Detoxification of sugarcane bagasse hemicellulosic hydrolysate with ion‐exchange resins for xylitol production by calcium alginate‐entrapped cells

Carvalho, Walter; Canilha, Larissa; Mussatto, Solange I.; Dragone, Giuliano; Morales, Martha Lissete Villarreal; Solenzal, A. I. Nápoles

doi:10.1002/jctb.1061

Cited by 41 publications

(23 citation statements)

References 23 publications

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“…Some physical-chemical methods of detoxification used in hemicellulosic hydrolysate treatment showed less favorable results in the removal of these compounds when compared to the method used in this work. According to Carvalho et al [23], after detoxification of hemicellulosic hydrolysate of sugarcane bagasse with four different types of resins (anion A-103 S, anion S-860, Applexion cationic and anionic Applexion), the authors observed a furfural removal of 82.1 and 66.5% of 5-HMF. In a similar study using anionic resins at pH 10 to detoxify the hemicellulosic hydrolysates of wood, Larsson et al [24] observed a drop of 73% in furfural amount and 70% of 5-HMF concentration.…”

Section: Resultsmentioning

confidence: 99%

Biological detoxification of different hemicellulosic hydrolysates using Issatchenkia occidentalis CCTCC M 206097 yeast

Fonseca

Moutta

Ferraz

et al. 2010

J Ind Microbiol Biotechnol

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This work had as its main objective to contribute to the development of a biological detoxification of hemicellulose hydrolysates obtained from different biomass plants using Issatchenkia occidentalis CCTCC M 206097 yeast. Tests with hemicellulosic hydrolysate of sugarcane bagasse in different concentrations were carried out to evaluate the influence of the hydrolysate concentration on the inhibitory compounds removal from the sugarcane bagasse hydrolysate, without reduction of sugar concentration. The highest reduction values of inhibitors concentration and less sugar losses were observed when the fivefold concentrated hydrolysate was treated by the evaluated yeast. In these experiments it was found that the high sugar concentrations favored lower sugar consumption by the yeast. The highest concentration reduction of syringaldehyde (66.67%), ferulic acid (73.33%), furfural (62%), and 5-HMF (85%) was observed when the concentrated hydrolysate was detoxified by using this yeast strain after 24 h of experimentation. The results obtained in this work showed the potential of the yeast Issatchenkia occidentalis CCTCC M 206097 as detoxification agent of hemicellulosic hydrolysate of different biomass plants.

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Section: Resultsmentioning

confidence: 99%

Biological detoxification of different hemicellulosic hydrolysates using Issatchenkia occidentalis CCTCC M 206097 yeast

Fonseca

Moutta

Ferraz

et al. 2010

J Ind Microbiol Biotechnol

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show abstract

“…In order to reduce the concentrations of inhibitory compounds found in hemicellulosic hydrolysates, and thus improve the bioconversion of sugars into desired products, different methods like pH adjustment [15,16], active charcoal adsorption [17,18], and ion-exchange resins adsorption [19,20], either alone or in combination [21,22], have been proposed as detoxification strategies.…”

Section: Introductionmentioning

confidence: 99%

Ethanol Production from Sugarcane Bagasse Hydrolysate Using Pichia stipitis

Canilha

Carvalho

Felipe

et al. 2009

Appl Biochem Biotechnol

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The objective of this study was to evaluate the ethanol production from the sugars contained in the sugarcane bagasse hemicellulosic hydrolysate with the yeast Pichia stipitis DSM 3651. The fermentations were carried out in 250-mL Erlenmeyers with 100 mL of medium incubated at 200 rpm and 30 degrees C for 120 h. The medium was composed by raw (non-detoxified) hydrolysate or by hydrolysates detoxified by pH alteration followed by active charcoal adsorption or by adsorption into ion-exchange resins, all of them supplemented with yeast extract (3 g/L), malt extract (3 g/L), and peptone (5 g/L). The initial concentration of cells was 3 g/L. According to the results, the detoxification procedures removed inhibitory compounds from the hemicellulosic hydrolysate and, thus, improved the bioconversion of the sugars into ethanol. The fermentation using the non-detoxified hydrolysate led to 4.9 g/L ethanol in 120 h, with a yield of 0.20 g/g and a productivity of 0.04 g L(-1) h(-1). The detoxification by pH alteration and active charcoal adsorption led to 6.1 g/L ethanol in 48 h, with a yield of 0.30 g/g and a productivity of 0.13 g L(-1) h(-1). The detoxification by adsorption into ion-exchange resins, in turn, provided 7.5 g/L ethanol in 48 h, with a yield of 0.30 g/g and a productivity of 0.16 g L(-1) h(-1).

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“…The toxic compounds include hydroxymethylfurfural, furfural (compounds derived from hexoses and pentoses degradation, respectively), acetic acid (released from the hemicellulosic structure) and phenolics (formed from the partial degradation of lignin) 4. To minimize the negative effects of these compounds on fermentation, numerous methodologies have been tested for hydrolysate treatment, including neutralisation,10, 11 evaporation12, 13 and adsorption on activated charcoal14, 15 or ion‐exchange resins 16, 17. Nevertheless, the effectiveness of a detoxification method depends on the type of hemicellulosic hydrolysate because each type of hydrolysate has a different degree of toxicity18 (which varies according to the raw material composition and conditions employed for hydrolysis).…”

Section: Introductionmentioning

confidence: 99%

Comparison of different procedures for the detoxification of eucalyptus hemicellulosic hydrolysate for use in fermentative processes

Carvalho¹,

Mussatto²,

Cândido³

et al. 2005

J of Chemical Tech & Biotech

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Eucalyptus (Eucalyptus grandis) shavings were submitted to an acid hydrolysis process with the aim of obtaining a hemicellulosic hydrolysate rich in fermentable sugars. However, the hydrolysate obtained contained, in addition to sugars, several compounds that are toxic to microorganisms, namely furfural, hydroxymethylfurfural, acetic acid and phenolics. In order to produce a hydrolysate suitable for use in fermentative processes, several procedures were evaluated for hydrolysate detoxification, including concentration by vacuum evaporation and adsorption on activated charcoal, diatomaceous earths, ionexchange resin or adsorbent resin. Hydrolysate concentration was especially effective for furfural removal, whereas the adsorbent resin was efficient in removing hydroxymethylfurfural, phenolics and acetic acid. Combination of this resin with activated charcoal was better than with diatomaceous earths for removal of acetic acid and phenolics. The best detoxification procedure evaluated was based on hydrolysate concentration followed by adsorption on activated charcoal and adsorbent resin. By this treatment, removal rates of 82.5, 100, 100 and 94% were attained for acetic acid, furfural, hydroxymethylfurfural and phenolics, respectively.

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Detoxification of sugarcane bagasse hemicellulosic hydrolysate with ion‐exchange resins for xylitol production by calcium alginate‐entrapped cells

Abstract: and a volumetric productivity (Q p ) of 0.24 g dm −3 h −1 were attained in the fermentation process for xylitol production from detoxified hydrolysate.

Cited by 41 publications

References 23 publications

Biological detoxification of different hemicellulosic hydrolysates using Issatchenkia occidentalis CCTCC M 206097 yeast

Biological detoxification of different hemicellulosic hydrolysates using Issatchenkia occidentalis CCTCC M 206097 yeast

Ethanol Production from Sugarcane Bagasse Hydrolysate Using Pichia stipitis

Comparison of different procedures for the detoxification of eucalyptus hemicellulosic hydrolysate for use in fermentative processes

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