Detoxification and synergism of diflubenzuron and chlorfluazuron in the red flour beetle Tribolium castaneum

Gazit, Yoav; Ishaaya, I.; Perry, A. S.

doi:10.1016/0048-3575(89)90147-8

Cited by 25 publications

(18 citation statements)

References 16 publications

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“…By feeding T. castaneum larvae a diet with DFB for three days, we were able to confirm the toxic effects described previously for this species (Gazit et al, 1989). The LC 50 values for DFB were reported previously to be approximately 13 ppm for fourth instar larvae until pupation and 11 ppm until emergence.…”

Section: Discussionsupporting

confidence: 81%

Genomic and proteomic studies on the effects of the insect growth regulator diflubenzuron in the model beetle species Tribolium castaneum

Merzendorfer

Kim

Chaudhari

et al. 2012

Insect Biochemistry and Molecular Biology

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SUMMARY Several benzoylphenyl urea-derived insecticides such as diflubenzuron (DFB, Dimilin®) are in wide use to control various insect pests. Although this class of compounds is known to disrupt molting and to affect chitin content, their precise mode of action is still not understood. To gain a broader insight into the mechanism underlying the insecticidal effects of benzoylphenyl urea compounds, we conducted a comprehensive study with the model beetle species and stored product pest Tribolium castaneum (red flour beetle) utilizing genomic and proteomic approaches. DFB was added to a wheat flour-based diet at various concentrations and fed to larvae and adults. We observed abortive molting, hatching defects and reduced chitin amounts in the larval cuticle, the peritrophic matrix and eggs. Electron microscopic examination of the larval cuticle revealed major structural changes and a loss of lamellate structure of the procuticle. We used a genomic tiling array for determining relative expression levels of about 11,000 genes predicted by the GLEAN algorithm. About 6% of all predicted genes were more than 2-fold up-or down-regulated in response to DFB treatment. Genes encoding enzymes involved in chitin metabolism were unexpectedly unaffected, but many genes encoding cuticle proteins were affected. In addition, several genes presumably involved in detoxification pathways were up-regulated. Comparative 2D gel electrophoresis of proteins extracted from the midgut revealed 388 protein spots, of which 7% were significantly affected in their levels by DFB treatment as determined by laser densitometry. Mass spectrometric identification revealed that UDP-N-acetylglucosamine pyrophosphorylase and glutathione synthetase were up-regulated. In summary, the red flour beetle turned out to be a good model organism for investigating the global effects of bioactive materials such as insect growth regulators and other insecticides. The results of this study recapitulate all of the different DFB-induced symptoms in a single model insect, which have been previously found in several different insect species, and further illustrate that DFB-treatment causes a wide range of effects at the molecular level.

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Section: Discussionsupporting

confidence: 81%

Genomic and proteomic studies on the effects of the insect growth regulator diflubenzuron in the model beetle species Tribolium castaneum

Merzendorfer

Kim

Chaudhari

et al. 2012

Insect Biochemistry and Molecular Biology

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“…Similar results were obtained with piperonyl butoxide and S,S,S-tributylphosphorotrithioate on other populations of P. xylostella from Serdang (Furlong & Wright, 1994) and a highland region of Malaysia (Fauziah & Wright, 1992). More direct evidence for the involvement of oxidative mechanisms in acylurea resistant populations of P. xylostella from Taiwan has been reported by Sun (1992), while the involvement of hydrolytic mechanisms in the detoxification of acylureas has been observed in other insect species (El Saidy et al, 1989;Gazit et al, 1989;Ishaaya & Klein, 1990;Van Laecke & Degheele, 1991a,b).…”

Section: Discussionmentioning

confidence: 89%

Evaluation of resistance, cross-resistance and synergism of abamectin and teflubenzuron in a multi-resistant field population of Plutella xylostella (Lepidoptera: Plutellidae)

Iqbal

Wright

1997

Bull. Entomol. Res.

