Development and evaluation of two ELISA formats for the detection of β-lactoglobulin in model processed and commercial foods

Luis, Ruth de; Lavilla, María; Sánchez, Lourdes; Calvo, Miguel; Pérez, María Dolores

doi:10.1016/j.foodcont.2008.09.017

Cited by 36 publications

(9 citation statements)

References 14 publications

(19 reference statements)

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“…Thus, the milk-allergic individual may frequently misinterpret "non-dairy" as being free from milk. Salad dressings labeled as containing soybean milk were observed to have milk-positive results, because some soybean milk manufacturer commonly add 1e2% whole milk powder to soybean milk for technical purposes (De Luis et al, 2008), as well as for providing favorable taste and nutritional values.…”

Section: Discussionmentioning

confidence: 99%

“…Regardless of labeling, complete avoidance is still difficult, because food products may not be properly labeled or may contain a food allergen by mistake e e.g. an ingredient derived from a food allergen may be overlooked (De Luis, Lavilla, Sanchez, Calvo, & Perez, 2008). Also, unintentional inclusion of food allergens might arise from crosscontamination in the food production process (Holzhauser & Vieths, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Investigation of undeclared food allergens in commercial Thai food products

et al. 2012

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Investigation of undeclared food allergens in commercial Thai food products

et al. 2012

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“…Their work also demonstrated the influence of a complex food matrix on the performance of this type of assay. The development of 2 ELISA formats (indirect competitive and sandwich) was performed by de Luis and others () for the detection of β‐LG in processed foods. The competitive and sandwich systems, with LOD of 0.5 and 0.05 mg/kg, respectively, were able to detect undeclared milk ingredients in 14% of the tested commercial samples.…”

Section: Analytical Methods For the Detection Of Milk Allergens In Prmentioning

confidence: 99%

Bovine Milk Allergens: A Comprehensive Review

Villa

Costa

Oliveira

et al. 2017

Comp Rev Food Sci Food Safe

177

145

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Cow milk allergy is one of the most common food allergies in early childhood and often persists through adult life, forcing an individual to a complete elimination diet. Milk proteins are present in uncounted food products, such as cheese, yogurt, or bakery item, exposing allergic persons to a constant threat. Many efforts have been made to overcome this global problem and to improve the life quality of allergic individuals. First, proper and reliable food labeling is fundamental for consumers, but the verification of its compliance is also needed, which should rely on accurate and sensitive analytical methods to detect milk allergens in processed foods. At the same time, strategies to reduce milk allergenicity, such as immunotherapy or the use of food processing techniques to modify allergen structure, have to be extensively studied. Recent research findings on the applicability of food processing, such as heat treatment, fermentation, or high pressure, have revealed great potential in reducing milk allergenicity. In this review, significant research advances on cow milk allergy are explored, focusing on prevalence, diagnosis, and therapy. Molecular characterization of cow milk allergens and cross-reactivity with other nonbovine milk species are described, as well as the effects of processing, food matrix, and digestibility on milk allergenicity. Additionally, analytical methods for the detection of milk allergens in food are described, from immunoassays and mass spectrometry methods for protein analysis to real-time polymerase chain reaction for DNA analysis.

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“…A large number of reports regarding the development of enzyme immunoassays using peroxidase as reporter enzyme to detect toxins, pathogens, and other analyses are available. For example, in detection of Goynyautoxins, Chlamydia, Fusarium toxin, Dengue virus proteins, Hepatitis-E virus peroxidases are used [194][195][196][197][198][199]. HRP-based immunoassays for the detection of undeclared milk proteins (eg.…”

Section: Analytical Applicationsmentioning

confidence: 99%

A Comprehensive Review on Function and Application of Plant Peroxidases

Pandey¹,

Awasthi²,

Singh³

et al. 2017

Biochem Anal Biochem

261

213

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Peroxidases, one of the key antioxidant enzymes, are widely distributed in nature and catalyze oxidation of various electron donor substrates concomitant with the decomposition of H 2 O 2. The non-animal plant peroxidases (class III peroxidase) are involved in various essential physiological processes of plant growth and development throughout their life cycle. In view of the capability of peroxidases to catalyze the redox reaction for a wide range of substrates, they are considered as one of the important enzyme from the point of view of their various medicinal, biochemical, immunological, biotechnological and industrial applications. They have been successfully used for biopulping and biobleaching in the paper and textile industries. Peroxidases have also been used in organic synthesis, bioremediation, as well as various analytical applications in diagnostic kits, ELISA. Peroxidase based biosensors find application in analytical systems for determination of hydrogen peroxide, glucose, alcohols, glutamate, and choline etc. Thus, in view of array of physiological functions as well as industrial applications, the peroxidases have conquered a dominant position in research groups and become one of the most extensively studied enzymes. In this direction, the present review embodies the classification, mechanism of action, major physiological functions as well as industrial applications of plant peroxidases.

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Development and evaluation of two ELISA formats for the detection of β-lactoglobulin in model processed and commercial foods

Cited by 36 publications

References 14 publications

Investigation of undeclared food allergens in commercial Thai food products

Investigation of undeclared food allergens in commercial Thai food products

Bovine Milk Allergens: A Comprehensive Review

A Comprehensive Review on Function and Application of Plant Peroxidases

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