Development and characterization of an electrospun mat from Eri silk fibroin and PLA blends for wound dressing application

Abstract

Research is being carried out worldwide on the development of multilayer wound dressing systems with each layer satisfying the requirements of an ideal wound dressing system for achieving better wound healing performance. In this paper, mats were prepared from Eri silk fibroin (ESF) and its blend with poly-L-lactic acid (PLA) polymer by the electrospinning process and evaluated for the properties required for the skin contact layer of wound dressing systems. Tetracycline hydrochloride (TCH) was added to the polymer solution and was spun into a mat. The ESF fibres had a mean diameter of 320 nm and ESF, PLA blend (ESF-PLA) fibres had 502 nm. The phosphate buffer saline (PBS) uptake was less for the ESF-PLA mat compared to that of the 100% ESF mat. The contact angle with water was 23 for the ESF mat and 79 for the ESF-PLA mat. The vapour transmission rate was lower for the ESF-PLA mat compared to that of the 100% ESF mat, however, they were found to be lower than the level at which dehydration would occur. TCH loaded mats showed an effective zone of inhibition against gram positive and gram negative bacteria. A cytotoxicity study on neuroblast cells showed that the ESF mat did not impair the growth of cells. The in vivo study on a rat model showed that the wound healing performance of the mats developed from 100% ESF and ESF-PLA were better compared to the conventional open wound and gauze cloth wound dressing systems.

Hydroentangled nonwoven eri silk fibroin scaffold for tissue engineering applications

“…It has an hydrophilic to hydrophobic amino acid ratio of 0.35 [7]. In the recent years, eri silk has been studied extensively as a biomaterial for applications such as wound dressing, drug delivery, and scaffold for tissue engineering [8,9]. A number of methods, such as freeze-drying, salt leaching, gas forming and electrospinning, have been used to prepare silk scaffolds with various conformations and microstructures [10].…”

Borrowing From Nature: Biopolymers and Biocomposites as Smart Wound Care Materials

“…In the last decade, electrospun fibroin has been extensively proposed for the design of anti-bacterial, anti-inflammatory and anti-oxidant patches (Lin et al, 2016 ; Selvaraj and Fathima, 2017 ; Yang et al, 2017 ). To ease a water-based electrospinning process, silk has been processed either in combination with natural polymers, such as cellulose (Guzman-Puyol et al, 2016 ; Figures 2c,d ), gelatin (Shan et al, 2015 ), sericin (Hang et al, 2012 ), chitosan (Cai et al, 2010 ), alginate (Roh et al, 2006 ), elastin (Zhu et al, 2016 ), and hyaluronic acid (Yan et al, 2013 ), or mixed with synthetic materials, such as polyethylene oxide (Schneider et al, 2009 ; Wharram et al, 2010 ; Chutipakdeevong et al, 2013 ), polyvinyl alcohol (PVA; Chouhan et al, 2017 ), and poly-hydroxy esters (Lian et al, 2014 ; Shahverdi et al, 2014 ; Suganya et al, 2014 ; Shanmugam and Sundaramoorthy, 2015 ). Silk/PVA mats loaded with Ciprofloxacin and epidermal growth factors (Chouhan et al, 2017 ) enhanced human dermal fibroblasts and keratinocytes proliferation in vitro , and favored re-epithelization, mature collagen deposition and complete wound closure at 14 days in a in vivo wound healing rabbit model.…”

Novel Nonreleasing Antibacterial Hydrogel Dressing by a One-Pot Method

“…In recent years, a variety of wound dressings have been studied, including membranes, ,,, coatings, , mats, , electrospun nanofibers, , and hydrogels. ,− Among them, the hydrogel is widely reported for its high water content, easy removal, and biocompatibility. − Furthermore, the hydrogel has a wide range of biomedical applications, including drug-delivery systems, ,, tissue-engineering scaffolds, , and other medical devices. , In this study, a novel nonreleasing antibacterial hydrogel material with good protein adsorption performance, repeatable adhesiveness, and excellent mechanical performance was prepared by a one-step method, and the chemical structure, morphology feature, mechanical properties, and antibacterial performance of the hydrogel were systematically evaluated. The results of wound closure and histopathological examination on the full-thickness skin defect model of rats showed that this antibacterial hydrogel dressing could effectively promote wound healing.…”

“…As the human body’s largest organ, the skin is defenseless to a variety of damage, including burns, trauma, and chronic ulcers, which can injure the anatomy and function of the skin. , For deep dermic wounds, the healing process cannot happen spontaneously and should be supported by wound dressings, which are widely used to cure different types of skin injuries . The wound healing process is complex and dynamic.…”