Development and characterization of an enzyme-based lactate probe for undiluted media

Pfeiffer, D.; Setz, K.; Schulmeister, Thomas; Scheller, Frieder W.; Lück, H.; Pfeiffer, Dietrich

doi:10.1016/0956-5663(92)85024-5

Cited by 31 publications

(5 citation statements)

References 8 publications

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“…Therefore, this self-powered electrochemical lactate biosensor is calibrated to sense a wide range of lactate concentration from 1 to 100 mM to account for both diluted and undiluted samples, thereby confirming the utility of the system for sensing a wide range of lactic acid concentrations. This includes lactic acid concentrations that are at the normal lactate level in the human body to the maximum level that can occur [27,28]. Interfering analyte, such as uric acid is reported in a corresponding article to exhibit no impact of the sensing of the analyte of interest [15] using this self-powered lactate biosensor.…”

Section: Discussionmentioning

confidence: 99%

Self-Powered Electrochemical Lactate Biosensing

Baingane

Slaughter

2017

Energies

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This work presents the development and characterization of a self-powered electrochemical lactate biosensor for real-time monitoring of lactic acid. The bioanode and biocathode were modified with D-lactate dehydrogenase (D-LDH) and bilirubin oxidase (BOD), respectively, to facilitate the oxidation and reduction of lactic acid and molecular oxygen. The bioelectrodes were arranged in a parallel configuration to construct the biofuel cell. This biofuel cell's current-voltage characteristic was analyzed in the presence of various lactic acid concentrations over a range of 1-25 mM. An open circuit voltage of 395.3 mV and a short circuit current density of 418.8 µA/cm 2 were obtained when operating in 25 mM lactic acid. Additionally, a 10 pF capacitor was integrated via a charge pump circuit to the biofuel cell to realize the self-powered lactate biosensor with a footprint of 1.4 cm × 2 cm. The charge pump enabled the boosting of the biofuel cell voltage in bursts of 1.2-1.8 V via the capacitor. By observing the burst frequency of a 10 pF capacitor, the exact concentration of lactic acid was deduced. As a self-powered lactate sensor, a linear dynamic range of 1-100 mM lactic acid was observed under physiologic conditions (37 • C, pH 7.4) and the sensor exhibited an excellent sensitivity of 125.88 Hz/mM-cm 2 . This electrochemical lactate biosensor has the potential to be used for the real-time monitoring of lactic acid level in biological fluids.

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Section: Discussionmentioning

confidence: 99%

Self-Powered Electrochemical Lactate Biosensing

Baingane

Slaughter

2017

Energies

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“…From a practical point of view two main groups of multiple-use biosensors have been commercialised, membrane-based sensors from YSI [9,10], Eppendorf [11], Nova Biomedical [12, 13,14] and Radiometer [15], as well as thick film biosensors from Bayer [16] or Roche [17]. Membrane-based biosensors have an advantage in fabrication by using prefabricated membrane materials with well known properties, but the necessary membrane change is sometimes rather cumbersome.…”

Section: Introductionmentioning

confidence: 99%

Thick film biosensors for metabolites in undiluted whole blood and plasma samples

Schaffar

2002

Analytical and Bioanalytical Chemistry

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The new electrochemical thick film biosensors from Roche Diagnostics are presented. Following considerations about the principal requirements that biosensors have to fulfil to be useful for diagnostic purposes, the basic design of these thick film biosensors is shown. In this paper, the new generation of biosensors for glucose, lactate and urea are presented, as well as data from a new biosensor for creatinine. All biosensors are designed for multiple use, at minimum 500 samples or 1 week in-use (depending on type of enzyme used), for determinations in undiluted whole blood or plasma, with extra electrodes to compensate for interferences. The sensors are integrated in a disposable cassette requiring 38 microtl sample volume. The analytical ranges of the sensors scope well with the normal and pathological concentrations of metabolites in human blood, e.g. for glucose 0.5-40.0 mmol/L. Both biosensors and interference-compensating electrodes are developed to have a cycle time of 90 s maximum. Method comparison diagrams show excellent correlation of results obtained by biosensors compared to results achieved by reference methods. In addition, the possibility of urea and creatinine determinations in diluted urine is presented.

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“…Clark and Lyons (1962) developed the first glucose sensor, and much work has been reported on new types of glucose sensors (Bartlett et al, 1991;Cass et al, 1984;Mann-Buxbaum et al, 1990;. L-Lactate sensors have been developed for food analysis, fermentation control, and clinical diagnostics (Dremel et al, 1990;Kulys et al, 1993;Park et al, 1993;Pfeiffer et al, 1992). Bartlett and Caruana (1994) and Dempsey et al (1993) developed electropolymerization immobilization processes and studied characteristics of L-lactate sensors using flavocytochrome b 2 and lactate oxidase as biological components.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Food Application of an Amperometric Needle‐Type L‐Lactate Sensor

Kim

Haginoya

Karube

1996

Journal of Food Science

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There have been increasing needs for biosensing in the food industry because it is accurate, rapid and efficient. A needle-type L-lactate sensor with a three-layer membrane system which uses lactate oxidase as the biological component was prepared. The sensor had an optimum pH around 9-10 and an optimum temperature at 45ЊC. The current response was stable over 40 days and specifically responded to L-lactic acid. The sensor gave accurate L-lactate measurements in kimchi and yogurt of 187.4 ‫ע‬ 4.1 and 734.1 ‫ע‬ 34.5 mg/dL, similar to those of a spectrophotometric L-lactate kit.

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Development and characterization of an enzyme-based lactate probe for undiluted media

Cited by 31 publications

References 8 publications

Self-Powered Electrochemical Lactate Biosensing

Self-Powered Electrochemical Lactate Biosensing

Thick film biosensors for metabolites in undiluted whole blood and plasma samples

Characterization and Food Application of an Amperometric Needle‐Type L‐Lactate Sensor

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