Development and Characterization of New Microsatellite Markers for the Oyster Mushroom (Pleurotus ostreatus)

Abstract

We developed and characterized 36 polymorphic microsatellite markers for the oyster mushroom (Pleurotus ostreatus). In total, 169 alleles were identified with an average of 4.7 alleles per locus. Values for observed (H O) and expected (H E) heterozygosities ranged from 0.027 to 0.946 and from 0.027 to 0.810, respectively. Nineteen loci deviated from Hardy-Weinberg equilibrium. Significant (P<0.05) excess heterozygosity was observed at nine loci. Linkage disequilibrium (LD) was significant (P<0.05) between pairs of locus alleles. Cluster analysis revealed that five species of genus Pleurotus made a distinct group, and the individual cultivars were grouped into major five groups from G-1 to G-5. The diverse cultivars of P. ostreatus were discriminated and the other four species revealed a different section in the UPGMA tree. These microsatellite markers proved to be very useful tools for genetic studies, including assessment of the diversity and population structure of P. ostreatus.

Development of 44 Novel Polymorphic SSR Markers for Determination of Shiitake Mushroom (Lentinula edodes) Cultivars

“…citrinopileatus , and 1 P . nebrodensis ) [28]. The SSR markers developed in this study showed similar diversity values with the SSR markers of other edible mushrooms.…”

Novel microsatellite markers suitable for genetic studies in Polyporus umbellatus (Polyporales, Basidiomycota)

“…Previous studies of the genetic diversity of other macromycetes using genomic-SSRs reported an average of 4.7e5.6 alleles per locus with average genetic diversity values of 0.35e0.4. In this study, we observed a similar average of 9.875 alleles per locus and a higher average gene diversity value of 0.544 using genic SSR markers (Foulongne-Oriol et al, 2009;Ma et al, 2009). This may be due to the deep sequencing data we obtained.…”

Using SSR markers to evaluate the genetic diversity of Lentinula edodes’ natural germplasm in China

“…Several reports on SSR used in Suillus bovines, Pleurotus ostreatus, and Agaricus bisporus, confirmed that microsatellite markers were essentially useful for genetic studies on mushroom (Kikuchi et al 2007;Foulongne-Oriol et al 2009;Kyung-Ho et al 2009). So, it is necessary to set up such an excellent marker technique in L. edodes, an economically important mushroom in the world.…”