Developing a novel immunochromatographic test strip with gold magnetic bifunctional nanobeads (GMBN) for efficient detection of Salmonella choleraesuis in milk

Xia, Shiqi; Yu, Zhongjian; Liu, Daofeng; Xu, Chaolian; Lai, Weihua

doi:10.1016/j.foodcont.2015.06.028

Cited by 82 publications

(25 citation statements)

References 23 publications

(21 reference statements)

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“…These particles separate the target bacteria from the samples by using magnetism and enrich these bacteria in the magnetic field. When GMBN based LFA was compared with traditional LFA, it was more sensitive and had shorter detection time [216].…”

Section: Tablementioning

confidence: 99%

Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

484

315

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Section: Tablementioning

confidence: 99%

Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

484

315

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“…The limits of detection were 12 CFU/mL in broth and 30-300 CFU/mL in food matrices. Xia et al [64] developed a gold magnetic bifunctional nanobead-based lateral flow assay for the detection of Salmonella choleraesuis. Results indicated that the assay was specific and rapid with the detection limit of 5 Â 10 5 CFU/mL, which was much more sensitive than that of colloid gold-based LFIA (5 Â 10 6 CFU/mL), suggesting that magnetic beads were indeed superior to colloid gold.…”

Section: Magnetic Beads As Labelmentioning

confidence: 99%

Current and Emerging Technologies for Rapid Detection of Pathogens

Zeng¹,

Wang²,

Hu³

2018

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

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Foodborne diseases, caused by pathogenic bacteria, have become an important social issue in the field of food safety. It presents a widespread and growing threat to human health in both developed and developing countries. As such, techniques for the detection of foodborne pathogens and waterborne pathogens are urgently needed to prevent the occurrence of human foodborne infections. Although traditional culture-based bacterial isolation and identification are the "gold standard" methods with high preciseness, their drawbacks in time-consuming are inadequate for rapid detection of pathogen to reduce foodborne disease occurrence. Fortunately, with the development of biotechnologies and nanotechnologies, various kinds of new technologies for rapid detection of pathogens have been developed so far, such as nucleic acid-based methods, antibody-based methods, and aptamer-based assays. In this chapter, we summarized the principles and the application of some recent rapid detection technologies for pathogenic bacteria. Moreover, the advantages and disadvantages of the established and emerging rapid detection methods are addressed here.

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“…The a-La's concentrations ranged from 0.1-1000 ng mL À1 , the half maximal inhibitory concentration (IC 50 ) was 0.03 mg mL À1 , and LOD was 0.1 ng mL À1 , showing that the established FLISA had sensitivity and was successfully applied to the detection of a-La in commercial dairy products. 15 There are also other methods using QDs such as impedance, 16 ELISA, 17 test strips, 18,19 and PCR for detecting bacteria. 20,21 Above all, FLISA has been widely used for detection in many elds, while the application of FLISA in microbiological detection is correspondingly rare.…”

Section: Introductionmentioning

confidence: 99%