DEVD-Based Hydrogelator Minimizes Cellular Apoptosis Induction

Tang, Aihua; Wang, Wei‐juan; Mei, Bin; Hu, Wanglai; Wu, Mian; Liang, Gaolin

doi:10.1038/srep01848

Cited by 19 publications

(7 citation statements)

References 38 publications

(39 reference statements)

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“…Inhibition of cancer cells (breast cancer cell line, MCF7) proliferation was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction. 64 MCF-7 cells were seeded at a density of 10 000 cells per well in a 96-well plate for 18−24 h before treatment with the hydrogelator. Then cells were treated with various concentrations of the compound (stock concentration was 10 mM in autoclaved water) in DMEM medium containing 10% fetal bovine serum for 24h in 96-well plate (Figure 9).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Assembly of an Injectable Noncytotoxic Peptide-Based Hydrogelator for Sustained Release of Drugs

et al. 2014

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A new synthetic tripeptide-based hydrogel has been discovered at physiological pH and temperature. This hydrogel has been thoroughly characterized using different techniques including field emission scanning electron microscopic (FE-SEM) and high-resolution transmission electron microscopic (HR-TEM) imaging, small- and wide-angle X-ray diffraction analyses, FT-IR, circular dichroism, and rheometric analyses. Moreover, this gel exhibits thixotropy and injectability. This hydrogel has been used for entrapment and sustained release of an antibiotic vancomycin and vitamin B12 at physiological pH and temperature for about 2 days. Interestingly, MTT assay of these gelator molecules shows almost 100% cell viability of this peptide gelator, indicating its noncytotoxicity.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Assembly of an Injectable Noncytotoxic Peptide-Based Hydrogelator for Sustained Release of Drugs

et al. 2014

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“…Western blot analysis indicated that the isomer 474 , which is not a substrate of caspase-3, at 400 μM, obviously is able to activate caspase-3 to induce cell death via apoptosis. 985 …”

Section: Applicationsmentioning

confidence: 99%

Supramolecular Hydrogelators and Hydrogels: From Soft Matter to Molecular Biomaterials

et al. 2015

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In this review we intend to provide a relatively comprehensive summary of the work of supramolecular hydrogelators after 2004 and to put emphasis particularly on the applications of supramolecular hydrogels/hydrogelators as molecular biomaterials. After a brief introduction of methods for generating supramolecular hydrogels, we discuss supramolecular hydrogelators on the basis of their categories, such as small organic molecules, coordination complexes, peptides, nucleobases, and saccharides. Following molecular design, we focus on various potential applications of supramolecular hydrogels as molecular biomaterials, classified by their applications in cell cultures, tissue engineering, cell behavior, imaging, and unique applications of hydrogelators. Particularly, we discuss the applications of supramolecular hydrogelators after they form supramolecular assemblies but prior to reaching the critical gelation concentration because this subject is less explored but may hold equally great promise for helping address fundamental questions about the mechanisms or the consequences of the self-assembly of molecules, including low molecular weight ones. Finally, we provide a perspective on supramolecular hydrogelators. We hope that this review will serve as an updated introduction and reference for researchers who are interested in exploring supramolecular hydrogelators as molecular biomaterials for addressing the societal needs at various frontiers.

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“…Because it is easy to observe sol–gel phase transition, the use of enzymes to trigger the formation of supramolecular hydrogels becomes a facile assay to identify the substrates for ENS. Some of those examples ,,− have been summarized in a recent review on supramolecular hydrogels . The following section mainly discusses the works published after that review according to the types of the enzymes.…”

Section: Enzymatic Noncovalent Synthesis In Cell-free Conditionsmentioning

confidence: 99%

Enzymatic Noncovalent Synthesis

et al. 2020

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Enzymatic reactions and noncovalent (i.e., supramolecular) interactions are two fundamental nongenetic attributes of life. Enzymatic noncovalent synthesis (ENS) refers to a process where enzymatic reactions control intermolecular noncovalent interactions for spatial organization of higher-order molecular assemblies that exhibit emergent properties and functions. Like enzymatic covalent synthesis (ECS), in which an enzyme catalyzes the formation of covalent bonds to generate individual molecules, ENS is a unifying theme for understanding the functions, morphologies, and locations of molecular ensembles in cellular environments. This review intends to provide a summary of the works of ENS within the last decade and emphasize ENS for functions. After comparing ECS and ENS, we describe a few representative examples where nature uses ENS, as a rule of life, to create the ensembles of biomacromolecules for emergent properties/functions in a myriad of cellular processes. Then, we focus on ENS of man-made (synthetic) molecules in cell-free conditions, classified by the types of enzymes. After that, we introduce the exploration of ENS of man-made molecules in the context of cells by discussing intercellular, peri/intracellular, and subcellular ENS for cell morphogenesis, molecular imaging, cancer therapy, and other applications. Finally, we provide a perspective on the promises of ENS for developing molecular assemblies/processes for functions. This review aims to be an updated introduction for researchers who are interested in exploring noncovalent synthesis for developing molecular science and technologies to address societal needs.

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DEVD-Based Hydrogelator Minimizes Cellular Apoptosis Induction

Cited by 19 publications

References 38 publications

Assembly of an Injectable Noncytotoxic Peptide-Based Hydrogelator for Sustained Release of Drugs

Assembly of an Injectable Noncytotoxic Peptide-Based Hydrogelator for Sustained Release of Drugs

Supramolecular Hydrogelators and Hydrogels: From Soft Matter to Molecular Biomaterials

Enzymatic Noncovalent Synthesis

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