Developing Protocols of Tricine-SDS-PAGE for Separation of Polypeptides in the Mass Range 1-30 kDa with Minigel Electrophoresis System

Enzyme‐assisted extraction (EAE) of oils/fats involves the disruption of the cell wall of source material using enzymes to facilitate the release of oil. When proteases are used as the enzyme, EAE ends in the extracted oil as well as the protein hydrolysates. Herein, the EAE (using a commercial protease, Alcalase) was exploited to obtain fat and protein hydrolysates from chicken skin. Degree of hydrolysis (DH, the percentage ratio of cleaved peptide bonds), which showed a logarithmic correlation with the reaction time, was found to affect the properties of the products. As the DH increased, the peptide chain length of protein hydrolysates decreased which was confirmed by SDS‐PAGE analysis. With the increase of DH, the emulsifying activity index, foaming capacity, and oil holding capacity of the hydrolysates decreased but the solubility and emulsion stability index increased (p < 0.05). The DPPH free radical scavenging activity of the hydrolysates increased with the DH up to DH = 39.62% but decreased thereafter (p < 0.05). EAE resulted in a rise in fat yield and the fat contained a higher amount of unsaponifiables and lower free fatty acids (FFA) content, as compared to the control treatment (No enzyme, 80°C, 2 h, p < 0.05). DH affected the fat yield and the unsaponifiables content of the fat, positively (p < 0.05). However, it did not affect the fat FFA content and iodine value (p > 0.05). Results obtained here showed DH can be used as an effective measure for controlling the physicochemical and functional properties of chicken skin protein hydrolysates and fat in the EAE process.

show abstract

Enzyme‐assisted extraction of chicken skin protein hydrolysates and fat: Degree of hydrolysis affects the physicochemical and functional properties

Razavizadeh

Farmani

Motamedzadegan

2022

J Americ Oil Chem Soc

View full text Add to dashboard Cite

show abstract

Evaluation of protein content and antimicrobial activity of biomass fromSpirulinacultivated with residues from the brewing process

Pereira

Lisbôa

Costa

et al. 2021

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND: Spirulina is a microalga that has a high protein content, in addition to having nutritional and pharmaceutical properties; therefore, it is a potential source for obtaining bioactive peptides. The obtaining of biocomposites of interest from microalgal biomass can be influenced by factors of cultivation, such as aeration, temperature, culture medium, and pH. Thus, this work aimed at evaluating the protein contents and antimicrobial activity of hydrolyzed and non-hydrolyzed biomass of the Spirulina microalgae grown under optimized conditions of the brewing residue by applying Design of Experiments (DOE) framework. The influence of airflow and concentration of brewing residue on microalgal culture was firstly analyzed using the Design of Experiments technique. The cultivation was carried out on a larger scale with subsequent enzymatic hydrolysis of the microalgal protein extract to analyze the hydrolysis degree, protein content, and antimicrobial activity. RESULTS:The results obtained indicated that there are no significant effects of the factors evaluated on the response variables. However, the obtained biomass showed a high protein content, above 51.88%. Regarding the extraction and hydrolysis of microalgae proteins, a degree of hydrolysis of 52.28% was obtained; however, the analyzed samples did not show antimicrobial activity under the studied conditions. CONCLUSION: Thus, it is demonstrated that the cultivation of Spirulina with residues from the brewing process has the potential for obtaining compounds of interest, such as proteins.

show abstract

Evaluation of genetic diversity using biochemical markers in sugarcane germplasm collection

Singh,

Mishra

et al. 2023

Vegetos

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Developing Protocols of Tricine-SDS-PAGE for Separation of Polypeptides in the Mass Range 1-30 kDa with Minigel Electrophoresis System

Cited by 14 publications

References 8 publications

Enzyme‐assisted extraction of chicken skin protein hydrolysates and fat: Degree of hydrolysis affects the physicochemical and functional properties

Enzyme‐assisted extraction of chicken skin protein hydrolysates and fat: Degree of hydrolysis affects the physicochemical and functional properties

Evaluation of protein content and antimicrobial activity of biomass fromSpirulinacultivated with residues from the brewing process

Evaluation of genetic diversity using biochemical markers in sugarcane germplasm collection

Contact Info

Product

Resources

About