Development and application of a method for analysis of phthalates in ham sausages by solid-phase extraction and gas chromatography–mass spectrometry

Guo, Zhiyong; Wang, Sui; Wei, Danyi; Wang, Meili; Zhang, Huina; Gai, Panpan; Duan, Jing

doi:10.1016/j.meatsci.2009.10.002

Cited by 56 publications

(46 citation statements)

References 18 publications

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“…The mathematical relationship between the signal (response) and the concentration of the analysis of interest was expressed by the equation of the line (analytical curve) and its determination coefficient (R 2 ) (Meister 2008). Standard concentrations were back-calculated using the standard regression curve, and the per cent deviation was calculated (Guo et al 2010). Each sample was analysed with triplicate injections.…”

Section: Linearitymentioning

confidence: 99%

“…Selectivity was assessed by checking the retention time of each mycotoxin in the sample to determine if it corresponded to the retention time in the matrix standards and existed for any interfering peaks (Guo et al 2010).…”

Section: Selectivitymentioning

confidence: 99%

“…The results were expressed as a percentage of the quantity of the substance measured relative to the amount added to the matrix, i.e. (concentration found/concentration added) × 100% (Guo et al 2010). Accuracy was tested on the healthy apple fruits spiked with the two analytes at high, intermediate and low concentration levels (1, 0.1 and 0.01 μg g -1 for both T-2 toxin and neosolaniol), and then the concentrations of T-2 toxin and neosolaniol were analysed.…”

Section: Accuracymentioning

confidence: 99%

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A method of analysis for T-2 toxin and neosolaniol by UPLC-MS/MS in apple fruit inoculated withTrichothecium roseum

Tang¹,

Xue²,

Bi³

et al. 2014

Food Additives & Contaminants: Part A

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Trichothecenes are one of the most important groups of mycotoxins produced by Trichothecium roseum, which causes core rot of apple. A reliable and sensitive method was developed and successfully applied for the rapid detection of trichothecenes including T-2 toxin and neosolaniol in harvested apple using UPLC-MS/MS. After the extraction of the two mycotoxins from the apple matrix with methanol/water (80/20, v/v), the concentrated extracts were cleaned-up by PriboFast M270 columns and then analysed by UPLC-MS/MS. T-2 toxin and neosolaniol were effectively separated as unique peaks. The validity of this method was established by its linearity (R(2) ≥ 0.9995), precision (relative standard deviation ≤ 3.6%), accuracy, selectivity, limit of detection of 2-5 μg kg(-1), limit of quantification of 5-10 μg kg(-1) and average recovery of 73-96%. Levels of T-2 toxin were found in the range 7.1-128.4 µg kg(-1) in the core rot lesion of three cultivars apple (cvs. Red Delicious, Fuji and Ralls). T-2 was detected not only in the lesion, but also in the tissue without any disease symptoms. However, neosolaniol was only detected in the lesion on 'Red Delicious' apples. In addition, the concentration of T-2 toxin in the susceptible cultivar (cv. Fuji) was significantly higher than that in the resistant one (cv. Ralls). This method proved to be suitable at detecting T-2 and neosolaniol simultaneously in apples infected with T. roseum.

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Section: Linearitymentioning

confidence: 99%

Section: Selectivitymentioning

confidence: 99%

Section: Accuracymentioning

confidence: 99%

See 1 more Smart Citation

A method of analysis for T-2 toxin and neosolaniol by UPLC-MS/MS in apple fruit inoculated withTrichothecium roseum

Tang¹,

Xue²,

Bi³

et al. 2014

Food Additives & Contaminants: Part A

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show abstract

“…released during use and migrate from the packaging to contaminate the contents such as foods and beverages (Feng et al, 2005;Holadova et al, 2007;Prokupkova et al, 2002;Guo et al, 2010). Some of these compounds are carcinogen and estrogenic (Holadova et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Analysis of phthalate esters contamination in drinking water samples

Kanchanamayoon¹,

Prapatpong²,

S³

et al. 2012

Afr. J. Biotechnol.

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Phthalate esters are widely used as plasticizer. They can migrate from plastic materials to the environment. Exposure to some of these compounds may increase the risk of getting cancer. Phthalate esters: dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP) and bis(2-ethylhexyl) phthalate (DEHP) were extracted simultaneously using a solid-phase extraction and compared with liquid-liquid extraction and analyzed by a gas chromatograph. The optimum condition method was successfully applied to the analysis of phthalate esters contamination in bottled drinking water samples. The concentration of DMP, DEP and DBP in drinking water samples were below allowable levels, while the DEHP concentration in three samples was found to be greater than the regulatory, maximum admissible concentration.

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“…Phthalate acid esters (PAE) or phthalates are widely used in consumable, household, and personal care products contributing to 6.0 million tons annual production worldwide . Phthalates are mainly used as plasticizers to increase flexibility, transparency, durability, and longevity of plastics . The low‐molecular‐weight phthalates, such as diethyl phthalate (DEP) and dimethyl phthalate, are the major constituent of daily life products such as cosmetics, insecticides, paints, and pharmaceutical applications and dibutyl phthalate (DBP) used as solvents to hold color and scent in various consumable and personal care products .…”

Section: Introductionmentioning

confidence: 99%

Simple and rapid determination of phthalates using microextraction by packed sorbent and gas chromatography with mass spectrometry quantification in cold drink and cosmetic samples

Kaur

Heena

Kaur

et al. 2016

J of Separation Science

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A simple and rapid method using microextraction by packed sorbent coupled with gas chromatography and mass spectrometry has been developed for the analysis of five phthalates, namely, diethyl phthalate, benzyl-n-butyl phthalate, dicyclohexyl phthalate, di-n-butyl phthalate, and di-n-propyl phthalate, in cold drink and cosmetic samples. The various parameters that influence the microextraction by packed sorbent performance such as extraction cycle (extract-discard), type and amount of solvent, washing solvent, and pH have been studied. The optimal conditions of microextraction using C18 as the packed sorbent were 15 extraction cycles with water as washing solvent and 3 × 10 μL of ethyl acetate as the eluting solvent. Chromatographic separation was also optimized for injection temperature, flow rate, ion source, interface temperature, column temperature gradient and mass spectrometry was evaluated using the scan and selected ion monitoring data acquisition mode. Satisfactory results were obtained in terms of linearity with R(2) >0.9992 within the established concentration range. The limit of detection was 0.003-0.015 ng/mL, and the limit of quantification was 0.009-0.049 ng/mL. The recoveries were in the range of 92.35-98.90% for cold drink, 88.23-169.20% for perfume, and 88.90-184.40% for cream. Analysis by microextraction by packed sorbent promises to be a rapid method for the determination of these phthalates in cold drink and cosmetic samples, reducing the amount of sample, solvent, time and cost.

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Development and application of a method for analysis of phthalates in ham sausages by solid-phase extraction and gas chromatography–mass spectrometry

Cited by 56 publications

References 18 publications

A method of analysis for T-2 toxin and neosolaniol by UPLC-MS/MS in apple fruit inoculated withTrichothecium roseum

A method of analysis for T-2 toxin and neosolaniol by UPLC-MS/MS in apple fruit inoculated withTrichothecium roseum

Analysis of phthalate esters contamination in drinking water samples

Simple and rapid determination of phthalates using microextraction by packed sorbent and gas chromatography with mass spectrometry quantification in cold drink and cosmetic samples

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