Development and characterization of 21 EST‐derived microsatellite markers in <i>Vicia faba</i> (fava bean)

Ma, Yue; Yang, Tao; Guan, Jianping; Wang, Shumin; Wang, Haifei; Sun, Xuelian; Zong, Xuxiao

doi:10.3732/ajb.1000407

Cited by 35 publications

(27 citation statements)

References 9 publications

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“…Ovesna et al [25] also reported that high number of allele was in accordance with high gene diversity in the study aiming to identify of Czech garlic varieties using 14 SSR markers. The number of allele and gene diversity in the present study coincided with Ma et al [26], who found the former between 2 and 9, and the latter between 0.0476 and 0.8304 in the study employing 32 accessions of V. faba and 21 microsatellite markers. Moreover, Gong et al [27] stated the number of allele varying from 1 to 3, and expected heterozygosity from 0 to 0.64 in the study Figure 1.…”

Section: Resultssupporting

confidence: 74%

Genetic diversity in some faba bean (Vicia faba L.) genotypes assessed by simple sequence repeats

Tufan

Erdoğan

2016

Biotechnology & Biotechnological Equipment

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The success of the breeding programmes depends on the dimension of genetic variability. Simple sequence repeats (SSR) have been extensively used in the studies including genetic characterization of many plant species. The objective of this study was to investigate the genetic variation in 22 faba bean genotypes, 18 of which were originated from International Center for Agricultural Research in the Dry Areas (ICARDA) and 4 of which were cultivated genotypes in Turkey, using SSR markers. Of 41 SSR markers used, 25 produced bands. As a result of SSR amplification, a total of 39 bands, 25 of which were polymorphic and 14 of which were monomorphic, were obtained. The mean gene diversity and polymorphism information content values were 0.27 and 0.24, respectively. The faba bean genotypes cultivated in Turkey had greater genetic diversity than those that originated from ICARDA. The faba bean genotypes FLIP10-and FLIP03-were successfully separated, using the un-weighted pair group method with arithmetic average dendrogram constructed via Jaccard similarity coefficients. These results were further supported by factor analysis substantially. The results indicated that there is sufficient genetic diversity among the tested faba bean genotypes (especially cultivated in Turkey) and could be used in faba bean breeding programmes.

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Section: Resultssupporting

confidence: 74%

Genetic diversity in some faba bean (Vicia faba L.) genotypes assessed by simple sequence repeats

Tufan

Erdoğan

2016

Biotechnology & Biotechnological Equipment

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“…Moreover, our data revealed that the number of alleles detectable in Tunisian faba bean populations is comparable with the number of alleles detected in traditional Korea population (Suresh et al 2013). On the other hand, the mean number of alleles was high (6.62) compared with the mean number of alleles per locus for China and some Europen (2.76) faba bean genotypes (Ma et al 2011). These data suggested that Tunisian faba bean populations are polymorphic, providing a large number of alleles within populations.…”

Section: Discussionmentioning

confidence: 75%

“…Eight simple sequence repeat (SSR) primer pairs were used for molecular analyses. Six primer pairs were obtained from Ma et al (2011): SSR3, SSR4, SSR5, SSR6, SSR7 and SSR8, and two primer pairs were obtained from Gong et al (2010): SSR1 and SSR2. The PCR reactions were carried out in a volume of 25 ll containing 50 ng genomic DNA, 0.3 lM forward and reverse primers, 2 mM MgCl 2 , 0.3 mM dNTPs, 1U Taq DNA polymerase (Thermo Fisher Scientific, Germany), in 1xPCR buffer (10 mM Tris-HCl, pH 8.3 and 50 mM KCl).…”

Section: Molecular Analysismentioning

confidence: 99%

Genetic variability in Tunisian populations of faba bean (Vicia faba L. var. major) assessed by morphological and SSR markers

