Development and Characterization of Acellular Caprine Choncal Cartilage Matrix for Tissue Engineering Applications

Das, Piyali; Rajesh, Kanike; Lalzawmliana, V.; Devi, K. Bavya; Basak, Piyali; Lahiri, Debrupa; Kundu, Biswanath; Roy, Mousumi; Nandi, Samit Kumar

doi:10.1177/1947603519855769

Cited by 7 publications

(5 citation statements)

References 43 publications

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“…The development of acellular cartilage matrices while preserving the integrity and bioactivity of the ECM is the utmost prerequisite towards the utilization of acellular biomaterials for cartilage repair and regeneration. 185 In this context, Das et al 186 developed acellular cartilage xenogenic biomaterial from the caprine ear cartilage using two chemical treatments (Na-deoxycholate + SDS and GndHCl + NaOH). The decellularization process had no untoward effects on the ECM.…”

Section: Xenografts In Biomedical Applicationsmentioning

confidence: 99%

Waste-derived biomaterials as building blocks in the biomedical field

et al. 2022

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Section: Xenografts In Biomedical Applicationsmentioning

confidence: 99%

Waste-derived biomaterials as building blocks in the biomedical field

et al. 2022

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“…The major deciding factors during the selection of detergent for decellularization are (i) concentration at which it will not impair the ECM, (ii) the optimal perfusion rate, and (iii) duration for which the organ is being exposed to the detergent. Apart from these, the tissue (iv) thickness (v) lipid content, and (vi) cellular density is also responsible for the selection of decellularization detergent 75 . Using low detergent concentration at a high flow rate will not decellularize the tissue effectively while using detergent at a high concentration with a low flow rate will leave the ECM framework broken 76 .…”

Section: Discussionmentioning

confidence: 99%

“…Apart from these, the tissue (iv) thickness lipid content, and (vi) density is also responsible for the selection of decellularization detergent. 75 Using low detergent concentration at high flow rate will not decellularize the tissue effectively while using detergent at a high concentration with a low flow rate will leave the ECM framework broken. 76 The choice of detergent also has a major impact on after-effects on the ECM proteins, as a few studies have reported on detergents.…”

Section: Discussionmentioning

confidence: 99%

Establishment of decellularized extracellular matrix scaffold derived from caprine pancreas as a novel alternative template over porcine pancreatic scaffold for prospective biomedical application

et al. 2022

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In this study, the caprine pancreas has been presented as an alternative to the porcine organ for pancreatic xenotransplantation with lesser risk factors. The obtained caprine pancreas underwent a systematic cycle of detergent perfusion for decellularization. It was perfused using anionic (0.5% w/v sodium dodecyl sulfate) as well as non‐ionic (0.1% v/v triton X‐100, t‐octyl phenoxy polyethoxy ethanol) detergents and washed intermittently with 1XPBS supplemented with 0.1% v/v antibiotic and nucleases in a gravitation‐driven set‐up. After 48 h, a white decellularized pancreas was obtained, and its extracellular matrix (ECM) content was examined for scaffold‐like properties. The ECM content was assessed for removal of cellular content, and nuclear material was evaluated with temporal H&E staining. Quantified DNA was found to be present in a negligible amount in the resultant decellularized pancreas tissue (DPT), thus prohibiting it from triggering any immunogenicity. Collagen and fibronectin were confirmed to be preserved upon trichrome and immunohistochemical staining, respectively. SEM and AFM images reveal interconnected collagen fibril networks in the DPT, confirming that collagen was unaffected. sGAG was visualized using Prussian blue staining and quantified with DMMB assay, where DPT has effectively retained this ECM component. Uniaxial tensile analysis revealed that DPT possesses better elasticity than NPT (native pancreatic tissue). Physical parameters like tensile strength, stiffness, biodegradation, and swelling index were retained in the DPT with negligible loss. The cytocompatibility analysis of DPT has shown no cytotoxic effect for up to 72 h on normal insulin‐producing cells (MIN‐6) and cancerous glioblastoma (LN229) cells in vitro. The scaffold was recellularized using isolated mouse islets, which have established in vitro cell proliferation for up to 9 days. The scaffold received at the end of the decellularization cycle was found to be non‐toxic to the cells, retained biological and physical properties of the native ECM, suitable for recellularization, and can be used as a safer and better alternative as a transplantable organ from a xenogeneic source.

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“…Various types of detergents (for example ionic, non-ionic and zwitterionic detergents) are effective acellular chemical reagents. These detergents destroy the structure of cell membrane, separating DNA from proteins and remove cellular substances from the tissue ( Das et al, 2019 ; Das et al, 2020 ). The concentration and speed of action of the reagent will have an important impact on the following: its acellular effect, the structure and composition of the AC ECM, macrostructure destruction, decreases in the GAG content, and the change of micromechanical properties.…”

Section: Preparation Of the Ecm Scaffoldmentioning

confidence: 99%

Preparation and Application of Decellularized ECM-Based Biological Scaffolds for Articular Cartilage Repair: A Review

Zhang

Long

et al. 2022

Front. Bioeng. Biotechnol.

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Cartilage regeneration is dependent on cellular-extracellular matrix (ECM) interactions. Natural ECM plays a role in mechanical and chemical cell signaling and promotes stem cell recruitment, differentiation and tissue regeneration in the absence of biological additives, including growth factors and peptides. To date, traditional tissue engineering methods by using natural and synthetic materials have not been able to replicate the physiological structure (biochemical composition and biomechanical properties) of natural cartilage. Techniques facilitating the repair and/or regeneration of articular cartilage pose a significant challenge for orthopedic surgeons. Whereas, little progress has been made in this field. In recent years, with advances in medicine, biochemistry and materials science, to meet the regenerative requirements of the heterogeneous and layered structure of native articular cartilage (AC) tissue, a series of tissue engineering scaffolds based on ECM materials have been developed. These scaffolds mimic the versatility of the native ECM in function, composition and dynamic properties and some of which are designed to improve cartilage regeneration. This review systematically investigates the following: the characteristics of cartilage ECM, repair mechanisms, decellularization method, source of ECM, and various ECM-based cartilage repair methods. In addition, the future development of ECM-based biomaterials is hypothesized.

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Development and Characterization of Acellular Caprine Choncal Cartilage Matrix for Tissue Engineering Applications

Cited by 7 publications

References 43 publications

Waste-derived biomaterials as building blocks in the biomedical field

Waste-derived biomaterials as building blocks in the biomedical field

Establishment of decellularized extracellular matrix scaffold derived from caprine pancreas as a novel alternative template over porcine pancreatic scaffold for prospective biomedical application

Preparation and Application of Decellularized ECM-Based Biological Scaffolds for Articular Cartilage Repair: A Review

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