Development and characterization of hybrid tubular structure of PLCL porous scaffold with hMSCs/ECs cell sheet

Abstract

Tissue engineering offers an alternate approach to providing vascular graft with potential to grow similar with native tissue by seeding autologous cells into biodegradable scaffold. In this study, we developed a combining technique by layering a sheet of cells onto a porous tubular scaffold. The cell sheet prepared from co-culturing human mesenchymal stem cells (hMSCs) and endothelial cells (ECs) were able to infiltrate through porous structure of the tubular poly (lactide-co-caprolactone) (PLCL) scaffold and further proliferated on luminal wall within a week of culture. Moreover, the co-culture cell sheet within the tubular scaffold has demonstrated a faster proliferation rate than the monoculture cell sheet composed of MSCs only. We also found that the co-culture cell sheet expressed a strong angiogenic marker, including vascular endothelial growth factor (VEGF) and its receptor (VEGFR), as compared with the monoculture cell sheet within 2 weeks of culture, indicating that the co-culture system could induce differentiation into endothelial cell lineage. This combined technique would provide cellularization and maturation of vascular construct in relatively short period with a strong expression of angiogenic properties.

Porous biomaterials for tissue engineering: a review

“…The cells infiltrated the structure and populated the luminal wall, differentiating into angiogenic lineages. 454 Similarly, PCL/ Nylon 6 dual was electrospun to create porous tubular structures that positively influenced the cell morphology (fibrous like), attachment and proliferation of endothelial cells. 455 Fig.…”

Current Strategies for the Manufacture of Small Size Tissue Engineering Vascular Grafts

“…A three-layered TEVG has been fabricated overlapping nanofibers of PCL, collagen, and PLLA (Haghjooy Javanmard et al, 2016 ). PCL has also been used blended with collagen (Tillman et al, 2009 ; Bertram et al, 2017 ), gelatin (Jiang et al, 2017 ) and elastin (Wise et al, 2011 ) to improve surface adhesion features, while a PLCL porous scaffold was coated with nanofibers of silk fibroin (Henry et al, 2017 ) or alternatively with a layer of hMSCs/ECs (Ahn et al, 2015 ; Pangesty et al, 2017 ).…”

Design, Preparation, and Performance of a Novel Bilayer Tissue‐Engineered Small‐Diameter Vascular Graft

“…Pangesty et al. developed a combining technique through layering a sheet of cells onto a PLCL porous tubular scaffold, which provided cellularization and maturation of vascular construct in a relatively short period of time . A “cell matrix engineering” consisted of cells onto the PLCL electrospun sheet, and then the tubular structure was formed by rolling, which has a great promise for clinical applications…”