Using SSR markers to evaluate the genetic diversity of Lentinula edodes’ natural germplasm in China

Abstract

In this study, microsatellite markers were utilized to reveal the genetic diversity of 56 strains of Lentinula edodes grown in China. A total of 224 DNA bands were detected through 25 primer pairs, of which, 223 bands (99.6%) were polymorphic between two or more strains. The variation in SSR (Simple Sequence Repeat) DNA band patterns and average genetic similarity among the wild strains of L. edodes obtained from the same region uncovered a vast genetic diversity in the natural germplasm found in China. Compared with L. edodes strains from other areas, the genetic diversity of those from the Yunnan Plateau, Hengduanshan mountains, Taiwan, and south China was significantly greater. Based on cluster analysis and principal coordinate analysis, the results indicated that all L. edodes strains could be divided into three major groups. These results effectively displayed the differences between the strains from North and South China, and those from the same or adjoining regions could cluster preferentially into small groups in most cases, suggesting the positive correlation between the cluster results and the geographical origin for the natural germplasm of Chinese L. edodes. To our knowledge, this is a pioneering report on the utilization of the SSR marker technique in analyzing L. edodes' genetic diversity.

Applying target region amplification polymorphism markers for analyzing genetic diversity of Lentinula edodes in China

“…In comparison, the genetic diversity of shiitake strains from Northwest, East and Central China was relatively low. The results of the present study were overall consistent with those of previous RAPD and SSR studies [17,26], which confirmed that the natural germplasms of L. edodes grown on the Yunnan Plateau, in the Hengduanshan Mountains, and in Taiwan and South China possess rich genetic diversity.…”

“…All the strains of L. edodes used in the present TRAP analysis were identical to those used in a previous SSR analysis [26]. The results between the two analyses were overall complementary, which verified the reliability of the study on the genetic diversity of wild shiitake strains in China.…”

“…A previous AFLP analysis generated 20 -160 DNA fragments per primer pair [29], which was similar to that of our TRAP analysis. In contrast to the previous genetic diversity studies on L. edodes natural germplasm using RAPD, SSR and internal transcribed spacer (ITS) sequence analyses [17,26,30], which were only able to investigate 147 loci, 224 loci, and ITS genes of approximately 700 bp, respectively, TRAP analysis attained 932 loci, which could provide more genetic information for assessing the genetic relationship among the tested strains of L. edodes. The present results demonstrated that TRAP is a simple yet powerful technique for estimating the genetic diversity of L. edodes.…”

Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes

“…Total genomic DNA was extracted from the freeze-dried mycelia using the cetyltrimethylammonium bromide (CTAB) procedure (Murray & Thompson 1980). DNA was then detected as previously described (Xiao et al 2010) and was diluted to 50 ng/mL.…”

Development of IRAP-SCAR marker for strain identification in Lentinula edodes

“…Additional 55 wild strains in mainland and one Taiwan commercial strains of L. edodes were used to test the validity of developed SCAR marker (Xiao et al 2010). All the tested strains were stored in Spawn Test Center of Huazhong Agricultural University.…”