“…Previous investigations conducted on the Ultraflex III MS coupled with MCP-TPX assembly and the modified LCT (nanoESI-orthogonal acceleration reflectron TOF) MS coupled with HV-floating TPX quad system demonstrated the ability of TPX to detect intact protein ions with m / z values up to 400,000 Da and multiply charged non-covalent protein complexes of molecular weight up to 800,000 Da ( m / z < 13,000 Da). , Here, we extend the m / z detection range of TPX family up to 970,000 Da by the measurement of single-charged intact immunoglobulin M (IgM) ions using the MCP-phosphor screen-TPX3CAM assembly, which has previously been used with great success in velocity map imaging to investigate small-molecule reaction dynamics. − In addition, we demonstrate the utilization of multiple information provided by TPX3 such as TOA, pixel coordinates, and TOT for better visualization of the mass spectrum. We discuss how the reduced dead time and event-based readout in TPX3 compared to the TPX enhances both the low and high mass measurements ( m / z mass range: 757–970,000 Da) and allows the operation of the whole system 10 times faster.…”