Developing centrifugal spun thermally cross‐linked gelatin based fibrous biomats for antibacterial wound dressing applications

Abstract

Fibrous materials obtained from natural polymers, such as gelatin, have been used in medical applications due to their biocompatibility and biodegradability. Herein, free‐standing durable fibrous gelatin biomats with antibacterial activity were developed via a simple, low cost and fast production route, centrifugal spinning, and subsequent thermal crosslinking. After a series of preliminary experiments, droplet−/bead‐free porous biomats with fine fibers, 3.41 ± 1.8 μm in diameter, were fabricated. Subsequently, antimicrobial biomats were produced by adding AgNO3 into the production solution. X‐ray diffractometer and energy dispersive X‐ray results showed Ag NPs existing as AgCl in the biomats, which could be attributed to chemical reaction between the Ag NPs and residual Cl in the impure gelatin. Later, both the neat‐gelatin and Ag‐gelatin biomats were thermally crosslinked at 170°C to gain stability against water. Although the Ag addition reduced ultimate tensile strength by half, from 881 to 495 kPa, the crosslinked biomats were robust enough to be used for wound dressing applications. They were also found to be highly breathable, with the air permeability of 256 and 81.2 mm/s, respectively. The biomats showed antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria. The results show that the free‐standing fibrous‐gelatin‐based biomats produced is applicable for wound dressing applications.

Effect of OHMIC heating and ultrasound on functional properties of biodegradable gelatin‐based films

“…Figure 4 shows the diffraction peaks for control, OH30, and US100 films. As observed, all films presented a diffraction peak at 2θ = 20.62-21.58 , corresponding to random coiled conformation (amorphous fractions) of the gelatin macromolecules, as similarly reported by Liu et al [66] and Gungor et al [67] In addition, another less intense peak was observed at 2θ = 7.74-9.02 , corresponding to the triple-helical crystalline structures of gelatin. [68,69] The treated films showed higher intensity in the triple helix peak than the control film, which indicates higher levels of triple helix renaturation.…”

State-of-the-Art Review of Electrospun Gelatin-Based Nanofiber Dressings for Wound Healing Applications

“…Wang et al employed pullulan, which could form hydrogen bonds with gelatin, to stabilize the electrospun gelatin nanofibers, leading to obviously enhanced mechanical properties [ 79 ]. Gungor et al used a thermally crosslinked strategy to stabilize the structure and improve the water-resistant property [ 80 ]. In addition, an ultraviolet (UV) irradiation was used to physically crosslink the electrospun gelatin nanofiber mats by Beishenaliev and coworkers [ 81 ].…”

Fabrication and evaluation of homogeneous alginate/polyacrylamide–chitosan–gelatin composite hydrogel scaffolds based on the interpenetrating networks for tissue engineering

“…[23] It is worth noting that the GT molecular chain contains the arginine-glycine-aspartic (RGD) polypeptide sequence that is a specific protein recognized by cells and their ECM, which can regulate cell adhesion and physiology activity. [24,25] Therefore, the LBL electrostatic assembly performed by alternate deposition of polyelectrolytes via electrostatic interaction on the surface of Alg/PAAM hydrogels may provide them with new functionalities. [26] In the present study, to develop the ideal scaffold for tissue engineering, the interpenetrating network technology and LBL electrostatic assembly technology were proposed to prepare the homogeneous alginate/polyacrylamidechitosan-gelatin (Alg/PAAM-CS-GT) composite hydrogel scaffolds.…”

“…[ 23 ] It is worth noting that the GT molecular chain contains the arginine–glycine–aspartic (RGD) polypeptide sequence that is a specific protein recognized by cells and their ECM, which can regulate cell adhesion and physiology activity. [ 24, 25 ] Therefore, the LBL electrostatic assembly performed by alternate deposition of polyelectrolytes via electrostatic interaction on the surface of Alg/PAAM hydrogels may provide them with new functionalities. [ 26 ]…”