In this study, the emissions from Kirloskar Direct Injection 4-stroke Diesel engine, single cylinder air-cooled, 4.4 kW, constant speed at 1500 rpm, compression ratio 17.5:1, with different blends of diesel and Refined Palmolein oil has been analyzed. Methyl Esters of refined Palmolein was transesterified with animal fat before blending with diesel. The main objective of this study is to measure the CO, HC, NOx, Smoke Emissions by varying the Injection pressure and the load. The experiments were conducted with various blends of Refined Palmolein oil and diesel (10%+90% PD, 30%+ 70% PD, and 40%+ 60% PD) at different pressures (180 bar, 210 bar, & 240 bar) and at different loads (0%, 25%, 50%, 75%, 100%). A 3-hole nozzle was used to inject the fuel. The emissions results were studied using AVL gas analyzer. The results show that there is a decrease in HC and CO and also marginal increase in NOx with exhaust temperature