Detoxification of aflatoxins by seed extracts of the medicinal plant, Trachyspermum ammi (L.) Sprague ex Turrill – Structural analysis and biological toxicity of degradation product of aflatoxin G1

Velazhahan, R.; Selvaraj, Vijayanandraj; Vijayasamundeeswari, Ayyathurai; Paranidharan, V.; Samiyappan, R.; Iwamoto, Takeo; Friebe, Bernd; Muthukrishnan, Subbaratnam

doi:10.1016/j.foodcont.2009.10.014

Cited by 95 publications

(72 citation statements)

References 33 publications

(33 reference statements)

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“…Hence there is an urgent need to develop or investigate the biological methods which prevent the production of aflatoxin or prevents activity. It is well established that several authors reported that some medicinal plants have the potential to destroy aflatoxin [9,25]. There were several reports of higher levels of aflatoxins in the cultivated groundnut based cattle and poultry feeds.…”

Section: Molecular Identification Of Isolated Strainmentioning

confidence: 99%

Inhibition of Aspergillus flavus growth and detoxification of Aflatoxin B1 by the medicinal plant leaf extract- Cymbopogon citratus

2016

IJCBS

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Aflatoxin contamination in food is a major qualititative problem affecting human health and trade. Detoxification of these aflatoxins has been considered as one of the best strategies to decontaminate the food and animal feed. The aim of the present study was to isolate, identify and detoxify the aflatoxin produced by A.flavus from infected ground nut. The present study was focused on isolation, identification of A. flavus from infected groundnut pods on Czapek Dox Agar medium, and the toxigenic strains were confirmed on Coconut Cream Agar Medium (CCA). Aqueous extracts of C. citratus were tested against aflatoxin producing strains of A. flavus at various concentrations (1, 2.5 and 5% v/v). Further, the detoxifying capacity of these leaf extracts was studied at these concentrations on AFB1 using TLC and HPLC. The toxigenic strain of A. flavus was confirmed based on PCR amplification. Results indicated that C. citratus extracts exhibited high antifungal activity showing inhibition of fungal mycelium to a maximum extent at 5% v/v concentration. However, the other two concentrations of C. citratus were also found to be effective in inhibition of A. flavus mycelia. Detoxification studies indicated lesser levels of AFB1 at all the concentrations under study. Maximum degradation of the toxin was obtained at 5% v/v concentration from 2154 μg/L to 183 μg/L. It was observed that the extract of C. citratus showed significant detoxification and inhibitory effect of growth of A. flavus. Our future studies are directed in characterizing the active principle of the C. citratus plant extract and application of these extracts on detoxification of Aflatoxins.

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Section: Molecular Identification Of Isolated Strainmentioning

confidence: 99%

Inhibition of Aspergillus flavus growth and detoxification of Aflatoxin B1 by the medicinal plant leaf extract- Cymbopogon citratus

2016

IJCBS

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“…These investigators further demonstrated that a recombinant Escherichia coli expressing zhd101 completely inactivated zearalenone and zearalenol within 1 h. It has been demonstrated that transgenic maize plants expressing the detoxification gene, zhd101 showed reduced contamination by the mycotoxin, zearalenone in maize kernels [Igawa et al, 2007]. Recently studies have been focused on identification of the aflatoxin detoxification genes from Trachyspermum ammi and to transfer them into crop plants in order to develop transgenic resistance to aflatoxin contamination [Velazhahan et al, 2010].…”

Section: Biotechnological Approaches For Fighting Aflatoxin-producingmentioning

confidence: 99%

“…Though some of the fungicides are effective in preventing the growth of Aspergillus flavus in storage especially as a fumigant [Paster et al, 1995], consumer concerns about possible risks associated with the use of fungicides have resulted in an intensive search for safer and more effective control options that pose minimal risk to human health and the environment. [Velazhahan et al, 2010,]. Whichever decontamination strategy is used, it must meet some basic criteria [Park, 1993;Beaver, 1991;Pomeranz et al, 1990]:  The mycotoxin must be inactivated (destroyed) by transformation to non-toxic compounds.…”

