Agrobacterium-Mediated Transformation of Potato

Millam, S.

doi:10.1007/978-1-4020-2333-0_19

Cited by 6 publications

(5 citation statements)

References 45 publications

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“…The leaf disc transformation was carried out as described by [ 68 ] with some modifications. Overnight bacterial culture (OD 600 = 0.8) was pelleted at 5000× g for 20 min and was resuspended in liquid MS medium supplemented with 30 g/L sucrose, and 100 µM acetosyringone.…”

Section: Methodsmentioning

confidence: 99%

Multiplex CRISPR-Cas9 Gene-Editing Can Deliver Potato Cultivars with Reduced Browning and Acrylamide

Iqbal

Fosu-Nyarko

et al. 2023

Plants

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Storing potato tubers at cold temperatures, either for transport or continuity of supply, is associated with the conversion of sucrose to reducing sugars. When cold-stored cut tubers are processed at high temperatures, with endogenous asparagine, acrylamide is formed. Acrylamide is classified as a carcinogen. Potato processors prefer cultivars which accumulate fewer reducing sugars and thus less acrylamide on processing, and suitable processing cultivars may not be available. We used CRISPR-Cas9 to disrupt the genes encoding vacuolar invertase (VInv) and asparagine synthetase 1 (AS1) of cultivars Atlantic and Desiree to reduce the accumulation of reducing sugars and the production of asparagine after cold storage. Three of the four guide RNAs employed induced mutation frequencies of 17–98%, which resulted in deletions, insertions and substitutions at the targeted gene sites. Eight of ten edited events had mutations in at least one allele of both genes; for two, only the VInv was edited. No wild-type allele was detected in both genes of events DSpco7, DSpFN4 and DSpco12, suggesting full allelic mutations. Tubers of two Atlantic and two Desiree events had reduced fructose and glucose concentrations after cold storage. Crisps from these and four other Desiree events were lighter in colour and included those with 85% less acrylamide. These results demonstrate that multiplex CRISPR-Cas9 technology can generate improved potato cultivars for healthier processed potato products.

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Section: Methodsmentioning

confidence: 99%

Multiplex CRISPR-Cas9 Gene-Editing Can Deliver Potato Cultivars with Reduced Browning and Acrylamide

Iqbal

Fosu-Nyarko

et al. 2023

Plants

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“…Similar to callus induction medium selection, hormone selection also played a vital role in shoots induction. We also selected 4 hormones to determine the ratio of hormones (Supplement table [4][5][6] [23, [26][27][28]. Large difference in induction rate led to different concentration ratios.…”

Section: Selection Of Shoots Induction Medium For Callusmentioning

confidence: 99%

“…Infection concentration and co-cultivation time were the transformation of the factors influence based on agrobacterium infection. Some researchers also analyzed and adjusted the agrobacterium concentration, co-culture time and infection method, and finally obtained a better way [18,[27][28][29][30].…”

Section: Optimization Of Agrobacterium Infection Conditionsmentioning

confidence: 99%

Construction of Potato DM1-3-516-R44 (DM) Transgenic System Based on Agrobacterium Transformation

Zhang

Wang

Yang

et al. 2020

Preprint

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Background: The sequencing potato DM1-3-516-R44 played an irreplaceable role in the study of gene function. So far, no one research the transformation system about DM. Therefore, our experiment was studied from three aspects: plant regeneration system, optimization of agrobacterium infection conditions and the effect of hygromycin on DM. Results: A relatively suitable method for genetic transformation of DM was obtained: 1) The stem callus induction medium was MS + IAA 0.5 mg/L + 6-BA 2.0 mg/L, the leaf callus induction medium was MS + NAA 1.0mg/L + 6-BA 0.5mg/L and the shoot differentiation medium was MS + 6-BA 3.0 mg/L + ZT 0.5 mg/L. 2) The specific transformation condition was the agrobacterium concentration kept the OD600 = 0.3, and co-culture time consisted 3 days in the dark. The hygromycin concentration chose 8 mg/L to screen the transgenic plants. 3) Using hygromycin to screen about 100 transgenic shoots, 75 shoots were obtained and 53 strains were identified had target stripe by PCR technology. Conclusion: The efficiency of the transformation system we created was over 50%. It provided a good basis for the study of potato gene function.

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“…Most transformation procedures developed for potato used Agrobacterium tumefaciens (Millam 2004, 2006, Börnke et al 2007) and a variety of traits such as starch modification, insect and virus resistance, abiotic stress tolerance and production of pharmaceutical proteins have been the goals of potato biotechnology (Börnke et al 2007, Byun et al 2007. Biolistic gene transfer, a method by which foreign genes may be introduced into cells via high velocity microprojectiles (Klein et al 1988) has been used in transforming monocots such as orchids (Suwanaketchanatit et al 2007) and dicots such as soybean (El-Shemy et al 2007), but has only recently been used in potato.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Transformation of potato via Agrobacterium coated microparticle bombardment

Nguyen¹,

Dix²,

Nugent³

2010

Biol. plant.

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The transformation of potato (Solanum tuberosum L. cv. Désirée) was extended by the Agrobacterium-mediated biolistic method. Using this approach transgenic shoots could be obtained at a similar frequency to that achieved through conventional biolistics. Leaves from shoot cultures were bombarded with gold particles coated in Agrobacterium tumefaciens cells harboring a binary plasmid encoding three genes of interest in the T-DNA. Nine shoots were obtained from 20 shots, with selection of transgenic shoots on a series of media containing progressively increasing concentrations of hygromycin from 5 to 20 mg dm -3 .

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Agrobacterium-Mediated Transformation of Potato

Cited by 6 publications

References 45 publications

Multiplex CRISPR-Cas9 Gene-Editing Can Deliver Potato Cultivars with Reduced Browning and Acrylamide

Multiplex CRISPR-Cas9 Gene-Editing Can Deliver Potato Cultivars with Reduced Browning and Acrylamide

Construction of Potato DM1-3-516-R44 (DM) Transgenic System Based on Agrobacterium Transformation

Transformation of potato via Agrobacterium coated microparticle bombardment

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