Development and application of RAPD-SCAR markers to identify intra-species hybrids of industrial Saccharomyces cerevisiae

Wang, Pin-Mei; Wu, Ximei; Chi, Xiaoqin; Li, Yudong; Zheng, Dao-Qiong; Ding, Rui; Min, Hang

doi:10.1007/s11274-010-0430-7

Cited by 7 publications

(4 citation statements)

References 11 publications

(13 reference statements)

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“…R. solanacearum [20], R. solani [21], F. oxysporum f. sp. radicis-lycopersici [22], Saccharomyces cerevisiae [6] and Trichoderma harzanium Trichoderma viride [23] etc.…”

Section: Sequence Characterized Amplified Regions (Scar)mentioning

confidence: 99%

“…The strains of Saccharomyces cerevisiae, the most useful species of yeast, are instrumental to winemaking, baking, and brewing since ancient times. Protoplast fusion has the potential to generate improved hybrids possessing novel combinations of parent characteristics [6]. Yeast breeding by protoplast fusion however, has a limited practical applications that can be successfully employed for screening of hybrid strains [26].…”

Section: Applications Of Scar Markermentioning

confidence: 99%

“…The parent Saccharomyces cerevisiae strains Z8 and Z9, showing high ethanol production, were electrofused and hybrids were screened by PCR using SCAR markers. Hence utility of SCAR markers can be employed in selecting strains from protoplast fusion in industrial breeding [6].…”

Section: Applications Of Scar Markermentioning

confidence: 99%

“…SCAR markers have proven their utility in detection of Fusarium oxysporium f. sp. ciceris and cubense [4] [5], selection of hybrid strains of Saccharomyces cerevisiae [6], validation of sex of plants in early stage [7] and identification of powdery mildew resistance genes [8]. The several potential applications that are put together by earlier workers are well summarized in Table 1.…”

Section: Introductionmentioning

confidence: 99%

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RAPD-SCAR Markers: An Interface Tool for Authentication of Traits

Bhagyawant¹

2016

JBM

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The versatility of the PCR technique is that several kinds of primers can be explored for genome analysis depending on the purpose of study. The easy to access and low cost PCR-based markers include Random Amplified Polymorphic DNA (RAPD). The RAPD markers are easy to develop but lack of reproducibility makes it less reliable and obstacles to their further use in authentication of traits. In addition, other PCR and non PCR based markers like Amplified Fragment Length Polymorphism (AFLP), Simple Sequence Repeat (SSR) and Restriction Fragment Length Polymorphism (RFLP) are also employed in authentication of traits with certain restrictions vis-à-vis use of radioactive materials, high cost and requirement of sequence information etc. Therefore, this problem can be overcome by converting RAPD markers to more robust sequence characterized amplified regions i.e. SCAR markers. SCARs are locus specific, co-dominant in nature and amplified by PCR using specific 15-30 bp DNA fragments. For developing SCAR markers, primers are designed from the nucleotide sequences of a cloned RAPD fragments linked to a trait of interest. SCAR markers are easy to develop and reliable tools for DNA fingerprinting. This mini review is an attempt to summarize efficacy of RAPD-SCAR interface in authentication of traits.

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“…R. solanacearum [20], R. solani [21], F. oxysporum f. sp. radicis-lycopersici [22], Saccharomyces cerevisiae [6] and Trichoderma harzanium Trichoderma viride [23] etc.…”

Section: Sequence Characterized Amplified Regions (Scar)mentioning

confidence: 99%

Section: Applications Of Scar Markermentioning

confidence: 99%

Section: Applications Of Scar Markermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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RAPD-SCAR Markers: An Interface Tool for Authentication of Traits

Bhagyawant¹

2016

JBM

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Development of a novel SRAP-SCAR marker for rapid identification of lager and ale types in brewer’s yeast

Liu

Cai

et al. 2022

Mol Biol Rep

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Development of species-specific ISSR-derived SCAR marker for early discrimination between Cinnamomum verum and Cinnamomum cassia

Gangwar¹,

Gahlaut²,

Chauhan³

et al. 2023

Mol Biol Rep

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Background Cinnamomum verum (true cinnamon) and Cinnamomum cassia (cassia cinnamon) are two important species belonging to family Lauraceae. These species are recognized by morphological, chemical composition and essential oil contents. The appropriate identi cation of species would be considerably improved by a genetic method. The main objective of the present study was to develop molecular markers distinguishing between C. verum and C. cassia.Methods and results A total 71 ISSR (Inter simple sequence repeat) and four universal barcoding (ITS, rbcL, matK, and psbA-trnH)genes were used to distinguish both the species. No sequence variation was observed between the two species for any DNA barcode gene. However, one ISSR i.e. ISSR-37 showed a clear distinction between the species and produced 570 bp and 746 bp amplicons in C. verum and C. cassia, respectively. The polymorphic bands were converted into species speci c SCAR markers. The SCAR-CV was speci c to C. verum and ampli ed 190 bp band, however there was no ampli cation seen in the C. cassia samples. ConclusionThe SCAR primers generated in this study can be employed as e cient, economical, and reliable molecular markers for the identi cation of C. verum.

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Development and application of RAPD-SCAR markers to identify intra-species hybrids of industrial Saccharomyces cerevisiae

Cited by 7 publications

References 11 publications

RAPD-SCAR Markers: An Interface Tool for Authentication of Traits

RAPD-SCAR Markers: An Interface Tool for Authentication of Traits

Development of a novel SRAP-SCAR marker for rapid identification of lager and ale types in brewer’s yeast

Development of species-specific ISSR-derived SCAR marker for early discrimination between Cinnamomum verum and Cinnamomum cassia

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