Development and evaluation of a multicomponent bioink consisting of alginate, gelatin, diethylaminoethyl cellulose and collagen peptide for 3D bioprinting of tissue construct for drug screening application

Geevarghese, Rency; Somasekharan, Lakshmi T.; Bhatt, Anugya; Kasoju, Naresh; Nair, Renjith P.

doi:10.1016/j.ijbiomac.2022.02.191

Cited by 18 publications

(9 citation statements)

References 51 publications

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“…While we and others have presented bioprinted models composed of lung cancer cells by extrusion-based technologies before [ 21 , 40 , 41 ], the present study is, to the best of our knowledge, the first lung cancer model produced by DLP bioprinting. The particularly high resolution of this methodology allowed the inclusion of the predesigned vascular structure.…”

Section: Discussionmentioning

confidence: 94%

“…Due to its high flexibility, the technology allows the adjustment of the stiffness of the extracellular environment, the combination of various cell types, and the design of desired 3D arrangements. In a recent lung cancer model, a multicomponent bioink composed of alginate, diethylaminoethyl cellulose, gelatin, and collagen peptide was used to generate a 3D bioprinted construct, and the model was shown to be suitable for initial drug screening [ 21 ]. Virtually all of the bioprinted cancer models are composed of human cancer cells.…”

Section: Introductionmentioning

confidence: 99%

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Generation of a Perfusable 3D Lung Cancer Model by Digital Light Processing

Mei

Berg

et al. 2023

IJMS

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Lung cancer still has one of the highest morbidity and mortality rates among all types of cancer. Its incidence continues to increase, especially in developing countries. Although the medical field has witnessed the development of targeted therapies, new treatment options need to be developed urgently. For the discovery of new drugs, human cancer models are required to study drug efficiency in a relevant setting. Here, we report the generation of a non-small cell lung cancer model with a perfusion system. The bioprinted model was produced by digital light processing (DLP). This technique has the advantage of including simulated human blood vessels, and its simple assembly and maintenance allow for easy testing of drug candidates. In a proof-of-concept study, we applied gemcitabine and determined the IC50 values in the 3D models and 2D monolayer cultures and compared the response of the model under static and dynamic cultivation by perfusion. As the drug must penetrate the hydrogel to reach the cells, the IC50 value was three orders of magnitude higher for bioprinted constructs than for 2D cell cultures. Compared to static cultivation, the viability of cells in the bioprinted 3D model was significantly increased by approximately 60% in the perfusion system. Dynamic cultivation also enhanced the cytotoxicity of the tested drug, and the drug-mediated apoptosis was increased with a fourfold higher fraction of cells with a signal for the apoptosis marker caspase-3 and a sixfold higher fraction of cells positive for PARP-1. Altogether, this easily reproducible cancer model can be used for initial testing of the cytotoxicity of new anticancer substances. For subsequent in-depth characterization of candidate drugs, further improvements will be necessary, such as the generation of a multi-cell type lung cancer model and the lining of vascular structures with endothelial cells.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Generation of a Perfusable 3D Lung Cancer Model by Digital Light Processing

Mei

Berg

et al. 2023

IJMS

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“…A polysaccharide bioink filled with neural stem cells (NSCs) and made of hyaluronic acid derivatives, functional chitosan, and matrigel was successfully 3D-bioprinted to create neural tissue structures for spinal cord injury repair. 49 Geevarghese et al 40 used a multicomponent bioink consisting of gelatin, alginate, diethylaminoethyl cellulose, and collagen peptides to 3D-bioprint a lung cancer tumor model to observe the efficacy of the constructs in drug screening applications. Collagen, fibrin, and gelatin are the main ECM proteins of the ECM.…”

Section: Composition Of Bioinksmentioning

confidence: 99%

3D bioprinting tumor models mimic the tumor microenvironment for drug screening

Yue

et al. 2023

Biomater. Sci.

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“…However, there are different emphases in constructing primary and metastatic tumor models. For primary tumor models, they tend to explore the growth status of tumors in 3D TME environments or construct high-throughput models for large-scale drug screening. , Combining patient-derived tumors with 3D bioprinting technology to construct particular tumor models for personalized medical treatment is also one of the research directions for in situ tumor models . For metastatic tumor models, it is critical to reproduce the metastatic behavior of tumor cells in the context of restoring the tumor microenvironment.…”

Section: D Bioprinting Technologymentioning

confidence: 99%

3D Bioprinting for Tumor Metastasis Research

Lin

Tang

Duan

et al. 2023

ACS Biomater. Sci. Eng.

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Tumor metastasis is a multiple cascade process where tumor cells disseminate from the primary site to distant organs and subsequently adapt to the foreign microenvironment. Simulating the physiology of tumor metastatic events in a realistic and three-dimensional (3D) manner is a challenge for in vitro modeling. 3D bioprinting strategies, which can generate well-customized and bionic structures, enable the exploration of dynamic tumor metastasis process in a species-homologous, high-throughput and reproducible way. In this review, we summarize the recent application of 3D bioprinting in constructing in vitro tumor metastatic models and discuss its advantages and current limitations. Further perspectives on how to harness the potential of accessible 3D bioprinting strategies to better model tumor metastasis and guide anti-cancer therapies are also provided.

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Development and evaluation of a multicomponent bioink consisting of alginate, gelatin, diethylaminoethyl cellulose and collagen peptide for 3D bioprinting of tissue construct for drug screening application

Cited by 18 publications

References 51 publications

Generation of a Perfusable 3D Lung Cancer Model by Digital Light Processing

Generation of a Perfusable 3D Lung Cancer Model by Digital Light Processing

3D bioprinting tumor models mimic the tumor microenvironment for drug screening

3D Bioprinting for Tumor Metastasis Research

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