Developing a preventive immunization approach against insect bite hypersensitivity using recombinant allergens: A pilot study

Jónsdóttir, Sigríður; Hamza, Eman; Janda, Jozef; Rhyner, Claudio; Meinke, Andreas; Marti, Eliane; Svansson, Vilhjálmur; Torsteinsdóttir, Sigurbjörg

doi:10.1016/j.vetimm.2015.05.002

Cited by 31 publications

(37 citation statements)

References 66 publications

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“…The protein was purified under denaturing conditions with the HIS‐select™ nickel affinity gel (Sigma, St. Louis, MO, USA, https://www.sigmaaldrich.com) according to protocol and dialysed against 4 M urea. After dialysis, Bac‐rCul o 3 was analysed with SDS‐PAGE and the gel stained with Coomassie blue, (PhastGel Blue R, Sigma) using standard protocols (Figure ) or transferred to a PVDF membrane (Millipore, Hellerup, Denmark, http://www.merckmillipore.com) and detected with mouse anti‐His antibody (Bio‐Rad, Hercules, CA, USA, https://www.bio-rad-antibodies.com), diluted 1:1,000, followed by the standard procedure of Western blot, as described previously …”

Section: Methodsmentioning

confidence: 99%

“…Allergen‐specific serum IgE levels against the ten recombinant C. nubeculosus allergens and NubTE, as well as against the three C. obsoletus r‐allergens and ObsWBE were determined by indirect ELISA. Levels of serum IgG subclasses against three of the most relevant rCulicoides allergen, rCul n 4, rCul o 1, rCul o 3, and NubTE and equine Influenza, were determined by ELISA, as described previously . Briefly, microtitre plates were coated with 2 μg/mL allergens, followed by blocking with blocking buffer (Phosphate buffered saline containing 5% dry milk powder and 5% Tween‐20).…”

Section: Methodsmentioning

confidence: 99%

“…After dialysis, Bac-rCul o 3 was analysed with SDS-PAGE and the gel stained with Coomassie blue, (PhastGel Blue R, Sigma) using standard protocols ( Figure S1) or transferred to a PVDF membrane (Millipore, Hellerup, Denmark, http://www.merck millipore.com) and detected with mouse anti-His antibody (Bio-Rad, Hercules, CA, USA, https://www.bio-rad-antibodies.com), diluted 1:1,000, followed by the standard procedure of Western blot, as described previously. 15 Thorax extract from C. nubeculosus (NubTE) and whole body extract from C. obsoletus complex midges (ObsWBE) were prepared as described previously. 5 Equine Influenza A Fontainebleau 79 inactivated virus (APHA, Weybridge, Surrey, UK; EURL Influenzavirus), was used as a control antigen in the IgG-subclasses enzyme-linked immunosorbent assay (ELISA), because Icelandic horses are not vaccinated against equine Influenza in Iceland, whereas they are vaccinated following import into Switzerland.…”

Section: Allergens and Antigensmentioning

confidence: 99%

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Longitudinal analysis of allergen‐specific IgE and IgG subclasses as potential predictors of insect bite hypersensitivity following first exposure to Culicoides in Icelandic horses

Ziegler

Hamza

Jónsdóttir

et al. 2017

Veterinary Dermatology

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Background -Insect bite hypersensitivity (IBH) is an allergic dermatitis of horses caused by bites of Culicoides spp. IBH does not occur in Iceland because of the absence of Culicoides, but the prevalence is high in horses imported from Iceland to environments where Culicoides are present.Hypothesis/Objective -Test, in a longitudinal study before and after Culicoides exposure, whether a primary sensitizing Culicoides allergen can be identified and if an increase of allergen-specific immunoglobulin (Ig)E or IgG subclasses precedes clinical signs of IBH.Animals -Thirty two horses imported from Iceland to Europe; 16 developed IBH and 16 remained healthy. Methods -Determination of IgE and IgG subclasses against recombinant (r)-Culicoides allergens andCulicoides extract in sera taken before first exposure to Culicoides and yearly over a period of 3-4 years.Results -Before Culicoides exposure, there were no significant differences in Culicoides-specific serum IgE levels between horse that developed IBH or remained healthy. Culicoides exposure induced an individual IgE response pattern (to a median of 4.5 r-allergens) in the IBH but not in the healthy end-point group. The increase in serum IgE levels to Culicoides r-allergens was concurrent with the initial onset of clinical signs of IBH. IBHaffected horses displayed significantly higher allergen-specific IgG1 and IgG5 levels than healthy controls. Recombinant Culicoides obsoletus 1 (Cul o1) and Cul o3-specific IgG5 was significantly higher in the IBH compared to the healthy end-point group, before clinical signs of IBH.Conclusion/Clinical relevance -Allergen-specific serum IgE cannot be used as predictor for IBH, whereas allergen-specific IgG5 levels may have a predictive value.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Allergens and Antigensmentioning

confidence: 99%

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Longitudinal analysis of allergen‐specific IgE and IgG subclasses as potential predictors of insect bite hypersensitivity following first exposure to Culicoides in Icelandic horses

