A fluorescence immunoassay based on CdTe : Zn/ZnS quantum dots for the rapid detection of bacteria, taking <i>Delftia tsuruhatensis</i> CM’13 as an example

Ruan, Yao; Xu, Hang; Yu, Jinlu; Chen, Qian; Gu, Lihong

doi:10.1039/c9ra08651j

Cited by 4 publications

(2 citation statements)

References 23 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Normally, enzyme-labeled secondary antibodies are need for ELISA to determine the absorbance of the substrate [ 4 ]. Distinct from the ELISA, fluorescence-linked immunosorbent assay (FLISA) employs a fluorescent substance as a label to measure the fluorescence intensity [ 5 ]. Both ELISA and FLISA are efficient high-throughput detection methods.…”

Section: Introductionmentioning

confidence: 99%

Polydopamine-Functionalized Copper Peroxide/ZIF-8 Nanoparticle-Based Fluorescence-Linked Immunosorbent Assay for the Sensitive Determination of Carcinoembryonic Antigen by Self-Supplied H2O2 Generation

et al. 2022

View full text Add to dashboard Cite

Copper peroxide/zeolitic imidazolate framework/polydopamine nanoparticles (CP/ZIF-8/PDA)-based fluorescence-linked immunosorbent assay (FLISA) was designed for the sensitive and high-throughput determination of carcinoembryonic antigen (CEA) by self-supplied H2O2 generation. Specifically, the CEA aptamer was modified on the surface of CP/ZIF-8/PDA to form an immunoprobe. The structures of CP and ZIF-8 could be broken under acidic conditions, and produced the Cu2+ and H2O2 due to the dissociation the CP. A subsequent Fenton-type reaction of Cu2+ and H2O2 generated hydroxyl radical (·OH). o-phenylenediamine (OPD) was oxidized by the ·OH to form 2, 3-diaminophenazine (DPA) with a significant fluorescence signal. CP/ZIF-8/PDA could be used as an efficient Fenton-type reactant to generate a large amount of ·OH to promote OPD oxidation. The sensitive detection of CEA could be realized. Under optimal conditions, the FLISA platform displayed a linear detection range from 0.01 to 20 ng mL−1 with a detection limit of 7.6 pg mL−1 for CEA. This strategy has great application potential for sensitive and high-throughput determination for other biomarkers in the field of biomedicine.

show abstract

Section: Introductionmentioning

confidence: 99%

Polydopamine-Functionalized Copper Peroxide/ZIF-8 Nanoparticle-Based Fluorescence-Linked Immunosorbent Assay for the Sensitive Determination of Carcinoembryonic Antigen by Self-Supplied H2O2 Generation

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In addition, CdTe quantum dots (QDs) were selected as the fluorescent emitter model in this study. The excellent properties of QDs, such as controlled emission wavelength, high fluorescence quantum yield, and good biocompatibility, − can improve the performance and application of SPCE-based systems.…”

mentioning

confidence: 99%

Amplified Fluorescence by Hollow-Porous Plasmonic Assembly: A New Observation and Its Application in Multiwavelength Simultaneous Detection

et al. 2021

View full text Add to dashboard Cite

Surface plasmon coupled emission (SPCE) is a new analytical technique that provides increased and directional radiation based on the near-field interaction between fluorophores and surface plasmons but suffers from the limitation of insufficient sensitivity. The assembly of hollow-porous plasmonic nanoparticles could be the qualified candidate. After the introduction of gold nanocages (AuNCs), fluorescence signal enhancement was realized by factors over 150 and 600 compared with the normal SPCE and free space emission, respectively, with a fluorophore layer thickness of approximately 10 nm; hence, the unique enhancement of SPCE by the AuNCs effectively overcomes the signal quenching induced by resonance energy transfer (in normal SPCE). This enhancement was proven to be triggered by the superior wavelength match, the enhanced electromagnetic field, and new radiation channel and process induced by the AuNC assembly, which provides an opportunity to increase the detection sensitivity and establish an optimal plasmonic enhancement system. The amplified SPCE system was employed for multiwavelength simultaneous enhancement detection through the assembly of mixed hollow nanoparticles (AuNCs and gold nanoshells), which could broaden the application of SPCE in simultaneous sensing and imaging for multianalytes.

show abstract

Water soluble silicon quantum dot based fluorescence immunoassay probe for C-reactive protein (CRP) detection

Sebastian

Arif

Kala

2023

Journal of Photochemistry and Photobiology A: Chemistry

View full text Add to dashboard Cite

A fluorescence immunoassay based on CdTe : Zn/ZnS quantum dots for the rapid detection of bacteria, taking Delftia tsuruhatensis CM’13 as an example

Abstract: A fluorescence immunoassay has been widely applied in different fields due to its high sensitivity, simple operations, and high accuracy.

Cited by 4 publications

References 23 publications

Polydopamine-Functionalized Copper Peroxide/ZIF-8 Nanoparticle-Based Fluorescence-Linked Immunosorbent Assay for the Sensitive Determination of Carcinoembryonic Antigen by Self-Supplied H2O2 Generation

Polydopamine-Functionalized Copper Peroxide/ZIF-8 Nanoparticle-Based Fluorescence-Linked Immunosorbent Assay for the Sensitive Determination of Carcinoembryonic Antigen by Self-Supplied H2O2 Generation

Amplified Fluorescence by Hollow-Porous Plasmonic Assembly: A New Observation and Its Application in Multiwavelength Simultaneous Detection

Water soluble silicon quantum dot based fluorescence immunoassay probe for C-reactive protein (CRP) detection

Contact Info

Product

Resources

About