Development and characterization of microsatellite markers in a clonal plant, <i>Dioscorea japonica</i> Thunb.

Mizuki, Inoue; Tani, Naoki; Ishida, Kiyoshi; Tsumura, Yoshihiko

doi:10.1111/j.1471-8286.2005.01020.x

Cited by 16 publications

(11 citation statements)

References 11 publications

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“…Seventy-five SSRs developed from five different yam species – D. rotundata , D. abyssinica , D. prahensilis , D. japonica and D. alata (Misuki et al , 2005; Tostain et al , 2006; Andris et al , 2010) – were used to determine the genotypes of the genitors. Only the six SSR markers that revealed polymorphism between the diploid parents ‘5F’ and ‘Kabusa’, without any common allele (Da2F10, Da1D08, mDaCIR8, mDaCIR60, mDaCIR61 and mDrCIR128), were selected.…”

Section: Methodsmentioning

confidence: 99%

Microsatellite and flow cytometry analysis to help understand the origin of Dioscorea alata polyploids

Némorin

David²,

Maledon

et al. 2013

Annals of Botany

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Background and Aims Dioscorea alatais a polyploid species with a ploidy level ranging from diploid (2n = 2x = 40) to tetraploid (2n = 4x = 80). Ploidy increase is correlated with better agronomic performance. The lack of knowledge about the origin of D. alata spontaneous polyploids (triploids and tetraploids) limits the efficiency of polyploid breeding. The objective of the present study was to use flow cytometry and microsatellite markers to understand the origin of D. alata polyploids.MethodsDifferent progeny generated by intracytotype crosses (2x × 2x) and intercytotype crosses (2x × 4x and 3x × 2x) were analysed in order to understand endosperm incompatibility phenomena and gamete origins via the heterozygosity rate transmitted to progeny.ResultsThis work shows that in a 2x × 2x cross, triploids with viable seeds are obtained only via a phenomenon of diploid female non-gametic reduction. The study of the transmission of heterozygosity made it possible to exclude polyspermy and polyembryony as the mechanisms at the origin of triploids. The fact that no seedlings were obtained by a 3x × 2x cross made it possible to confirm the sterility of triploid females. Flow cytometry analyses carried out on the endosperm of seeds resulting from 2x × 4x crosses revealed endosperm incompatibility phenomena.ConclusionsThe major conclusion is that the polyploids of D. alata would have appeared through the formation of unreduced gametes. The triploid pool would have been built and diversified through the formation of 2n gametes in diploid females as the result of the non-viability of seeds resulting from the formation of 2n sperm and of the non-viability of intercytotype crosses. The tetraploids would have appeared through bilateral sexual polyploidization via the union of two unreduced gametes due to the sterility of triploids.

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Section: Methodsmentioning

confidence: 99%

Microsatellite and flow cytometry analysis to help understand the origin of Dioscorea alata polyploids

Némorin

David²,

Maledon

et al. 2013

Annals of Botany

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“…Total genomic DNA was extracted from 100 to 200 mg of leaves using the CTAB method of Murray and Thompson (1980). We performed microsatellite amplification using the PCR following the methods of Mizuki et al (2005c). Of the 10 primer pairs for SSR loci that have been characterized for D. japonica (Mizuki et al 2005c), nine were used to identify the genets: DIJ0011, DIJ0012, DIJ0142, DIJ0206, DIJ0222, DIJ0342, DIJ0443, DIJ0461, and DIJ1045.…”

Section: Sampling and Ssr Analysismentioning

confidence: 99%

“…We performed microsatellite amplification using the PCR following the methods of Mizuki et al (2005c). Of the 10 primer pairs for SSR loci that have been characterized for D. japonica (Mizuki et al 2005c), nine were used to identify the genets: DIJ0011, DIJ0012, DIJ0142, DIJ0206, DIJ0222, DIJ0342, DIJ0443, DIJ0461, and DIJ1045. Because null alleles were suspected at two loci (DIJ0011, DIJ1045), we used primer pairs for the seven remaining loci for our analysis of the genetic structure.…”

Section: Sampling and Ssr Analysismentioning

confidence: 99%

Fine-scale spatial structure of genets and sexes in the dioecious plant Dioscorea japonica, which disperses by both bulbils and seeds

et al. 2010

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To understand the evolution of clonal reproduction and the diversity of clonal plants, it is necessary to clarify the characteristics of each clonal habit. There has been little research on whether bulbils alter spatial genetic structure (SGS) because of the lack of connection to maternal ramets. We used simple-sequence-repeat (SSR) markers to determine the fine-scale SGS of the dioecious plant Dioscorea japonica, which disperses both as bulbils and as seeds. We also evaluated the contributions of sexual and clonal reproduction and tested for spatial sex segregation (SSS). We discovered 111 genets from 394 ramets in a 2.8-ha plot. Genotypic richness (R = 0.28) and clonal diversity (Simpson's D = 0.94, Fager's E = 0.90) were high. We did not find SSS, suggesting that the population does not suffer from a shortage of mating pairs due to clonal reproduction. The Sp values revealed moderate SGS at the genet level (Sp = 0.013-0.014), and the genets intermingled at a local scale. Significant SGS at the ramet level showed that ramets within the same genet tended to aggregate. We also found a skewed clonal spatial distribution. The spatial extent of genets was positively correlated with the number of ramets within a genet. The contribution of bulbil production to the variance of parent-offspring gene dispersal was about one-fifth the contribution from sexual reproduction. These results suggest that the dispersal via bulbils affects the SGS in D. japonica, although its contribution to gene dispersal is small compared to the contribution of sexual reproduction.

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“…In addition, a joint CIRAD-INRA project is developing new microsatellites for linkage purposes. To date, only about 60 SSR markers developed from seven different yam species, D. tokoro (Terauchi and Konuma, 1994), D. rotundata ), D. alata, D. abyssinica, D. praehensilis (Tostain et al, 2006, D. japonica (Misuki et al, 2005), and D. trifida (Hochu et al, 2006), are available in yams.…”

Section: Molecular Markersmentioning

confidence: 99%

Root and Tuber Crops

Bradshaw¹

2010

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Development and characterization of microsatellite markers in a clonal plant, Dioscorea japonica Thunb.

Cited by 16 publications

References 11 publications

Microsatellite and flow cytometry analysis to help understand the origin of Dioscorea alata polyploids

Microsatellite and flow cytometry analysis to help understand the origin of Dioscorea alata polyploids

Fine-scale spatial structure of genets and sexes in the dioecious plant Dioscorea japonica, which disperses by both bulbils and seeds

Root and Tuber Crops

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