The Department of Biology, Universitas Negeri Malang, manages undergraduate courses to prepare prospective teachers of Biology. Biology teachers have to know two important purposes of science education, that is, educating and motivating students to be involved in careers such as scientists and technologists, and providing students with the significant knowledge and understanding of science and social issues to enable them to become informed citizens. This research is conducted to investigate scientific literacy of the third year students of the department. Scientific literacy skill is measured by Multidimensional Literacy Test (MLT), a combination of multiple choice and essay test. The results showed that Scientific Literacy of the third year students is on the level of functional and structural dimension. The result of the interviews with the teachers about the teaching strategies showed that learning scientific writing, which is an important basis scientific literacy development, still needs to be improved.The results of the study can be used as a consideration in selecting and determining appropriate learning strategies to improve the literacy of prospective biology teachers.