Development and characterization of microsatellite markers for the wild South American <i>Passiflora cincinnata</i> (Passifloraceae)

Cerqueira-Silva, Carlos Bernard Moreno; Santos, Elisa Susilene Lisboa dos; Souza, Alisson M.; Mori, Gustavo Maruyama; Oliveira, E. J. de; Corrêa, Ronan Xavier; Souza, Anete Pereira de

doi:10.3732/ajb.1100477

Cited by 26 publications

(40 citation statements)

References 8 publications

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“…The first isolation and characterization of microsatellite markers in Passiflora were reported by Oliveira et al (2005) and Padua et al (2005) from yellow passion fruit (P. edulis f. flavicarpa) and fragrant granadilla (P. alata Curtis) from Brazilian accessions. New microsatellite markers for wild and commercial species of Passiflora reported by Cerqueira et al (2012Cerqueira et al ( , 2014a confirmed that these are useful tools to understand the mating system and hybridization within the genus. A study performed by Santos et al (2011) using ISSR markers in P. edulis (purple and yellow type) and P. alata accessions showed that there is no structure in the populations evaluated, although the results provide practical information for parental selection to assist breeding.…”

Section: Introductionmentioning

confidence: 80%

Variability and genetic structure of yellow passion fruit (Passiflora edulis f. flavicarpa Degener) in Colombia using microsatellite DNA markers

Ocampo¹,

Acosta-Barón²,

Fernández³

2017

Agron. colomb.

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Colombia is one of the leading producers of yellow passion fruit but the genetic studies based on molecular markers from commercial plantations have not been considered to select interesting market material. The goal of this study was to assess the genetic variability and the population structure of 51 Colombian commercial yellow passion fruit accessions (102 individuals), and to provide the necessary information for prospective selection and breeding programs. Thus, a total of six microsatellites were amplified with 58 alleles identified and an average of 9.66 alleles per locus, including nine private and 31 rare. Diversity indexes showed polymorphic information content values of 0.74 (PIC), an observed (Ho) and expected (He) heterozygosity average of 0.52 and 0.78, respectively. Spatial distribution showed the greatest allelic richness (11 to 14) in most of the Valle del Cauca accessions. The average genetic distance among accessions was 0.68, and the cluster analysis showed three main groups poorly supported (bootstrap <50%), with slight geographical structure and high differentiation between individuals of the same accession. Structure analysis indicated K=4 as the genetic structure's uppermost hierarchical level, while Bayesian clustering showed a division of individuals into four genetically distinct groups. The low geographic structure and high variability of the accessions could be explained by allogamy and seed exchange frequency among farmers. Results issued suggest a complementary agro-morphological assessment to establish total genetic variability and implement a breeding program through assisted selection of superior genotypes in search of more productive and resistant cultivars to phytosanitary problems.

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Section: Introductionmentioning

confidence: 80%

Variability and genetic structure of yellow passion fruit (Passiflora edulis f. flavicarpa Degener) in Colombia using microsatellite DNA markers

Ocampo¹,

Acosta-Barón²,

Fernández³

2017

Agron. colomb.

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“…1) (Supplementary Material-SM 1). The accessions encompassed popularly known variations, such as purple and yellow passion fruits, both of which are found in P. edulis Sims (Bernacci et al 2008 A set of 23 SSR loci was used, 11of which came from theseries mPc-UNICAMP, mPe-UNICAMP and mPs-UNICAMP (Cerqueira- Silva et al 2012bSilva et al , 2014b and 12 fromthe series USP (Oliveira 2006). Polymerase chain reaction (PCR) assays to obtain diversity estimates were conducted in a final volume of 15 lL, containing 12 ng of template DNA, 0.4 mM each primer (reverse and forward) and 1.0 U of Taq DNA polymerase (Invitrogen Co, Carlsbad, CA, USA).…”

Section: Biological Materialsmentioning

confidence: 99%

“…Molecular markers have also contributed to the monitoring of genetic variability in segregant populations subjected to cycle selection (Reis et al 2012(Reis et al , 2011Costa et al 2012) and backcrossing (Fonseca et al 2009). These referenced works, together with the growing number of results concerning the identification and characterization of microsatellite markers in passion fruit species (Cerqueira- Silva et al 2014b;Penha et al 2013;Cazé et al 2012;Cerqueira-Silva et al 2012b;Padua et al 2005;Oliveira et al 2005) should effectively contribute to advancing breeding programs and to proposing strategies for managing and conserving passion fruit.…”

Section: Introductionmentioning

confidence: 99%

Characterization and selection of passion fruit (yellow and purple) accessions based on molecular markers and disease reactions for use in breeding programs

et al. 2014

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Passiflora edulis Sims, which is native to South America, stands out as a passion fruit species with major potential for fruit production and marketing. This species is popularly known as yellow or purple passion fruit, depending on the color of the fruits produced. Brazil is the major worldwide producer of passion fruit; however, the average productivity of the country is low compared with its potential for culture. Fungal, bacterial and viral pathogens are among the factors limiting the productivity of passion fruit. Furthermore, no existing cultivars exhibit both productivity and resistance to disease. To select genetic material that will be useful for core collections and for increasing the genetic resistance of passion fruit cultivars to pathogens, we characterized 36 accessions based on 23 microsatellite loci and six variables related to the reactions to three diseases (woodiness virus, scab and anthracnose). We identified 127 alleles (an average of 5.52 alleles per locus), 30 % of which were private for yellow or purple passion fruit accessions. Analysis of variance and mean comparison tests indicated differences in five of the six variables (p \ 0.05, Scott-Knott test). Differences between the average reactions of the yellow and purple passion fruit accessions were also observed for the symptoms of woodiness virus and anthracnose (p \ 0.05, Mann-Whitney test).Together with these results, molecular and phenotypic estimates allowed the identification of groups of preferential accessions for use in breeding programs, for example, accessions BGP029, 071, 168, 205 and 277.

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“…Microsatellite markers have been developed for some species of the genus Passiflora, and for P. edulis Sims , P. alata Curtis , P. cincinnata Mast. (Cerqueira-Silva et al, 2012), and P. contracta Vitta (Cazé et al, 2012). However, microsatellites have not been developed yet for most species of this genus.…”

Section: Introductionmentioning

confidence: 99%

Genetic variability assessment in the genus Passiflora by SSR marker

Paiva¹,

Viana²,

Santos³

et al. 2014

Chilean J. Agric. Res.

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Development and characterization of microsatellite markers for the wild South American Passiflora cincinnata (Passifloraceae)

Abstract: The microsatellite markers identified in this study may be valuable tools for population genetic studies, and this set of markers also may be useful in the design of a genetic conservation strategy, mating system, and hybridization studies.

Cited by 26 publications

References 8 publications

Variability and genetic structure of yellow passion fruit (Passiflora edulis f. flavicarpa Degener) in Colombia using microsatellite DNA markers

Variability and genetic structure of yellow passion fruit (Passiflora edulis f. flavicarpa Degener) in Colombia using microsatellite DNA markers

Characterization and selection of passion fruit (yellow and purple) accessions based on molecular markers and disease reactions for use in breeding programs

Genetic variability assessment in the genus Passiflora by SSR marker

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