Linkage Map of a Gene Controlling Zero Tannins (zt-1) in Faba Bean (Vicia faba L.) with SSR and ISSR Markers

Abstract

Faba bean (Vicia faba L.), a partially allogamous species, is rich in protein. Condensed tannins limit the use of faba beans as food and feed. Two recessive genes, zt-1 and zt-2, control the zero tannin content in faba bean and promote a white flower phenotype. To determine the inheritance and develop a linkage map for the zt-1 gene in the faba bean germplasm M3290, F 2 and F 3 progenies were derived from the purple flower and high tannin content genotypes Qinghai12 and zt-1 line M3290, respectively. Genetic analysis verified a single recessive gene for zero tannin content and flower colour. In total, 596 SSR markers and 100 ISSR markers were used to test the polymorphisms between the parents and bulks for the contrasting flower colour via Bulked Segregant Analysis (BSA). Subsequently, six SSR markers and seven ISSR markers were used to genotype the entire 413 F 2 population. Linkage analysis showed that the zt-1 gene was closely linked to the SSR markers SSR84 and M78, with genetic distances of 2.9 and 5.8 cM, respectively. The two flanked SSR markers were used to test 34 faba bean genotypes with different flower colours. The closely linked SSR marker SSR84 predicted the zt-1 genotypes with absolute accuracy. The results from the marker-assisted selection (MAS) from this study could provide a solid foundation for further faba bean breeding programmes.

Construction of the first ultra-dense genetic map for faba bean (Vicia faba L.) and quantitative trait loci mapping for seed-related traits

“…also identi ed ve genes that control protein content in faba bean. Gutierrez et al (2007) identi ed two SCAR markers tightly linked to a gene controlling tannin de ciency in faba beans and Hou et al (2018) screened one SSR marker (SSR84) closely linked to the tannin content (zt-1) gene using 596 SSR markers and 100 ISSR markers, which could aid in accurate prediction of the zt-1 genotypes.…”

Development and validation of a robust KASP marker for zt2 locus in faba bean (Vicia faba)

“…Later, Webb et al () developed a SNP (single nucleotide polymorphism) marker for ZT1 using a set of SNP markers derived from the genome of the fully sequenced model legume species Medicago truncatula. Recently, Hou et al () reported a reliable SSR (simple sequence repeat) marker that predicted zt1 genotypes with high accuracy. A robust breeder‐friendly molecular marker for zt2 gene is not yet available for faba bean.…”

Bumblebee responses to variation in pollinator‐attracting traits of Vicia faba flowers

“…Initial experiments focused on lines NV641 and NV676, which were similar in morphology but differed in the absence/presence of spots and their floral VOCs. We were interested in spot presence as this trait is associated with the nutritional content of the crop (Hou et al., 2018), and we wanted to determine if this would lead to any trade‐offs with bee preference. Additional experiments explored more subtle variation in spot size, choosing the extremes of our dataset as bees were most likely to be able to discriminate between them.…”