Comparison of different procedures for the detoxification of eucalyptus hemicellulosic hydrolysate for use in fermentative processes

Carvalho, Giovani B. M.; Mussatto, Solange I.; Cândido, Elisângela J.; Silva, João Batista de Almeida e

doi:10.1002/jctb.1372

Cited by 67 publications

(58 citation statements)

References 29 publications

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“…These results are in agreement with literature data, since those indicate that the conditions of acid hydrolysis employed are effective to obtain D-xylose from the hemicellulose fraction, which can be used as a carbon source in fermentation processes [8,12].…”

Section: Resultssupporting

confidence: 93%

“…This knowledge not only helps to choose an efficient and low-cost treatment but also to establish conditions for hydrolysis that minimize its formation. Detoxification treatment with ion-exchange resin [12], activated charcoal [13], organic solvents [14], and overliming [9] are physical and chemical methods that are commonly used for reduction of all or part of these compounds formed after acid hydrolysis. Therefore many disadvantages still exist in these procedures for their use in technical applications.…”

Section: Introductionmentioning

confidence: 99%

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Biological detoxification of different hemicellulosic hydrolysates using Issatchenkia occidentalis CCTCC M 206097 yeast

Fonseca

Moutta

Ferraz

et al. 2010

J Ind Microbiol Biotechnol

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This work had as its main objective to contribute to the development of a biological detoxification of hemicellulose hydrolysates obtained from different biomass plants using Issatchenkia occidentalis CCTCC M 206097 yeast. Tests with hemicellulosic hydrolysate of sugarcane bagasse in different concentrations were carried out to evaluate the influence of the hydrolysate concentration on the inhibitory compounds removal from the sugarcane bagasse hydrolysate, without reduction of sugar concentration. The highest reduction values of inhibitors concentration and less sugar losses were observed when the fivefold concentrated hydrolysate was treated by the evaluated yeast. In these experiments it was found that the high sugar concentrations favored lower sugar consumption by the yeast. The highest concentration reduction of syringaldehyde (66.67%), ferulic acid (73.33%), furfural (62%), and 5-HMF (85%) was observed when the concentrated hydrolysate was detoxified by using this yeast strain after 24 h of experimentation. The results obtained in this work showed the potential of the yeast Issatchenkia occidentalis CCTCC M 206097 as detoxification agent of hemicellulosic hydrolysate of different biomass plants.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Biological detoxification of different hemicellulosic hydrolysates using Issatchenkia occidentalis CCTCC M 206097 yeast

Fonseca

Moutta

Ferraz

et al. 2010

J Ind Microbiol Biotechnol

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“…Also, Furfural concentration might be reduced due to its volatile nature. Our results showed resemblance with the report of Parajó et al [19] and Carvalho et al [20]. Detoxification results of hdrolysates by four strains i.e.…”

Section: Discussionsupporting

confidence: 92%

“…The concentration of acetic acid was increased, but not according to concentration factor used, which may be related, in part, to the volatility of it. Phenolics and 5-HMF concentrations showed the same behavior, suggesting a partial volatilization and/or degradation of 5-HMF [20]. Also, Furfural concentration might be reduced due to its volatile nature.…”

Section: Discussionmentioning

confidence: 82%

Screening of Yeasts for Selection of Potential Strains and Their Utilization for In Situ Microbial Detoxification (ISMD) of Sugarcane Bagasse Hemicellulosic Hydrolysate

et al. 2016

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Many toxic compounds are produced and released in the hemicellulosic hydrolyzates during the acid pretreatment step, which are required for the disruption of the lignocelluloses matrix and sugars release. The conventional methods of detoxification i.e. overliming, activated charcoal, ion exchange or even membrane-based separations have the limitations in removal of these toxic inhibitors in fermentation process. Hence, it is imperative to explore biological methods to overcome the inhibitors by minimizing the filtration steps, sugar loss and chemical additions. In the present study we screened sixty-four strains of yeasts to select potential strains for detoxification of furfural, acetic acid, ferulic acid, 5-hydroxymethyl furfural (5-HMF) as carbon and energy source. Among these strains Pichia occidentalis M1, Y1 0 a, Y1 0 b and Y3 0 showed a significant decrease in the toxic compounds but we selected two best yeast strains i.e. P. occidentalis Y1 0 a and P. occidentalis M1 for the further experiments with an aim to remove the fermentation inhibitors. The yeasts P. occidentalis Y1 0 a and P. occidentalis M1 were grown aerobically in sugarcane bagasse hemicellulose hydrolysate under submerged cultivation. For each yeast, a 2 2 full factorial design was performed considering the variables-pH (4.0 or 5.0) and agitation rate (100 or 300 rpm), and the percentage removal of HMF, furfural, acetic acid and phenols from hemicellulosic hydrolysates were responsive variables. After 96 h of biological treatment, P. occidentalis M1 and P. occidentalis Y1 0 a showed 42.89 and 46.04 % cumulative removal of inhibitors, respectively.

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“…However, even if several methods are being deemed to be the best effective, such as concentration by evaporation [9], activated charcoal adsorption [10], overlime [11], cation-and anion-exchange resins [12], or their combination [13,14], all have limitations. In general, they produce wastes and are costly; furthermore, the hydrolysate cannot reach the same fermentability degree of a synthetic pure medium [15].…”

Section: Introductionmentioning

confidence: 99%

Novel Isolates for Biological Detoxification of Lignocellulosic Hydrolysate

Hou-rui

Xiang-Xiang

Silva

et al. 2008

Appl Biochem Biotechnol

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In this paper, two new strians, Issatchenkia occidentalis (Lj-3, CCTCC M 2006097) and Issatchenkia orienalis (S-7, CCTCC M 2006098), isolated from different environments on solid media, were used in the detoxification process of the hemicellulosic hydrolysate of sugarcane bagasse. High-pressure liquid chromatography elution curve of UV-absorption compounds represented by acetic acid, furfural, and guaiacol (toxic compounds found in the hemicellulosic hydrolysate) showed that several chromatographic peaks were evidently diminished for the case of detoxified hydrolysate with isolate strains compared to the high peaks resulted for no detoxified hydrolysate. It was clear that these inhibitors were degraded by the two new isolates during their cultivation process. Fermentation results for the biodetoxified hydrolysate showed an increase in xylitol productivity (Q (p)) by 1.97 and 1.95 times (2.03 and 2.01 g l(-1) h(-1)) and in xylitol yield (Y (p)) by 1.72 and 1.65 times (0.93 and 0.89 g xylitol per gram xylose) for hydrolysate treated with S-7 and Lj-3, respectively, in comparison with no detoxified hydrolysate (1.03 g l(-1) h(-1) and 0.54 g xylitol per gram xylose). This present work demonstrated the importance of Issatchenkia yeast in providing an effective biological detoxification approach to remove inhibitors and improve hydrolysate fermentability, leading to a high xylitol productivity and yield.

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Comparison of different procedures for the detoxification of eucalyptus hemicellulosic hydrolysate for use in fermentative processes

Cited by 67 publications

References 29 publications

Biological detoxification of different hemicellulosic hydrolysates using Issatchenkia occidentalis CCTCC M 206097 yeast

Biological detoxification of different hemicellulosic hydrolysates using Issatchenkia occidentalis CCTCC M 206097 yeast

Screening of Yeasts for Selection of Potential Strains and Their Utilization for In Situ Microbial Detoxification (ISMD) of Sugarcane Bagasse Hemicellulosic Hydrolysate

Novel Isolates for Biological Detoxification of Lignocellulosic Hydrolysate

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