Developing and Optimizing an Immunoaffinity Cleanup Technique for Determination of Quinolones from Chicken Muscle

Abstract

An immunoaffinity chromatographic method was developed using an antibody mediated immunosorbent to selectively extract and purify 10 quinolones (marbofloxacin, norfloxacin, ciprofloxacin, lomefloxacin, danofloxacin, enrofloxacin, difloxacin, sarafloxacin, oxolinic acid, and flumequine) in chicken muscle followed by HPLC. The operating conditions of the immunoaffinity chromatography (IAC) column were optimized, and the IAC has been successfully used for the isolation and purification of 10 quinolones from chicken muscle tissue. The optimized immunoaffinity column sample cleanup procedure combined with HPLC coupling to fluorescence detection afforded low limits of detection (0.1 ng g(-1) for danfloxacin and 0.15 ng g(-1) for all other quinolones tested). The method was also applied to determine quinolone residues in commercial muscle samples.

Development and characterization of a chitosan-supported immunoaffinity chromatography column for the selective extraction of methandrostenolone from food and feed samples

“…In the field of food safety, IAC has been widely used in sample pretreatment for residue analysis of mycotoxin, veterinary drugs, pesticides, phycotoxins, process and environmental contaminants and vitamins [5][6][7][8]. Sepharose is commonly used as support matrix for chromatography because of its porosity, chemical and mechanical stability.…”

Immunoaffinity column clean-up techniques in food analysis: A review

“…Although a range of monoclonal antibodies are commercially available from a number of suppliers, preparing columns does limit applicability to those with the skills and experience to couple the antibody to a gel and be able to prepare a robust column. Nevertheless, many recent publications still involve the use of IACs prepared in the authors own laboratory [32][33][34], and although full experimental details of preparation are given, most analytical chemists will err towards purchasing commercial columns if available. A comparison of commercial and in-house columns [35] clearly showed that even some commercial columns can fail to adequately entrap and then to elute the analyte.…”

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

“…Using an immunoaffinity column the group successfully developed a method to isolate 13 quinolones and six SAs in swine and chicken muscle. Zhao and Li et al used the same method to isolate 10 quinolones in chicken muscle but used HPLC-FL for analysis [123]. The authors note the possibility of greater selectivity when using such IA columns, compared with the smaller range of interaction mechanisms available for exploitation with more conventional SPE formats.…”