“…Lanes 14-24 represent a multiplex reaction with cry9Aa2 primers producing an expected 826 bp product, the 612 bp nptII product and the 1069 bp actin product. Lanes 1-8, lines transformed with pART27cab1Ac9 (#102, #107, #110, #116, #121, #122, #123 and #130 respectively); lanes 9 and 22, no DNA template control; lanes 10 and 23, non-transgenic 'Iwa' control; lane 11, 'Iwa' line I52 known to be transgenic for nptII and cry1Ac9 (positive control, ex Davidson et al, 2002); lanes 12 and 13, 100 bp molecular ruler 10380-012 and 1 kb plus molecular ruler 10787-018 (Invitrogen, Carlsbad, California) size markers respectively; lanes 14-21, lines transformed with pART27cab9Aa2 (#7, #12, #17, #18, #22, #27, #28 and #29 respectively); lane 24, 'Iwa' line DG4c known to be transgenic for nptII and cry9Aa2 (positive control, ex Meiyalaghan et al, 2004). reactions to be conveniently distinguished from a nontransgenic line. PCR products from representative lines are illustrated in Figure 2.…”