Development and evaluation of analytical methodology for the determination of aflatoxins in palm kernels

Nawaz, S.; Coker, R. D.; Haswell, Stephen J.

doi:10.1039/an9921700067

Cited by 15 publications

(7 citation statements)

References 8 publications

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“…The HPTLC has since overcome the problems associated with the conventional TLC techniques through automation of sample application, development, and plate interpretation. It is not surprising that currently HPTLC is one of the most efficient and precise methods in aflatoxins analysis [64,65]. Nevertheless, the requirement for skilled operators, the costs of the equipment coupled with its bulkiness, and the extensive sample pretreatment limit the HPTLC to the laboratory and thus it is inapplicable in field situations.…”

Section: Thin-layer Chromatography (Tlc)mentioning

confidence: 99%

Methods for Detection of Aflatoxins in Agricultural Food Crops

Wacoo

Wendiro²,

Vuzi

et al. 2014

Journal of Applied Chemistry

200

149

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Aflatoxins are toxic carcinogenic secondary metabolites produced predominantly by two fungal species: Aspergillus flavus and Aspergillus parasiticus. These fungal species are contaminants of foodstuff as well as feeds and are responsible for aflatoxin contamination of these agro products. The toxicity and potency of aflatoxins make them the primary health hazard as well as responsible for losses associated with contaminations of processed foods and feeds. Determination of aflatoxins concentration in food stuff and feeds is thus very important. However, due to their low concentration in foods and feedstuff, analytical methods for detection and quantification of aflatoxins have to be specific, sensitive, and simple to carry out. Several methods including thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), mass spectroscopy, enzyme-linked immune-sorbent assay (ELISA), and electrochemical immunosensor, among others, have been described for detecting and quantifying aflatoxins in foods. Each of these methods has advantages and limitations in aflatoxins analysis. This review critically examines each of the methods used for detection of aflatoxins in foodstuff, highlighting the advantages and limitations of each method. Finally, a way forward for overcoming such obstacles is suggested.

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Section: Thin-layer Chromatography (Tlc)mentioning

confidence: 99%

Methods for Detection of Aflatoxins in Agricultural Food Crops

Wacoo

Wendiro²,

Vuzi

et al. 2014

Journal of Applied Chemistry

200

149

View full text Add to dashboard Cite

show abstract

“…Nawaz and others (1992) have developed a rapid, simple, and reproducible method for the simultaneous determination of aflatoxins (AFB1, AFB2, AFG1, and AFG2) in palm kernel samples by optimizing sample preparation, solvent extraction, clean‐up, and quantification procedures. Aflatoxins in the samples are extracted from palm kernel slurries with a mixture of acetone–water (80 + 20, v/v) and the crude extract is cleaned by solid‐phase extraction using a phenyl‐bonded phase cartridge.…”

mentioning

confidence: 99%

Mycotoxins in Food and Feed: Present Status and Future Concerns

Bhat

Ravishankar

Karim

2009

Comp Rev Food Sci Food Safe

547

382

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Disease outbreaks due to the consumption of contaminated food and feedstuff are a recurring problem worldwide. The major factor contributing to contamination are microorganisms, especially fungi, which produce low-molecular-weight compounds as secondary metabolites, with confirmed toxic properties referred to as mycotoxins. Several mycotoxins reported to date are cosmopolitan in distribution and incur severe health-associated risks (including cancer and neurological disorders). Hence, creating awareness among consumers, as well as developing new methods for detection and inactivation is of great importance for food safety. In this review, the focus is on the occurrence of various types of mycotoxins in food and feed associated with risks to humans and livestock, as well as legislation put forth by various authorities, and on presently practiced detoxification methods. Brief descriptions on recent developments in mycotoxin detection methodology are also inlcuded. This review is meant to be informative not only for health-conscious consumers but also for experts in the field to pave the way for future research to fill the existing gaps in our knowledge with regard to mycotoxins and food safety.

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“…The conventional TLC method has improved through the automation of sample spotting, plate development and interpretation in HPTLC. Currently, HPTLC is one of the best analytical methods for estimation of aflatoxins [60,61].…”

Section: High Performance Thin Layer Chromatography (Hptlc)mentioning

confidence: 99%

Chromatographic Techniques for Estimation of Aflatoxins in Food Commodities

Abbas¹

2022

Aflatoxins - Occurrence, Detoxification, Determination and Health Risks

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Aflatoxins, produced mainly by Aspergillus flavus Aspergillus parasiticus, have been documented as one of the major food contaminants throughout the world. Because of their toxic nature, these food contaminants have acknowledged considerable attention in recent years. Among the different types of Aflatoxins, the most prevalent and predominant Aflatoxins are AFB1, AFB2, AFG1, AFG2, AFM1, AFM2 which are considered the more lethal as compared to others. Several analytical and immunological methods are available for testing and estimating aflatoxins in different food commodities. However, chromatographic techniques have been considered superior regarding the estimation of aflatoxins both qualitatively and quantitatively. Chromatographic techniques have numerous applications for the separation and identification of chemical and biological compounds in food industry. It has grown to be the most popular and versatile of all analytical techniques in laboratories used for the analysis of multiple components in different matrices. For preliminary qualitative detection of Aflatoxins, Thin layer chromatography (TLC) is considered the best analytical technique which is being used broadly in food industry. However, liquid chromatographic techniques including High Performance Liquid Chromatography (HPLC) and Liquid chromatography-mass Spectrometry (LC–MS) are the best analytical techniques developed so far for the quantification of Aflatoxins in food commodities.

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Development and evaluation of analytical methodology for the determination of aflatoxins in palm kernels

Cited by 15 publications

References 8 publications

Methods for Detection of Aflatoxins in Agricultural Food Crops

Methods for Detection of Aflatoxins in Agricultural Food Crops

Mycotoxins in Food and Feed: Present Status and Future Concerns

Chromatographic Techniques for Estimation of Aflatoxins in Food Commodities

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