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The efficacy of abamectin (Agrimec") and teflubenzuron (Nomolt") was assessed by leaf-dip bioassay against larvae of the diamondback moth, Plutella xylostella Linnaeus from a population (SERD3) collected originally in lowland Malaysia in December 1994. Evidence for resistance to both abamectin and teflubenzuron was found in the F 7 generation (LC 50 ratio of 60 and 24 respectively compared with a laboratory, insecticide-susceptible strain). Selection of sub-populations of SERD3 (F^F 9 ) with abamectin and teflubenzuron increased the LC 50 ratio to 220 and 360 respectively and estimates of realized heritability [h 2 ] were high (c. 0.8 and 0.9) for both compounds. There was no cross-resistance between these compounds in the abamectin and teflubenzuron-selected sub-populations but some indication of negatively-correlated resistance. Topical application of the synergists piperonyl butoxide, S,S,S-tributylphosphorotrithioate and maleic acid diethyl ester to the laboratory strain had no significant effect on the toxicity of abamectin or teflubenzuron in subsequent leaf-dip assays. In contrast, pre-treatment with piperonyl butoxide and S,S,S-tributylphosphorotrithioate significantly increased the toxicity of abamectin (c. 4-and 3-fold) and teflubenzuron (c. 7-and 19-fold) in the abamectin and teflubenzuron-selected sub-populations of SERD3, suggesting that microsomal monoxygenases and /or esterases may be involved in resistance. Pre-treatment with maleic acid diethyl ester only increased the toxicity of abamectin by c. 2-fold and had no significant effect on the toxicity of teflubenzuron, providing limited evidence for the involvement of glutathione-S-transferases in resistance to the former compound alone.

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“…Synergism studies with teßubenzuron-resistant strains of Plutella xylostella have suggested that resistance may involve both monooxygenases and esterases (Furlong and Wright 1994). In addition, a number of studies on dißubenzuron detoxiÞcation in insecticide-susceptible strains of various species of Lepidoptera, Coleoptera and Diptera have shown that the principal pathway for metabolism involves hydrolysis and, in some cases, conjugation (Ivie and Wright 1978, Chang and Stokes 1979, Bull and Ivie 1980, El Saidy et al 1989, Gazit et al 1989, Van Laeke and Degheele 1991. It is most likely therefore, given the polygenic nature of the resistance, that one or more of these mechanisms contribute along with cytochrome P450 enzymes to the resistance shown by Dfbsel.…”

Section: Discussionmentioning

confidence: 97%

Inheritance of Diflubenzuron Resistance and Monooxygenase Activities in a Laboratory-Selected Strain of <I>Lucilia cuprina</I> (Diptera: Calliphoridae)

Kotze

Sales

2001

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Inheritance of the high-level diflubenzuron resistance shown by a laboratory-selected strain of Lucilia cuprina (Wiedemann) was examined in matings with a susceptible reference strain. Progeny of reciprocal crosses between resistant females and susceptible males showed higher LC50 values than the alternate reciprocal cross, indicating some maternal influence on inheritance of resistance. Resistance was inherited in a codominant (S male x R female) or incompletely recessive (R male x S female) manner. Monooxygenase activities (aldrin epoxidation) of the F1 generations were also intermediate between the levels shown by the parental lines, however, inheritance of enzyme activities showed greater degrees of dominance than for resistance levels. There was also some maternal influence on inheritance of monooxygenase activities. Backcrosses of F1 generations to both susceptible and resistant parents did not fit the expected patterns for a major sex-linked resistance locus, indicating that the maternal influence on resistance inheritance was not associated with sex-linkage of a major resistance gene. The backcross data also failed to fit the model for a single major autosomal gene, suggesting that the resistance in the diflubenzuron-selected strain is polygenic, involving mechanisms additional to monooxygenases.

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Detoxification and synergism of diflubenzuron and chlorfluazuron in the red flour beetle Tribolium castaneum

Cited by 25 publications

References 16 publications

Genomic and proteomic studies on the effects of the insect growth regulator diflubenzuron in the model beetle species Tribolium castaneum

Genomic and proteomic studies on the effects of the insect growth regulator diflubenzuron in the model beetle species Tribolium castaneum

Evaluation of resistance, cross-resistance and synergism of abamectin and teflubenzuron in a multi-resistant field population of Plutella xylostella (Lepidoptera: Plutellidae)

Inheritance of Diflubenzuron Resistance and Monooxygenase Activities in a Laboratory-Selected Strain of <I>Lucilia cuprina</I> (Diptera: Calliphoridae)

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