Section: Introductionmentioning

confidence: 99%

“…Significant levels of degradation of other aflatoxins viz., aflatoxin B1 (61%), aflatoxin B2 (54%) and aflatoxin G2 (46%) by the dialyzed Trachyspermum ammi extract was also observed. Time course study of aflatoxin G1 detoxification by dialyzed Trachyspermum ammi extract showed that more than 78% degradation occurred within 6 h and 91% degradation occurred 24 h after incubation [Velazhahan et al, 2010]. Other plant extracts namely, Syzigium aromaticum, Allium sativum, Curcuma longa, Ocimum sanctum, Annona squamosa, Azadirachta indica (Neem), Allium cepa, Eucalyptus terticornis, and Pongamia glaberima are among the list of the plant extracts in inhibiting both fungal growth and aflatoxin production by Aspergillus [Reddy et al, 2009].…”

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confidence: 99%

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Phytoinhibition of Growth and Aflatoxin Biosynthesis in Toxigenic Fungi

Allameh¹,

Ziglari²,

Rasooli³

2011

Aflatoxins - Detection, Measurement and Control

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“…A number of biological actions have been claimed for T. ammi fruits as antispasmodic, stimulant, tonic, digestive, anthelmintic, anticipative, antimicrobial, antiviral, antifilarial, anti-inflammatory, antipyretic, antinociceptive, antifungal, analgesic diuretic and carminative effects, as indicated the various authors and traditional medicine uses. The seeds of this plant contain about 2-4% essential oil that have a biological activity 2,[9][10][11][12][13][14] . The T. ammi seeds known as Nakhwa or Nankha in Arabic countries and used mainly as a spice and in many folk medicine uses.…”

Section: Introductionmentioning

confidence: 99%

Chemical composition of volatile components, antimicrobial and anticancer activity of n-hexane extract and essential oil from Trachyspermum ammi L. seeds

Abdel‐Hameed¹,

Bazaid²,

Zahrani³

et al. 2014

Orient J Chem

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The aim of this study was to characterize the chemical composition of some volatile components, in vitro antimicrobial and anticancer activity of essential oil and n-hexane extract from Trachyspermum ammi L. (Family Apiaceae). The chemical composition of samples was obtained by GC-MS analysis, the antimicrobial activity was evaluated by disc diffusion method whereas the in vitro anticancer activity was evaluated by sulphorhodamine method. Twenty-three monoterpenoide compounds were identified in the essential oil in which four compounds; -terpinene, thymol, P-cymene and -pinene were the major components of the oil with quantity 266. 28, 201.97, 194.91 and 38.49 mg/g oil respectively whereas the other nineteen compounds had quantity < 10 mg/g oil. Twelve monoterpene compounds were identified in the n-hexane extract in which three compounds. Thymol, -terpinene and P-cymene were the major components of volatile components of the n-hexane extract with quantity 138.85, 56.41 and 32.69 mg/g extract respectively whereas the other nine compounds had quantity < 10 mg/g extract. The essential oil and n-hexane extract exhibited an antimicrobial activity against five microorganisms and an anticancer activity against HepG2. The essential oil showed higher activity than the n-hexane. Thymol, terpinene and P-cymene of the two samples play an important role in antimicrobial and anticancer activity. In conclusion, this considered the first report that gave the real quantity of each volatile compound in the essential oil and n-hexane extract of T. ammi. Also, this the first work dealing with the anticancer activity of the two samples in addition to the agreement of antimicrobial activity with previous studies. More safety and toxicological studies will need to be addressed if the essential oil and n-hexane extract of T. ammi are to be used for food preservation or medicinal purposes.

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Detoxification of aflatoxins by seed extracts of the medicinal plant, Trachyspermum ammi (L.) Sprague ex Turrill – Structural analysis and biological toxicity of degradation product of aflatoxin G1

Cited by 95 publications

References 33 publications

Inhibition of Aspergillus flavus growth and detoxification of Aflatoxin B1 by the medicinal plant leaf extract- Cymbopogon citratus

Inhibition of Aspergillus flavus growth and detoxification of Aflatoxin B1 by the medicinal plant leaf extract- Cymbopogon citratus

Phytoinhibition of Growth and Aflatoxin Biosynthesis in Toxigenic Fungi

Chemical composition of volatile components, antimicrobial and anticancer activity of n-hexane extract and essential oil from Trachyspermum ammi L. seeds

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