Development and characterization of SSR markers in <i>Ribes</i> species

Brennan, Rex; Jorgensen, Linzi; Woodhead, M.; Russell, Joanne

doi:10.1046/j.1471-8286.2002.00233.x

Cited by 34 publications

(33 citation statements)

References 11 publications

(11 reference statements)

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“…Therefore, this study could be of considerable help in promoting genetic resource conservation and utilization as well as plant variety protection. The development and potential use of molecular markers in currant and related Ribes species has been previously reported, using inter-simple sequence repeats (Lanham et al, 2000), AFLPs (Lanham and Brennan, 1999) and microsatellites (SSRs) (Brennan et al, 2002), and SNP (Russell et al, 2011). However, no efficient strategy to apply DNA markers easily for currant variety identification had been reported.…”

Section: Discussionmentioning

confidence: 99%

A new strategy for identification of currant (Ribes nigrum L.) cultivars using RAPD markers

Huo

Yang²,

Zhang³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. We developed a new approach using RAPD fingerprints to distinguish 37 currant cultivars from northeastern China based on optimization of RAPD by choosing 11 nucleotide primers and strict screening PCR annealing temperature. We found that the manual cultivar identification diagram (MCID) approach clearly developed fingerprints from 8 different primers that were useful for cultivar identification; a cultivar identification diagram (CID) was readily constructed. This CID allows efficient currant cultivar identification, providing information to separate all the currant cultivars from each other, based on the detail polymorphic bands from the corresponding primers, which were marked in the correct positions on the currant CID. According to the CID, 10 currant cultivars in 5 groups were randomly selected for the referable and workable identification of this strategy. The results proved the workability and efficiency of the MCID method, facilitating the identification of fruit cultivars with DNA markers. This MCID approach will be useful for early identification of seedlings in the nursery industry and protection of cultivar rights.

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Section: Discussionmentioning

confidence: 99%

A new strategy for identification of currant (Ribes nigrum L.) cultivars using RAPD markers

Huo

Yang²,

Zhang³

et al. 2013

Genet. Mol. Res.

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“…SSRs were identified using the method described in Brennan et al (2002), which is a modification of the method described by Edwards et al (1996) and Hale et al (2001). Primers were designed to SSRs and tested for polymorphism using parental DNA and mapped in the segregating population following the protocol described in Brennan et al (2002).…”

Section: Genomic Ssr Developmentmentioning

confidence: 99%

“…Primers were designed to SSRs and tested for polymorphism using parental DNA and mapped in the segregating population following the protocol described in Brennan et al (2002).…”

Section: Genomic Ssr Developmentmentioning

confidence: 99%

“…Phagemids (pBluescript) containing cDNA inserts were obtained by mass excision as described by the manufacturer (Stratagene). Transformed colonies were picked and grown up for phagemid DNA preparation using the protocol described for plasmid DNA in Brennan et al (2002). Four · 384 well plates of phagemid were single pass sequenced using the M13 reverse primer and BigDye Terminator v 3.1 cycle sequencing kit (Applied Biosystems) and were analysed on an ABI PRISM 3700 automated sequencer (Applied Biosystems, Foster City, CA, USA).…”

Section: Generation Of Est--ssr Markersmentioning

confidence: 99%

“…The development and potential use of molecular markers in Ribes has been previously reported, using inter-simple sequence repeats (Lanham et al 2000), AFLPs (Lanham and Brennan 1999) and microsatellites (SSRs) (Brennan et al 2002). The latter offer breeders considerably more scope in the genetic analysis of individuals within the programme, and the study reported here uses both genomic and ESTderived SSRs (Scott 2001;Woodhead et al 2003), the latter derived from expressed sequence tags from a Ribes cDNA library (Woodhead et al 1998).…”

Section: Introductionmentioning

confidence: 99%

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The development of a genetic linkage map of blackcurrant (Ribes nigrum L.) and the identification of regions associated with key fruit quality and agronomic traits

Brennan¹,

Jorgensen²,

Hackett

et al. 2007

Euphytica

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The first genetic linkage map of blackcurrant (Ribes nigrum L.) was constructed using AFLP, SSR (genomic and EST-derived) and SNP markers, in a mapping population derived from two diverse breeding clones of blackcurrant from the SCRI breeding programme. Cluster analysis of the population revealed that the individuals within the population formed two distinct sub-populations, with segregation ratios consistent with one sub-population having the two intended parents, and the other being selfed segregants. The latter sub-population improves the map by providing a more informative estimate of recombination frequency than the crossed sub-population for some marker configurations, and also revealed the presence of two unlinked loci affecting viability. Several important phenological, agronomic and fruit quality traits were evaluated in the mapping population, and QTLs affecting these are located on the linkage map. This provides a framework for the development of marker-assisted breeding strategies for blackcurrant, to improve breeding efficiency and time to cultivar.

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Currants and Gooseberries

Brennan¹

Temperate Fruit Crop Breeding

103

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The cultivation of Ribes fruits (black-and redcurrants and gooseberries) is aimed at both fresh and processing markets, with the blackcurrant R. nigrum particularly important in the latter. Breeding is increasingly focused on national and regional requirements, and for blackcurrant the specific quality requirements of the processing industry are key objectives in many programmes, alongside agronomic traits such as yield and pest resistance. Durable resistance to foliar pathogens and damaging pests such as gall mite remains a high priority, partly due to increasing interest in integrated crop management systems. Many Ribes species, especially the darker-fruited blackcurrant types, contain high concentrations of polyphenolic compounds, notably anthocyanins and flavonols, and these components are of growing importance due to their link to human health, together with high levels of ascorbic acid (vitamin C). The development of marker-assisted breeding strategies in Ribes is in progress, to improve breeding efficiency and time to cultivar, and QTLs affecting several important phenological, agronomic and fruit quality traits have been located on the recently-developed linkage map for blackcurrant. Additionally, markers linked to key traits such as gall mite resistance are under development.

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Development and characterization of SSR markers in Ribes species

Cited by 34 publications

References 11 publications

A new strategy for identification of currant (Ribes nigrum L.) cultivars using RAPD markers

A new strategy for identification of currant (Ribes nigrum L.) cultivars using RAPD markers

The development of a genetic linkage map of blackcurrant (Ribes nigrum L.) and the identification of regions associated with key fruit quality and agronomic traits

Currants and Gooseberries

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