Development and application of a dispersive liquid–liquid microextraction method for the determination of tetracyclines in beef by liquid chromatography mass spectrometry

Mookantsa, Sandy Oshi Squizer; Dube, Simiso; Nindi, Mathew Muzi

doi:10.1016/j.talanta.2015.11.006

Cited by 71 publications

(26 citation statements)

References 17 publications

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“…The proposed analytical method presented good performance when compared with other methods recently published as shown in S9. The obtained LOQs and LODs were equal or even lower.…”

Section: Resultsmentioning

confidence: 73%

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Graphene particles supported on silica as sorbent for residue analysis of tetracyclines in milk employing microextraction by packed sorbent

et al. 2018

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This paper describes the use of graphene-based sorbents for determination of four tetracyclines in milk. The synthesized materials were combined with microextraction by packed sorbent (MEPS) to act as the sample preparation step. The extraction performance of these sorbents was compared to commercial phases, and graphene supported on silica provided the best results. The analytical method optimization was carried out by employing experimental design. Firstly, an evaluation of the experimental variables (elution solvent, use of EDTA, ionic force, and pH of the washing solution) was made by a 2 factorial experimental design. The variables sampling, washing and elution cycles of MEPS were further optimized under a full 2 experimental design. The validation parameters were determined under optimized conditions resulting in a linearity ranging from 15 to 110 μg/L with R values above 0.98, and LOQs ranging from 0.05 to 0.9 μg/L. The accuracy ranged from 87.9 to 118.4% and intra/inter-day precision reported by the RSDs were lower than 19%. The proposed and validated method was successfully applied to the analysis of 11 milk samples from different animals, revealing traces of tetracyclines in only two of them. This study focused on the evaluation of graphene-based sorbents combined with MEPS for tetracycline analysis provided equivalent or even better results than other proposed methods, suggesting being a sensitive, fast and reliable alternative method for the determination of tetracyclines in milk samples.

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“…The proposed analytical method presented good performance when compared with other methods recently published as shown in S9. The obtained LOQs and LODs were equal or even lower.…”

Section: Resultsmentioning

confidence: 73%

“…Analytical methods based on HPLC‐MS are currently the most used to determine TCs due to their high efficiency and selectivity. In the last five years, several results have been reported using this approach to determine TC's and other veterinary drugs in complex food matrices .…”

Section: Introductionmentioning

confidence: 99%

Graphene particles supported on silica as sorbent for residue analysis of tetracyclines in milk employing microextraction by packed sorbent

et al. 2018

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“…The use of these techniques is extensive in water samples, but unfortunately, the application of DLLME (or its variants) on biological samples is more complicated [20]. In complex samples, it is more difficult to obtain a separated floating organic drop due to the interaction of the matrix components with the organic solvents [21].…”

Section: Introductionmentioning

confidence: 99%

Liquid phase microextraction techniques combined with chromatography analysis: a review

Dugheri

Mucci

Bonari

et al. 2020

Acta Chromatographica

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Sample pretreatment is the first and the most important step of an analytical procedure. In routine analysis, liquid–liquid microextraction (LLE) is the most widely used sample pre-treatment technique, whose goal is to isolate the target analytes, provide enrichment, with cleanup to lower the chemical noise, and enhance the signal. The use of extensive volumes of hazardous organic solvents and production of large amounts of waste make LLE procedures unsuitable for modern, highly automated laboratories, expensive, and environmentally unfriendly. In the past two decades, liquid-phase microextraction (LPME) was introduced to overcome these drawbacks. Thanks to the need of only a few microliters of extraction solvent, LPME techniques have been widely adopted by the scientific community. The aim of this review is to report on the state-of-the-art LPME techniques used in gas and liquid chromatography. Attention was paid to the classification of the LPME operating modes, to the historical contextualization of LPME applications, and to the advantages of microextraction in methods respecting the value of green analytical chemistry. Technical aspects such as description of methodology selected in method development for routine use, specific variants of LPME developed for complex matrices, derivatization, and enrichment techniques are also discussed.

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“…To ensure human food safety, a few detection methods have been successfully proposed for determination of TCs, such as gel chromatography , LC , gas–liquid chromatography , and LC–MS/MS . For determination of TCs in milk powder samples, the first step is to purify the low concentrations of analytes in the samples, which requires long sample preparation time.…”

Section: Introductionmentioning

confidence: 99%

Analysis of tetracyclines from milk powder by molecularly imprinted solid‐phase dispersion based on a metal–organic framework followed by ultra high performance liquid chromatography with tandem mass spectrometry

Wang

Zhang

et al. 2018

J of Separation Science

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A novel molecularly imprinted polymer that could selectively recognize tetracyclines in milk powder was synthesized using a metal-organic framework as a support material, tetracycline as template molecule, and 3-aminophenylboronic acid as a functional monomer and a cross-linking agent. The novel molecularly imprinted polymer was characterized by Fourier transform infrared spectrometry, transmission electron microscopy, X-ray diffractometry, thermogravimetric analysis, and N adsorption/desorption measurements. The adsorption isotherms, adsorption kinetics, adsorption thermodynamics, and selective adsorption experiments of the novel molecularly imprinted polymer to tetracycline were also studied. The novel molecularly imprinted polymer was used as dispersant of matrix solid-phase dispersion to extraction tetracyclines. After that, the tetracyclines extracted from milk powder were determined by ultra high performance liquid chromatography with tandem mass spectrometry. Under the optimal conditions, the detection limits of tetracyclines were 0.217-0.318 ng/g. The relative standard deviations of intra- and interday precision ranged from 3.8 to 6.9% and from 2.8 to 7.4%, respectively. In all three concentration levels (1.0, 10, 50 ng/g), the recoveries of tetracyclines ranged from 84.7 to 93.9%. The method was successfully applied to the determination of tetracyclines in milk powder.

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Development and application of a dispersive liquid–liquid microextraction method for the determination of tetracyclines in beef by liquid chromatography mass spectrometry

Cited by 71 publications

References 17 publications

Graphene particles supported on silica as sorbent for residue analysis of tetracyclines in milk employing microextraction by packed sorbent

Graphene particles supported on silica as sorbent for residue analysis of tetracyclines in milk employing microextraction by packed sorbent

Liquid phase microextraction techniques combined with chromatography analysis: a review

Analysis of tetracyclines from milk powder by molecularly imprinted solid‐phase dispersion based on a metal–organic framework followed by ultra high performance liquid chromatography with tandem mass spectrometry

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