Development and application of a visual loop-mediated isothermal amplification combined with lateral flow dipstick (LAMP-LFD) method for rapid detection of Salmonella strains in food samples

Mei, Xueran; Zhai, Xiaoyu; Lei, Changwei; Ye, Xiaolan; Kang, Zhuang-Zhuang; Wu, Xuan; Xiang, Rong; Wang, Yulong; Wang, Hongning

doi:10.1016/j.foodcont.2019.04.014

Cited by 66 publications

(36 citation statements)

References 78 publications

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“…Recombinase polymerase amplification could amplify target fragments in a shorter time (10 min). By contrast, a longer time (40 min) is required for a Loop-mediated isothermal amplification (LAMP) assay, and approximately 40 cycles (40 min) are required in the qPCR assay [1,13,28]. Clearly, the RPA assay is more efficient.…”

Section: Discussionmentioning

confidence: 99%

Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow Immunoassay for Rapid Detection of Salmonella in Food

Fang

et al. 2019

Foods

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Salmonella can cause serious foodborne diseases. We have developed a lateral flow immunoassay combined with recombinase polymerase amplification (LFD-RPA) for detection of Salmonella in food. The conserved fragment (fimY) was selected as the target gene. Under an optimal condition (37 °C, 10 min), the sensitivity was 12 colony-forming units (CFU)/mL in a pure culture. Testing with 16 non-Salmonella strains as controls revealed that LFD-RPA was specific to the fimY gene of Salmonella. The established assay could detect Salmonella at concentrations as low as 1.29 × 102 CFU/mL in artificially contaminated samples. This detection was at a slightly higher level than that for a pure bacterial culture. Combined with the test strip reader, the LFD-RPA is a feasible method for quantitative detection of Salmonella based on the test line intensity, which was the ratio for the test line and control line of the reflected light. The method could be a potential point-of-care test in limited resource areas and provides a new approach and technical support for the diagnosis of food safety.

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Section: Discussionmentioning

confidence: 99%

Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow Immunoassay for Rapid Detection of Salmonella in Food

Fang

et al. 2019

Foods

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“…Several LFAs compatible with LAMP have been developed to allow for the detection of foodborne pathogens in various food matrices, including meats, produce, milk, and seafood. [95][96][97][98] A number of studies have attempted to integrate the LFA with more PON sample processing steps. 99,100 For example, the Kim group utilized a cellulose nitrate filter to concentrate and isolate E. coli in beef samples before downstream LAMP and LFA detection.…”

Section: Paper-based Detection Methodsmentioning

confidence: 99%

Point-of-Need Diagnostics for Foodborne Pathogen Screening

et al. 2021

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“…Many previous reports published only a single LFB assay for the detection of either Campylobacter or Salmonella spp. in food [37][38][39], as it is challenging to enrich both microorganisms simultaneously, due to the fact that their growth requirements on different selective broths as well as slower growth of Campylobacter under microaerobic conditions [40]. However, as previously described [41], both organisms contaminated in food were enriched independently and were then gathered for DNA extraction.…”

Section: And S1 Table) As Well As In Allmentioning

confidence: 99%

“…However, the analytical sensitivity of our d-LAMP-LFB assay in spiking study was also comparable to the LFB in other previous studies. It has been reported that the lowest inoculated detection limit for Salmonella targeting invA gene was 8×10 3 CFU/25 g (320 CFU/g) after enrichment for 18-24 h [37], 36 CFU/25 g (1.44 CFU/g) targeting hilA gene after 6 of enrichment [38], while C. jejuni was 10 1 −10 2 CFU/ 25 g (or 10 1 −10 2 CFU/mL of initial inoculum levels) targeting hipO gene without enrichment step [39]. Several qPCR kits such as BAX 1 Campylobacter coli/jejuni/lari assay (DuPont Qualicon), BAX 1 Salmonella assay (Hygiena), BIOTECON foodproof 1 Campylobacter Detection Kit (Biotecon Diagnostics) and BIOTECON foodproof 1 Salmonella Detection Kit (Biotecon Diagnostics) are commercially available with the level of detection as low as 1-10 CFU/25 g sample or 10 3 −10 4 CFU/mL after 20-24 h and 24-48 h of enrichment for Salmonella and Campylobacter, respectively.…”

Section: Plos Onementioning

confidence: 99%

Rapid and simultaneous detection of Campylobacter spp. and Salmonella spp. in chicken samples by duplex loop-mediated isothermal amplification coupled with a lateral flow biosensor assay

et al. 2021

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Development of a simple, rapid and specific assay for the simultaneous detection of Campylobacter spp. and Salmonella spp. based on duplex loop-mediated isothermal amplification (d-LAMP), combined with lateral-flow biosensor (LFB) is reported herein. LAMP amplicons of both pathogens were simultaneously amplified and specifically differentiated by LFB. The specificity of the d-LAMP-LFB was evaluated using a set of 68 target and 12 non-target strains, showing 100% inclusivity and exclusivity. The assay can simultaneously detect Campylobacter and Salmonella strains as low as 1 ng and 100 pg genomic DNA per reaction, respectively. The lowest inoculated detection limits for Campylobacter and Salmonella species in artificially contaminated chicken meat samples were 103 CFU and 1 CFU per 25 grams, respectively, after enrichment for 24 h. Furthermore, compared to culture-based methods using field chicken meat samples, the sensitivity, specificity and accuracy of d-LAMP- LFB were 95.6% (95% CI, 78.0%-99.8%), 71.4% (95% CI, 29.0%-96.3%) and 90.0% (95% CI, 73.4%-97.8%), respectively. The developed d-LAMP-LFB assay herein shows great potentials for the simultaneous detection of the Campylobacter and Salmonella spp. and poses a promising alternative approach for detection of both pathogens with applications in food products.

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Development and application of a visual loop-mediated isothermal amplification combined with lateral flow dipstick (LAMP-LFD) method for rapid detection of Salmonella strains in food samples

Cited by 66 publications

References 78 publications

Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow Immunoassay for Rapid Detection of Salmonella in Food

Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow Immunoassay for Rapid Detection of Salmonella in Food

Point-of-Need Diagnostics for Foodborne Pathogen Screening

Rapid and simultaneous detection of Campylobacter spp. and Salmonella spp. in chicken samples by duplex loop-mediated isothermal amplification coupled with a lateral flow biosensor assay

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