Development and application of spectrophotometric method for quantitative determination of Metronidazole in pure and tablet formulations

Abstract

A rapid, simple and sensitive spectrophotometric method has been developed for the determination of metronidazole in pharmaceutical pure and dosage forms. The method depends on alkaline hydrolysis of metronidazole releases the nitro group as nitrite ion and yielded nitrite ions can be used to give a colored complex that absorbs maximally at 505 nm. Beer’s law is obeyed in the concentration ranges 9-100 mg/ml with molar absorptivity of 1.14 ×103 L mole-1 cm-1. The proposed method is precise, accurate and specific for the quantitative determination of drug in bulk and dosage forms. The results of analysis of commercial formulations and the recovery study of metronidazole suggested that there is no interference from any excipients, which are present in pharmaceutical formulations of metronidazole. Statistical comparison of the results was performed with regard to accuracy and precision using student’s t-test and F-ratio at 95% confidence level. There is no significant difference between the reported and proposed methods with regard to accuracy and precision.

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“…One known concentration to three unknown concentrations of samples containing different concentrations of each was added purpose for done the initial calibration and the predictive ability of the updated sample was checked using external validation samples, then calculate the perform sample updating for each component using the developed method RP-HPLC with three concentrations of the added updating samples. [33][34][35][36]…”

Formulation and Optimization of Immediate Release Cajanus Cajan Starch-Based Tablets Containing Metronidazole

“…Also, few researchers had declared that Cajanus cajan contains high carbohydrate sources, making it a prospective origin for starch. 5 Metronidazole is a nitroimidazole compound, 10 used widely as an antibiotic against anaerobes, 11 and for treatment of infection caused by protozoa. 12 The MNA powder possesses poor compression characteristics.…”

Green versatile micellar electrokinetic chromatographic method for determination of six antimicrobial and anti-inflammatory drugs in combined dosage forms

“…Regarding DXM, the recent methods include spectrophotometric [17], TLC [18], HPLC [19,20] and CE [21] methods. For MNZ, the reported methods include spectrophotometric [22,23] electrochemical [24,25], TLC [26,27] HPLC [28,29] and CE [30,31] methods. Determination of ORZ was carried out using spectrophotometric [32,33] and HPLC [34,35] methods.…”