Developing and Optimizing a Chronic Cyanotic Swine Model

Gerrah, Rabin; Cabreriza, Santos E.; Rusanov, Alexander; Romanov, Alexander; Khan, Azeem; Spotnitz, Henry M.

doi:10.1016/j.jss.2010.03.004

Cited by 3 publications

(2 citation statements)

References 15 publications

(14 reference statements)

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“…The idea behind this study was based on our previous research experience, when we observed fewer thrombotic events in a PTFE graft when it was exposed to heparin before implantation. 13…”

Section: Discussionmentioning

confidence: 99%

Pretreatment of synthetic vascular grafts with heparin before implantation, a simple technique to reduce the risk of thrombosis

2014

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Thrombosis of synthetic grafts commonly used in cardiovascular surgery is a major complication. We examined whether pretreatment of the graft with heparin reduces the risk of early thrombosis. A circuit was assembled to compare two pairs of shunts simultaneously in the same animal. The study shunts were pretreated with heparin. After 2 hours of circulation, clot formation was evaluated by image analysis techniques. The pretreated grafts had fewer blood clots adhered to the surface by direct visual inspection. The image analysis showed 5 vs. 39 clots, 0.01% vs. 1.8% clotted area, and 62 vs. 5630 clot pixel area between the treated and non-treated grafts respectively, p < 0.05. Pretreatment of the synthetic graft with heparin prior to implantation reduces the risk of early clot formation. This simple practice might be helpful to prevent initial thrombosis of the graft and later occlusion.

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“…The idea behind this study was based on our previous research experience, when we observed fewer thrombotic events in a PTFE graft when it was exposed to heparin before implantation. 13…”

Section: Discussionmentioning

confidence: 99%

Pretreatment of synthetic vascular grafts with heparin before implantation, a simple technique to reduce the risk of thrombosis

2014

Self Cite

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“…To examine this issue of myocardial protection in the setting of sudden hyperoxia superimposed on cyanosis, we enhanced a previously described model of chronic cyanosis [68]. A left atrial-pulmonary artery shunt was constructed in piglets, and the distal pulmonary artery was encircled with a Teflon band to produce relative pulmonary stenosis during growth.…”

Section: Piglet Model Of Surgery For Cyanotic Congenital Heart Diseasementioning

confidence: 99%

Ventricular Function in Surgery for Congenital Heart Disease

Spotnitz

2009

World j. surg.

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BACKGROUND The history of measuring myocardial edema by two-dimensional echocardiography and the pathophysiology of myocardial edema are reviewed. METHODS The relevance of this subject to management of children undergoing corrective surgery for single ventricle physiology and tetralogy of Fallot is reviewed. RESULTS Evidence is presented that myocardial edema is an ongoing clinical problem with relevance to management and outcomes. Methods for measuring mass increases noninvasively in the range of 10-25% with increases in myocardial water content on the order of 2-4% are now well established. CONCLUSIONS These methods and advanced animal models replicating conditions of surgery for cyanotic congenital heart disease set the stage for clinical advances in this important area.

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Vascular Histopathologic Reaction to Pulmonary Artery Banding in an In Vivo Growing Porcine Model

et al. 2013

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Pulmonary artery banding (PAB) is used as a surgical palliation to reduce excessive pulmonary blood flow caused by congenital heart defects. Due to the lack of microscopic studies dealing with the tissue remodeling caused by contemporary PAB materials, this study aimed to assess histologic changes associated with PAB surgery by analyzing local tissue reaction to the presence of Gore-Tex strips fixed around the pulmonary artery. Gore-Tex strips were used for PAB in a growing porcine model. After 5 weeks, histologic samples with PAB (n = 5) were compared with healthy pulmonary arterial segments distal to the PAB or from a sham-treated animal (n = 1). Stereology was used to quantify the density of the vasa vasorum and the area fraction of elastin, smooth muscle actin, macrophages, and nervi vasorum within the pulmonary arterial wall. The null hypothesis stated that samples did not differ histopathologically from adjacent vascular segments or sham-treated samples. The PAB samples had a greater area fraction of macrophages, a lower amount of nervi vasorum, and a tendency toward decreased smooth muscle content compared with samples that had no PAB strips. There was no destruction of elastic membranes, no medionecrosis, no pronounced inflammatory infiltration or foreign body reaction, and no vasa vasorum deficiency after the PAB. All the histopathologic changes were limited to the banded vascular segment and did not affect distal parts of the pulmonary artery. The study results show the tissue reaction of palliative PAB and suggest that Gore-Tex strips used contemporarily for PAB do not cause severe local histologic damage to the banded segment of the pulmonary arterial wall after 5 weeks in a porcine PAB model.

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Developing and Optimizing a Chronic Cyanotic Swine Model

Cited by 3 publications

References 15 publications

Pretreatment of synthetic vascular grafts with heparin before implantation, a simple technique to reduce the risk of thrombosis

Pretreatment of synthetic vascular grafts with heparin before implantation, a simple technique to reduce the risk of thrombosis

Ventricular Function in Surgery for Congenital Heart Disease

Vascular Histopathologic Reaction to Pulmonary Artery Banding in an In Vivo Growing Porcine Model

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