Rebaa¹,

Abid²,

Aouida³

et al. 2017

Physiol Mol Biol Plants

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The genetic diversity of 21 faba bean populations was examined using morphological and molecular markers. DNA was extracted from 189 individuals and 8 microsatellite markers were genotyped individually in these 21 populations. A total of 53 alleles were obtained in all populations, with an average of 6.62 alleles per locus. The expected and observed heterozygosity was 0.38 and 0.62 respectively. The average polymorphism index content of SSR markers was 0.61, ranging from 0.31 to 0.81. The unweighted pair group method with arithmetic mean dendrogram clustered all the populations into two groups, each for them subdivided into 3 sub-groups according to geographical origin. Morphological variation showed that the populations were not grouped according to their geographical origin. Therefore, patterns of differentiation of morphological traits did not coincide with molecular differentiation, indicating that morphological variation does not reflect genetic subdivision in studied faba bean populations. Analysis of molecular variance revealed high levels of genetic variation (83%) within population and provides a good base for designing genetic improvement programs. The result of Principal Component Analysis (PCA) revealed that three dimensional principal components (PC1, PC2 and PC3) contributed 40.56% of the total variability and accounted with values of 20.64, 11.22 and 8.70%, respectively. Cluster analysis based on PCA indicated three separate groups of populations. The genetic relationships found between the 21 populations samples were the same in both the PCA and STRUCTURE analysis which support the results observed. These data may serve as a foundation for the development of faba bean breeding programs.

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“…Among them, 128 SSR markers were referred to in Ma et al [25], 236 SSR markers were selected from a linkage map developed by El-Rodeny et al [26], and the remaining SSR markers (unpublished data) were kindly provided by the Institute of Crop Science, Chinese Academy of Agricultural Sciences (ICP, CAAS). The ISSR markers used in this study were according to Zietkiewicz et al [27].…”

Section: Marker Analysismentioning

confidence: 99%

“…The SSR markers were firstly randomly selected from each linkage group (LG) according to Ma et al [25] and El-Rodeny et al [26]. Also subsequently, unpublished SSR markers (including EST sequences) were kindly provided by the Institute of Crop Science, Chinese Academy of Agricultural Sciences (ICP, CAAS).…”

Section: Ssr and Issr Markersmentioning

confidence: 99%

Linkage Map of a Gene Controlling Zero Tannins (zt-1) in Faba Bean (Vicia faba L.) with SSR and ISSR Markers

et al. 2018

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Faba bean (Vicia faba L.), a partially allogamous species, is rich in protein. Condensed tannins limit the use of faba beans as food and feed. Two recessive genes, zt-1 and zt-2, control the zero tannin content in faba bean and promote a white flower phenotype. To determine the inheritance and develop a linkage map for the zt-1 gene in the faba bean germplasm M3290, F 2 and F 3 progenies were derived from the purple flower and high tannin content genotypes Qinghai12 and zt-1 line M3290, respectively. Genetic analysis verified a single recessive gene for zero tannin content and flower colour. In total, 596 SSR markers and 100 ISSR markers were used to test the polymorphisms between the parents and bulks for the contrasting flower colour via Bulked Segregant Analysis (BSA). Subsequently, six SSR markers and seven ISSR markers were used to genotype the entire 413 F 2 population. Linkage analysis showed that the zt-1 gene was closely linked to the SSR markers SSR84 and M78, with genetic distances of 2.9 and 5.8 cM, respectively. The two flanked SSR markers were used to test 34 faba bean genotypes with different flower colours. The closely linked SSR marker SSR84 predicted the zt-1 genotypes with absolute accuracy. The results from the marker-assisted selection (MAS) from this study could provide a solid foundation for further faba bean breeding programmes.

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Development and characterization of 21 EST‐derived microsatellite markers in Vicia faba (fava bean)

Abstract: These markers will be useful to study genetic diversity, genetic mapping, and molecular breeding in fava bean.

Cited by 35 publications

References 9 publications

Genetic diversity in some faba bean (Vicia faba L.) genotypes assessed by simple sequence repeats

Genetic diversity in some faba bean (Vicia faba L.) genotypes assessed by simple sequence repeats

Genetic variability in Tunisian populations of faba bean (Vicia faba L. var. major) assessed by morphological and SSR markers

Linkage Map of a Gene Controlling Zero Tannins (zt-1) in Faba Bean (Vicia faba L.) with SSR and ISSR Markers

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