Ziegler

Hamza

Jónsdóttir

et al. 2017

Veterinary Dermatology

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“…IgG1 and IgG4/7 are thought to be essential for protection against viral and bacterial infections while the IgG3 and IgG5 have been associated with Th2 response and extracellular pathogens [22]. A high IgG4/7 background to E. coli expressed proteins has been observed previously in sera from some horses [14] and may be due to IgG4/7 antibodies against trace amounts of contaminating E. coli proteins present in the recombinant protein (purity of 95% [4]). This was also the case for IgG4/7, probably due to the fact that the pre-treatment sera showed very high IgG4/7 background on the E. coli-rCul n 2, used in the ELISA.…”

Section: Discussionmentioning

confidence: 92%

Oral administration of transgenic barley expressing a Culicoides allergen induces specific antibody response

Jónsdóttir

Svansson

Stefánsdóttir

et al. 2016

Equine Veterinary Journal

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“…It is a reaction towards allergens present in the saliva of these biting midges. IBH has been described as a type I hypersensitivity reaction with release of histamine and other inflammatory mediators from basophils and mast cells (Jonsdottir et al, 2015). However the involvement of a type IV hypersensitivity reaction has also been suggested (Jonsdottir et al, 2015;Schaffartzik et al, 2012).…”

Section: Insect Bite Hypersensitivity Backgroundmentioning

confidence: 99%

Genetics of equine insect bite hypersensitivity and genetic diversity in horses

Shrestha¹

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Genetic variation contributing to the phenotypic variation was utilized in this thesis to understand the genetic background of a complex trait IBH, and to understand genetic diversity and relationships between various horse populations.IBH is the most common skin allergic disorder in horses, caused by bites of midges, predominantly Culicoides species. It affects various horse breeds worldwide. With no effective treatment, IBH degrades horse health and causes economic loss. In this thesis, we used genome-wide SNPs to identify regions contributing to genetic variance of IBH susceptibility. We also investigated influence of increased number of horses and dense SNPs on identification of genomic regions associated to IBH susceptibility. Multiple genomic regions with small effects were observed in Studies I-III. Interesting genomic regions in the Icelandic horse population across the studies I and II, was observed on chromosomes 1, 7, 10, 15 and 17. The percentage of the genetic variance explained by top ten windows increased from 3.07% (Study I) to 6.56% (Study II). Novel genomic regions were identified when number of Icelandic horses was increased in Study II. Using dense SNPs on the Exmoor pony population we identified novel genomic regions, on chr 8, associated to IBH susceptibility, though with borderline significance.In Study IV, pre-conceived understanding about evolutionary history of horse populations matched obtained results from investigation of genetic relationships within Dutch warmblood populations (pairwise mean FST ≤ 0.070), and within pony-like populations (pairwise mean FST ≤ 0.078). Horse populations with similar genetic background might share similar genetic components for IBH susceptibility. The Friesian horse population had lowest diversity (mean inbreeding coefficients: fi: 30.4%, fiROH= 22.2%) in Study IV and was genetically distinct (FST ranged from 0.13 to 0.17). This might be a result of a history of several population bottlenecks and selection on a closed breeding scheme. Low diversity in immunity related genes, observed in the Friesian horse population, might have led to increased prevalence of IBH. Similarly, low susceptibility of IBH in a warmblood population, KWPN sport horse population might be due to high genetic diversity ( i f =-6.9%). High genetic diversity in KWPN sport horse population might be a result of an open breeding scheme and interbreeding with other warmblood populations.

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Developing a preventive immunization approach against insect bite hypersensitivity using recombinant allergens: A pilot study

Cited by 31 publications

References 66 publications

Longitudinal analysis of allergen‐specific IgE and IgG subclasses as potential predictors of insect bite hypersensitivity following first exposure to Culicoides in Icelandic horses

Longitudinal analysis of allergen‐specific IgE and IgG subclasses as potential predictors of insect bite hypersensitivity following first exposure to Culicoides in Icelandic horses

Oral administration of transgenic barley expressing a Culicoides allergen induces specific antibody response

Genetics of equine insect bite hypersensitivity and genetic diversity in horses

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