Development and application of a novel electrochemical immunosensor for tetracycline screening in honey using a fully integrated electrochemical Bio-MEMS

Abstract

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Development of Novel Magneto-Biosensor for Sulfapyridine Detection

“…Biosensors 2020, 10, x FOR PEER REVIEW 11 of 13 transfer, and finally, W corresponded to Warburg impedance, which is related to the diffusion phenomenon at the electrode-electrolyte interface [10,28]. After calculating fitting parameters from the applied equivalent circuit, it was clearly evidenced that there was more linearity for the biosensor with Ab155 modified MNP ( Figure 11B) when compared to competitive detection without magnetic nanoparticles ( Figure 11A).…”

“…Here, Rs indicated electrolyte solution resistance, Q was the constant phase element that corresponded to the charge distribution at the interface electrode/electrolyte, Rct (which was in parallel with Q) was the resistance of charge transfer, and EIS measurements were repeated at least four times, as we had four Wes, and Nyquist plots were fitted for both Figures 9 and 10 concerning the detection of Ab155 with and without MNP by using the equivalent circuit ( Figure 11A inset). Here, Rs indicated electrolyte solution resistance, Q was the constant phase element that corresponded to the charge distribution at the interface electrode/electrolyte, R ct (which was in parallel with Q) was the resistance of charge transfer, and finally, W corresponded to Warburg impedance, which is related to the diffusion phenomenon at the electrode-electrolyte interface [10,28].…”

“…The biosensor is a compact analytical device or unit incorporating a biological (or biologically) derived sensitive element associated with a physicochemical transducer. They have revolutionized modern analysis due to their technical simplicity, low cost, and the possibility of being employed in field analysis [9,10]. The detection of sulfonamides using biosensors was previously demonstrated in different works [11][12][13].…”

Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs

“…ELISA involving antibodies combined with enzymatic markers has taken a leading position in immunoassay methods [25]. Due to the low molecular weight of the target analytes, competitive ELISA is commonly used for pesticide and veterinary drug residue determination [26,27]. A monoclonal antibody for five antibacterial synergists, 5C4, with uniform broad specificity and high affinity was obtained and used to develop an indirect competitive ELISA (icELISA) method [28].…”

Electrochemical Immunosensors for Antibiotic Detection

“…A new approach, based on a fully integrated bio-microelectromechanical system (Bio-MEMS) containing eight gold microelectrodes (µWEs), was proposed for the impedimetric determination of tetracycline in honey samples [79]. The determination was based on the competition of TC captured on µWes towards a polyclonal TC antibody, utilizing a mixture of a fixed concentration of an antibody and TC solutions of various concentrations.…”