Development and application of a new on-line SPE system combined with LC–MS/MS detection for high throughput direct analysis of pharmaceutical compounds in plasma

Alnouti, Yazen; Srinivasan, Karthik; Waddell, David; Bi, Honggang; Kavetskaia, Olga; Gusev, Arkady I.

doi:10.1016/j.chroma.2005.04.056

Cited by 98 publications

(51 citation statements)

References 33 publications

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“…[38][39][40]44 This is critical for some applications such as pharmacokinetic studies that handle a large number of samples. Theron et al 44 described a method for quantification of EFZ in human saliva with a LLOQ of 6.11 ng mL -1 , but with a long analysis time 8.5 min.…”

Section: Discussionmentioning

confidence: 99%

A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study

Bedor¹,

Filho²,

Ramos³

et al. 2011

Quím. Nova

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Recebido em 12/7/10; aceito em 17/12/10; publicado na web em 25/3/11An LC-MS/MS method has been developed for the determination of efavirenz (EFZ) in human plasma using hydrochlorothiazide as internal standard (I.S.). An ESI negative mode with multiple reaction-monitoring was used monitoring the transitions m/z 313.88→69.24 (EFZ) and 296.02→204.76 (I.S.). Samples were extracted using liquid-liquid extraction. The total run time was 2.0 min. The separation was achieved with HPLC-RP using a monolithic column. The assay was linear in the concentration range of 100 -5000 ng mL -1 . The mean recovery was 83%. Intra-and inter-day precision were < 9.5% and < 8.9%, respectively and accuracy was in the range ± 8.33%. The method was successfully applied to a bioequivalence study.

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Section: Discussionmentioning

confidence: 99%

A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study

Bedor¹,

Filho²,

Ramos³

et al. 2011

Quím. Nova

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show abstract

“…The lower limit of quantitation was 0.1 ng/mL and the linear calibration range was from 0.1 to 100 ng/mL for both analytes. A direct coupling of a pre-analytics module directly with a LC-MS/MS instrument is feasible as well, either by a dedicated system (Alnouti et al, 2005;Koal et al, 2006) or by implementing a HPLC injection valve onto a generic liquid handling system (Daurel-Receveur et al, 2006). A 96-well LLE LC-MS/MS method for determination of a basic and polar drug candidate from plasma samples developed (Xu et al, 2004).…”

Section: Automated Off-line Sample Preparationmentioning

confidence: 99%

“…This new technique was applied for direct analysis of model compounds in rat plasma using on-line SPE-LC-MS/MS quantification. On-line IS introduction allows for non-volumetric sample (plasma) collection and direct analysis without the need of measuring and aliquoting a fixed sample volume prior to the on-line SPE-LC-MS/MS that enables direct sample (plasma) analysis without any sample manipulation and preparation (Koal et al, 2006;Alnouti et al, 2005). On-line SPE with high flow rate (normally, 4~6 mL/min) has been achieved by using extraction columns packed with large diameter particles.…”

Section: On-line Solid-phase Extraction (Spe)mentioning

confidence: 99%

Principles and Applications of LC-MS/MS for the Quantitative Bioanalysis of Analytes in Various Biological Samples

Kang¹

2012

Tandem Mass Spectrometry - Applications and Principles

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“…16 Hysphere C18 HD SPE stationary phase was chosen for sample preparation purpose, allowing higher load capacity and more uniform analyte extraction reproducibility. [24][25][26][27][28] After mobile phase desorbed analytes and Vol. 40, No.…”

Section: On-line Spe Processmentioning

confidence: 99%

On-line solid phase extraction coupled with liquid chromatography tandem mass spectrometry for the determination of codeine in human plasma

Cruz

Suenaga

Abib³

et al. 2016

Quim. Nova

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publicado na web em 26/08/2016A specific LC-MS/MS method was developed and validated for automated determination of codeine in human plasma, using online solid phase extraction (SPE) system coupled with positive ion electrospray ionization tandem mass spectrometry. The method allowed plasma direct injection onto cartridge without sample pre-treatment. Total analysis time per run was 3 min, allowing highthroughput for codeine determination. SPE on-line along a monolithic column (Chromolith Performance RP-18e, 100 mm x 4.6 mm) demonstrated to be highly effective in terms of backpressure, separation speed and peak asymmetry. Calibration curves range was linear 5.0-200 ng mL -1 . Method showed an excellent intra-day and inter-day precision ranged from 2.34 to 7.25% (CV%) as well as great intra-day and inter-day accuracy, ranging from 97.64 to 110% (RE%). SPE-LC-MS/MS method provided selectivity, accuracy, precision, fastness and high-throughput to assess codeine pharmacokinetics in human plasma samples.

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Development and application of a new on-line SPE system combined with LC–MS/MS detection for high throughput direct analysis of pharmaceutical compounds in plasma

Cited by 98 publications

References 33 publications

A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study

A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study

Principles and Applications of LC-MS/MS for the Quantitative Bioanalysis of Analytes in Various Biological Samples

On-line solid phase extraction coupled with liquid chromatography tandem mass spectrometry for the determination of codeine in human plasma

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