Detoxification of Lignocellulose Hydrolysates: Biochemical and Metabolic Engineering Toward White Biotechnology

Chandel, Anuj K.; Silva, Sílvio Silvério da; Singh, Om V.

doi:10.1007/s12155-012-9241-z

Cited by 192 publications

(110 citation statements)

References 86 publications

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“…The precise mechanism of furfural toxicity is unknown. Although methods have been developed to mitigate or remove in-hibitors (23), all of them increase process complexity. Furfural has been shown to cause strand breaks in DNA (24), to damage membranes (25), and to react with other cellular components (10,26).…”

mentioning

confidence: 99%

Polyamine Transporters and Polyamines Increase Furfural Tolerance during Xylose Fermentation with Ethanologenic Escherichia coli Strain LY180

Geddes

Wang

Yomano

et al. 2014

Appl Environ Microbiol

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Expression of genes encoding polyamine transporters from plasmids and polyamine supplements increased furfural tolerance (growth and ethanol production) in ethanologenic Escherichia coli LY180 (in AM1 mineral salts medium containing xylose). This represents a new approach to increase furfural tolerance and may be useful for other organisms. Microarray comparisons of two furfural-resistant mutants (EMFR9 and EMFR35) provided initial evidence for the importance of polyamine transporters. Each mutant contained a single polyamine transporter gene that was upregulated over 100-fold (microarrays) compared to that in the parent LY180, as well as a mutation that silenced the expression of yqhD. Based on these genetic changes, furfural tolerance was substantially reconstructed in the parent, LY180. Deletion of potE in EMFR9 lowered furfural tolerance to that of the parent. Deletion of potE and puuP in LY180 also decreased furfural tolerance, indicating functional importance of the native genes. Of the 8 polyamine transporters (18 genes) cloned and tested, half were beneficial for furfural tolerance (PotE, PuuP, PlaP, and PotABCD). Supplementing AM1 mineral salts medium with individual polyamines (agmatine, putrescine, and cadaverine) also increased furfural tolerance but to a smaller extent. In pH-controlled fermentations, polyamine transporter plasmids were shown to promote the metabolism of furfural and substantially reduce the time required to complete xylose fermentation. This increase in furfural tolerance is proposed to result from polyamine binding to negatively charged cellular constituents such as nucleic acids and phospholipids, providing protection from damage by furfural.

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confidence: 99%

Polyamine Transporters and Polyamines Increase Furfural Tolerance during Xylose Fermentation with Ethanologenic Escherichia coli Strain LY180

Geddes

Wang

Yomano

et al. 2014

Appl Environ Microbiol

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“…Among those inhibitors, furfural was found to be able to inactivate the cell replication by breaking down the single-strand [31][32][33][34]. Organic acid (acetic acid) derived from hemicellulose could cross the cell membrane, which resulted in the lower cell pH than normal and consequently inhibited cell activity [33,35]. Ultimately, the cell activity of E. coli was inhibited and directly reduced its fermentation ability.…”

Section: Effect Of Detoxification On Isoprenoids Productionmentioning

confidence: 99%

Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using the Engineered <em>E. coli</em>

Wang¹,

Yang²

2017

Preprint

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Microalgae are recognized as a third generation feedstock for biofuel production due to its rapid growth rate and lignin-free characteristic. In this study, the lipid extracted microalgal biomass residues was used as the material to produce isoprene, α-pinene and β-pinene with the engineered E. coli strain. We adopted an optimal sulfuric acid hydrolysis method (1:7 ratio of solid to acid solution, 32 % (w/v) concentration of sulfuric acid solution at 90℃ for 90 min) to convert holocellulose into glucose efficiently (6.37 g/L) and explored a novel detoxification strategy (phosphoric acid/calcium hydroxide) to remove inhibitors notably. 55.32 % acetic acid, 99.19 % furfural and 98.22 % 5-HMF (5-hydroxymethylfurfural) were cut down with the phosphoric acid/calcium hydroxide method, and the fermentation concentration of isoprene (223.23 mg/L), α-pinene (382.21 μg/L) and β-pinene (17.4 mg/L) using the detoxified hydrolysate as the carbon source account for approximately 86.02 %, 90.16 % and 88.32 % of those produced by the engineered E. coli strain fermented on pure glucose, respectively.

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“…One of the main challenges associated with 2G ethanol production is to overcome the cumulative negative effect of cell wall derived inhibitors on the fermenting microorganisms during fermentation reactions [7][8][9][10][11]. Inhibitory compounds like acids, furans and phenolic compounds interferes the fermenting microorganisms and complete process by reducing ethanol yield and productivity during fermentation.…”

Section: Introductionmentioning

confidence: 99%

“…Biological methods certainly have unique advantages. Further, to consolidate the detoxification and ethanol or xylitol fermentation so called simultaneous detoxification and fermentation (SDF) is also possible by the appropriate use of microorganisms in a single vessel [11].…”

Section: Introductionmentioning

confidence: 99%

Screening of Yeasts for Selection of Potential Strains and Their Utilization for In Situ Microbial Detoxification (ISMD) of Sugarcane Bagasse Hemicellulosic Hydrolysate

et al. 2016

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Many toxic compounds are produced and released in the hemicellulosic hydrolyzates during the acid pretreatment step, which are required for the disruption of the lignocelluloses matrix and sugars release. The conventional methods of detoxification i.e. overliming, activated charcoal, ion exchange or even membrane-based separations have the limitations in removal of these toxic inhibitors in fermentation process. Hence, it is imperative to explore biological methods to overcome the inhibitors by minimizing the filtration steps, sugar loss and chemical additions. In the present study we screened sixty-four strains of yeasts to select potential strains for detoxification of furfural, acetic acid, ferulic acid, 5-hydroxymethyl furfural (5-HMF) as carbon and energy source. Among these strains Pichia occidentalis M1, Y1 0 a, Y1 0 b and Y3 0 showed a significant decrease in the toxic compounds but we selected two best yeast strains i.e. P. occidentalis Y1 0 a and P. occidentalis M1 for the further experiments with an aim to remove the fermentation inhibitors. The yeasts P. occidentalis Y1 0 a and P. occidentalis M1 were grown aerobically in sugarcane bagasse hemicellulose hydrolysate under submerged cultivation. For each yeast, a 2 2 full factorial design was performed considering the variables-pH (4.0 or 5.0) and agitation rate (100 or 300 rpm), and the percentage removal of HMF, furfural, acetic acid and phenols from hemicellulosic hydrolysates were responsive variables. After 96 h of biological treatment, P. occidentalis M1 and P. occidentalis Y1 0 a showed 42.89 and 46.04 % cumulative removal of inhibitors, respectively.

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Detoxification of Lignocellulose Hydrolysates: Biochemical and Metabolic Engineering Toward White Biotechnology

Cited by 192 publications

References 86 publications

Polyamine Transporters and Polyamines Increase Furfural Tolerance during Xylose Fermentation with Ethanologenic Escherichia coli Strain LY180

Polyamine Transporters and Polyamines Increase Furfural Tolerance during Xylose Fermentation with Ethanologenic Escherichia coli Strain LY180

Isoprenoids Production from Lipid-Extracted Microalgal Biomass Residues Using the Engineered <em>E. coli</em>

Screening of Yeasts for Selection of Potential Strains and Their Utilization for In Situ Microbial Detoxification (ISMD) of Sugarcane Bagasse Hemicellulosic Hydrolysate

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