The difficulties and limitations associated with the available physicochemical methods for the determination of vitamin D in oil samples, including those containing vitamin A, led to the present investigation. The proposed method utilizes a two-step chromatographic process for the separation of vitamin D from the other nonsaponifiable oil components. In the first step an activated earth, Superfiltrol, is used to remove the vitamin A, related carotenoids, pigments, and some sterols.In the second step activated alumina is used to remove certain poly enes, as well as other residues from the oil and Superfiltrol itself. The vitamin D is ultimately determined by utilizing its absorption maximum at 265 mg as a measure