK) device that combined visual cues with a pyrethroid toxicant (β-cyfluthrin). Building upon these results, we developed two AK device prototypes that incorporated multiple sensory cues known to affect host finding and probing behaviors of D. citri, viz.…”
Phytophagous insects, including Asian citrus psyllids (Diaphorina citri Kuwayama), use multiple sensory modalities (vision, olfaction, and gustation,) to locate and accept host plants. We explored incorporation of several sensory cues into a multi-modal attract-and-kill device (AK device) using a three-dimensional shape to increase visibility, as well as elements of color, attractant, phagostimulant, UV reflectant, and toxicant. Attraction of adult D. citri to the device was mediated by a combination of a highly reflective yellow cylinder, a UV reflectant compound (magnesium oxide), and an odorant blend as a short-range attractant. The device surface was coated with a slow-release wax matrix (SPLAT™) augmented with a phagostimulant consisting of a 3-component blend (formic acid, acetic acid, and para-cymene) and an insecticide (β-cyfluthrin). Psyllids landing on the device attempted to feed from the wax matrix, became intoxicated, died, and fell from the device. The device remained fully active over a period of 12 weeks partly because dead psyllids or nontargets did not adhere to the surface as occurs on adhesive yellow sticky cards, the industry standard. Laboratory and field assays showed that the device attracted and killed significantly more adult D. citri than ordinary yellow sticky cards. This device or a future iteration based on the design elements of this device is expected to contribute to sustainable and environmentally appropriate management of D. citri by exploiting the psyllid’s innate behavioral responses to visual, olfactory, and gustatory stimuli.
“…Diaphorina citri has only emerged as a pest insect in the USA in the last decade and more immediate tools such as synthetic insecticides were initially sought out to overcome the impending panzootic, especially in commercial citrus (Grafton-Cardwell 2006). More recently, however, attention has turned to smaller, less managed citrus groves and residential citrus (Se ´tamou et al 2020;Chow et al 2019). This has created a niche demographic of individuals who would prefer not to use synthetic insecticide control methods and keep their citrus trees and residences organic, or at the least free of pesticide residues.…”
Ten strains of entomopathogenic ascomycete fungi, sourced from commercial formulations of blastopore or conidiospore formulations, were tested in 14 different formulations in a primary acquisition/direct spray bioassay against adult Asian citrus psyllid (Diaphorina citri Kuwayama (Hemiptera: Liviidae)). The Cordyceps (Isaria) javanica Apopka 97-C (conidia) strain was used as the standard. A statistical ranking system was established in which top performing pathogenic strains were selected for further screening and eventual field trials. Modified Potter-type spray towers were utilized to deliver a range of doses of viable spores to adult D. citri in an aqueous spray consistent with the rate of spores per hectare often used in real-world spray applications. Mortality was assessed after a seven-day incubation period under controlled climate conditions reflecting those in the Lower Rio Grande Valley (LRGV) of Texas, USA. Of the 14 preparations, the strains Metarhizium anisopliae E9, C. fumosorosea Ifr9901, Beauveria bassiana ATCC 74040 and ANT-03, M. anisopliae ESALQ1037, and M. robertsii DWR2009, showed greater levels of mortality than the standard, Apopka 97-C, in the laboratory setting. Of those six, two (Ifr9901 and ANT-03) were selected for further evaluation based on efficacy, commercial availability, geographical registration, and market outlook on production.
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