Development and Application of the Novel Visual Loop-Mediated Isothermal Amplification of Omp25 Sequence for Rapid Detection of Brucella sp.

Pan, Wei; Shen, Hao; Zhao, Mingqiu; Ju, Chunmei; Dong, Xing; Yi, Lingling; Wang, J.Y.; Chen, J.D.

doi:10.3923/javaa.2011.2120.2126

Cited by 27 publications

(24 citation statements)

References 9 publications

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“…The LAMP method does not require a thermal cycler, making it suitable for on-site applications. Although this method has been applied to detect and identify bacteria (Pan et al 2011), viruses (Niessen and Vogel 2010), and some specialized forms and races of Fusarium (Almasi et al 2013;Li et al 2013;Peng et al 2013), few studies have applied the LAMP assay to rapidly and specifically detect F. equiseti or F. graminearum, the causative agents of Fusarium root rot in soybean.…”

Section: Introductionmentioning

confidence: 99%

Rapid diagnosis of Fusarium root rot in soybean caused by Fusarium equiseti or Fusarium graminearum using loop-mediated isothermal amplification (LAMP) assays

Zhang

Wang

et al. 2015

Australasian Plant Pathol.

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Rapid diagnostic assays for Fusarium root rot in soybean, caused by Fusarium equiseti or Fusarium graminearum were developed using the target gene CYP51C. Both assays amplified the target gene in 60 min at 62°C, after which they were assessed for specificity and sensitivity. Specificity was evaluated against other Fusarium spp., other fungal species, and oomycetes. A positive yellow-green color (by the naked eye) or intense green fluorescence (under ultraviolet light) was observed only in the presence of F. equiseti or F. graminearum after adding SYBR Green I, whereas other strains showed either no color change or weak fluorescence. The detection limit of the CYP51C-Fe-LAMP assay was 10 pg. μL −1 genomic DNA per reaction, and as few as four conidia per gram of soil could be detected; and the detection limit of the CYP51C-Fg-LAMP assay was 100 pg. μL −1 genomic DNA per reaction, and 40 conidia per gram of soil could be identified. The assays also detected F. equiseti or F. graminearum from inoculated soybean tissues and diseased plants in the field. These results suggest that CYP51C-Fe-LAMP assay and CYP51C-Fg-LAMP assay are effective in rapidly diagnosing soybean root rot caused by F. equiseti and F. graminearum.

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Section: Introductionmentioning

confidence: 99%

Rapid diagnosis of Fusarium root rot in soybean caused by Fusarium equiseti or Fusarium graminearum using loop-mediated isothermal amplification (LAMP) assays

Zhang

Wang

et al. 2015

Australasian Plant Pathol.

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“…LAMP for the Brucella was developed against Brucella cell surface protein (bcsp)31 gene and omp25 gene and sensitivity of both LAMP assay was higher than PCR (Ohtsuki et al, 2008;Lin et al, 2011). LAMP with visual detection based on calcein has also been developed targeting the same omp25 (Pan et al, 2011). Reports regarding development of LAMP targeting IS711 genes and real time quantitative LAMP are available (Pérez-Sancho et al, 2013).…”

Section: Serological Tests Like Rose Bengal Plate Test (Rbpt) Standamentioning

confidence: 99%

Equine brucellosis: Review on epidemiology, pathogenesis, clinical signs, prevention and control

Karthik¹,

Prabakar²,

Bharathi³

et al. 2016

JEBAS

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KEYWORDS Brucellosis Equine B. abortus Poll evil Fistulous withers LAMP ABSTRACTBrucellosis is one of the major zoonotic diseases that affect several domestic animals, wild animals and also marine mammals. Though there is no specific Brucella sp. that can affect horses, B. abortus and B. suis can affect horses naturally and B. canis experimental infection has also been reported in equines. Brucellosis in equines is characterized by two conditions namely Poll evil and fistulous withers. Organism has its predilection for joints, ligaments and tendons in case of equines and causes inflammatory conditions leading to formation of fistula. Equine brucellosis has been documented from several parts of the world and prevalence has been reported time to time mostly based on serological diagnosis. Diagnosis of brucellosis mainly depends on serological methods though isolation of the organism is the gold standard. Due to hazardous nature of the pathogen, tests like Rose Bengal plate agglutination test, Standard tube agglutination test and other serological assays are commonly employed. Isothermal amplification assay like LAMP are gaining momentum these years due to swiftness in diagnosis of the pathogen. LAMP with high specificity and sensitivity for detection of Brucella spp. and also B. abortus has been developed in the recent years. Prevention and control of brucellosis is of utmost important to halt the spread of the organism to other animals and human. Trauma is a major reason for predisposition of poll evil and fistulous withers hence proper fitting of saddle will help to prevent the disease. Housing and feeding the horses separately can prevent spread of disease from cattle. The present review discusses equine brucellosis, its epidemiology, pathogenesis, clinical signs along with appropriate prevention and control strategies to be adapted.

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“…The amplified products of LAMP could be visualised either by gel electrophoresis generating a ladder of DNA fragments or simply detected visualising fluorescence by adding fluorescent dyes to the reaction tube after amplification [7]. Till now only four reports of LAMP on Brucella have been published [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Development of the Novel Loop Mediated Isothermal Amplification (LAMP) of IS711 Sequence for Rapid Detection of Brucella Species

Zadon

Sharma

Arora

et al. 2014

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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A reliable and novel loop mediated isothermal amplification (LAMP) was developed in the laboratory for rapid and specific detection of the Brucella species. Four specific LAMP primers were designed targeting IS711 gene, a highly conserved element in the genus Brucella. The assay could correctly amplified Brucella abortus S19, B. abortus S99, B. abortus, B. melitensis and eight clinical isolates of B. abortus but did not show any cross reaction with non-Brucella organisms. Detection limit of LAMP assay was 75 fg.

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Development and Application of the Novel Visual Loop-Mediated Isothermal Amplification of Omp25 Sequence for Rapid Detection of Brucella sp.

Cited by 27 publications

References 9 publications

Rapid diagnosis of Fusarium root rot in soybean caused by Fusarium equiseti or Fusarium graminearum using loop-mediated isothermal amplification (LAMP) assays

Rapid diagnosis of Fusarium root rot in soybean caused by Fusarium equiseti or Fusarium graminearum using loop-mediated isothermal amplification (LAMP) assays

Equine brucellosis: Review on epidemiology, pathogenesis, clinical signs, prevention and control

Development of the Novel Loop Mediated Isothermal Amplification (LAMP) of IS711 Sequence for Rapid Detection of Brucella Species

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