Development and evaluation of lateral flow assay for sero-diagnosis of Japanese encephalitis in swine

Dhanze, Himani; Bhilegaonkar, K. N.; Kumar, C. P. Girish; Kumar, Suman; Singh, Praveen; Kumar, Ashok

doi:10.1080/10495398.2019.1602539

Cited by 14 publications

(11 citation statements)

References 14 publications

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“…67 In this line, the rapid, one-step in-situ analytical technique like Lateral flow assay was also proved for detecting the JEV antibodies. 75 In this study, NS1…”

Section: Diagnosismentioning

confidence: 77%

“…This can be occupied by advanced techniques like biosensors combined with nanoparticles like silver‐nanoparticles based optical sensing probe 68 and carbon‐nanoparticles based electrochemical biosensor strip 67 . In this line, the rapid, one‐step in‐situ analytical technique like Lateral flow assay was also proved for detecting the JEV antibodies 75 . In this study, NS1 protein was immobilised as the antigen for detection by staphylococcal protein‐A which was given 100% sensitivity.…”

Section: Detecting Techniques and Their Drawbacksmentioning

confidence: 99%

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Recent advances to overcome the burden of Japanese encephalitis: A zoonotic infection with problematic early detection

Pinapati

Tandon

Tripathi

2022

Reviews in Medical Virology

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Japanese encephalitis (JE) is a vector‐borne neurotropic disease caused by Japanese encephalitis virus (JEV) associated with high mortality rate distributed from Eastern and Southern Asia to Northern Queensland (Australia). The challenges in early detection and lack of point‐of‐care biomarkers make it the most important Flavivirus causing encephalitis. There is no specific treatment for the disease, although vaccines are licenced. In this review, we focussed on point‐of‐care biomarkers as early detection tools and developing the effective therapeutic agents that could halt JE. We have also provided molecular details of JEV, disease progression, and its pathogenesis with recent findings which might bring insights to overcome the disease burden.

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“…67 In this line, the rapid, one-step in-situ analytical technique like Lateral flow assay was also proved for detecting the JEV antibodies. 75 In this study, NS1…”

Section: Diagnosismentioning

confidence: 77%

Section: Detecting Techniques and Their Drawbacksmentioning

confidence: 99%

Recent advances to overcome the burden of Japanese encephalitis: A zoonotic infection with problematic early detection

Pinapati

Tandon

Tripathi

2022

Reviews in Medical Virology

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“…The fabricated LFA consisted of AuNPs-Ag on the conjugate pad with staphylococcal protein A coated on test line and anti-antigen IgG as control line. The LFA was able to successfully detect JEV Ab in 500 clinical pig serum real samples with 100% relative diagnostic specificity as well as sensitivity in monsoon and post-monsoon (Dhanze et al 2019 ). Another recently developed LFA using AuNPs conjugated with JEV NS1 Ab as immunochromatic probes was fabricated for successful point of care detection of JEV NS1 Ag in clinical pig serum samples with a visual LOD of 10 pg/ml (Roberts et al 2022d ).…”

Section: Biosensors For Jev Diagnosticsmentioning

confidence: 99%

A brief review on novel biomarkers identified and advanced biosensing technologies developed for rapid diagnosis of Japanese Encephalitis Virus

Roberts

Gandhi

2022

Proc.Indian Natl. Sci. Acad.

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Advanced biosensor technology research is imperative for the management of infectious disease outbreaks such as Japanese Encephalitis (JE), a zoonotic disease caused by the flavivirus JE virus (JEV) which is transmitted to humans (dead-end hosts) from the amplification host, pigs, via mosquitoes. To avoid future pandemic scenarios, proactive research rather than responsive research in the field of diagnostics is a requirement for development of rapid, sensitive and specific screening detection methods. In this mini-review, we have critically compared and evaluated the different types of biomarkers (antigen, antibody, nucleic acid) identified for JEV diagnostics and their specific roles in the manifestation of the infection which may be potentially used for therapeutics and drug development as no treatment is available for JE. Furthermore, different biosensors developed for the detection of JEV biomarkers have been discussed in detail to give an overview of the working principles (electrochemical, optical, etc.), fabrication components (signal amplifier, bioreceptor, etc.), detection limits and response times. This review provides a compact compiled base on available JEV diagnostic research work being currently carried out along with their limitations, future prospective, and major challenges faced. This will enable future development of rapid point-of-care diagnostic screening methods for JEV infection management, which may help reduce number of fatalities.

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“…However, due to the required laboratory setup for signal current detection, there is an absence of on‐field electrochemical sensing devices for JEV 16 . Currently, the only commercial point‐of‐care (PoC) diagnostic assays available for JEV are enzyme‐based immunoassays and lateral flow assay (LFA) based kits which target IgM/IgG antibodies (Ab) against JEV 17,18 . The drawback of antibody targeted detection is that Abs develop in the body after Day 4/5 of infection unlike the whole virus and/or viral antigen (Ag) present from Day 1 19 resulting in delayed diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Point‐of‐care detection of Japanese encephalitis virus biomarker in clinical samples using a portable smartphone‐enabled electrochemical “Sensit” device

Roberts

Dhanze

Sharma

et al. 2023

Bioengineering & Transla Med

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Japanese encephalitis (JE), a neglected tropical zoonotic disease prevalent in south‐east Asian and western pacific countries, caused by the flavivirus JE virus (JEV), has a dearth of electrochemical point‐of‐care (PoC) diagnostic tools available to manage endemic breakouts. To overcome this, we have developed a screen‐printed carbon electrode (SPCE) immunosensor for rapid PoC detection of JEV nonstructural 1 (NS1) antigen (Ag), found circulating in serum of infected individuals using a smartphone based portable “Sensit” device. The modification of SPCE surface with JEV NS1 antibody (Ab) was confirmed via observation of globular protein structures via scanning electron microscopy (SEM), increase in electrode surface hydrophilicity via contact angle measurement and decrease in current via differential pulse voltammetry (DPV). The fabrication and testing parameters were optimized based on highest current output obtained using DPV. The SPCE was tested for detection limit of target JEV NS1 Ag ranging from 1 fM to 1 μM, which was determined as 0.45 fM in spiked serum. The disposable immunosensor was also found to be highly specific in detecting JEV NS1 Ag over other flaviviral NS1 Ag. Finally, the modified SPCE was clinically validated by testing 62 clinical JEV samples using both a portable miniaturized electrochemical “Sensit” device coupled with a smartphone and a laboratory‐based potentiostat. The results were corroborated with gold‐standard RT‐PCR and showed 96.77% accuracy, 96.15% sensitivity, and 97.22% specificity. Hence, this technique may further be developed into a one‐step rapid diagnostic tool for JEV, especially in rural areas.

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Development and evaluation of lateral flow assay for sero-diagnosis of Japanese encephalitis in swine

Cited by 14 publications

References 14 publications

Recent advances to overcome the burden of Japanese encephalitis: A zoonotic infection with problematic early detection

Recent advances to overcome the burden of Japanese encephalitis: A zoonotic infection with problematic early detection

A brief review on novel biomarkers identified and advanced biosensing technologies developed for rapid diagnosis of Japanese Encephalitis Virus

Point‐of‐care detection of Japanese encephalitis virus biomarker in clinical samples using a portable smartphone‐enabled electrochemical “Sensit” device

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