Determining the potential indigenous red-yeasts producing β-carotene and their phylogenetic relationship

Abstract

Red-yeasts are pigmented yeast species that could synthesize carotenoids for food supplements and pharmaceutical purposes. However, this group contains a lot of species which need to be explored thoroughly. The objective of this study was to view the β-carotene production of three indigenous red-yeasts by modifying glucose content in the growth medium and verifying their phylogenetic relationship. The glucose content modification in growth media influenced the β-carotene production of each species. Rhodosporidiobolus ruineniae (InaCCY1638/Y15Eg075) and Rhodosporidiobolus poonsokiae (InaCCY1606/Y15Kr068) produced higher β-carotene than Rhodotorula paludigena (InaCCY1527/Y15Eg004). These Rhodosporidiobolus species were able to produce higher β-carotene from 0.5 to 2% glucose content while Rhodotorula was low production in 2% glucose content. The higher producers by Rhodosporidiobolus species were clustered to Ruineniae clade and could be a potential clade for higher β-carotene production. Based on this result, using a high carotenoid producer of red-yeasts from indigenous strains is potential to be developed for β-carotene bioindustry in the future.

Biosynthesis and profiling of single cell carotenoids of Phaf ia rhodozyma in waste-based cultivation media

“…Carotenoids were extracted according the method of Sulistyo et al (2016), One mililitter of cell suspension was centrifuged at 10,000 x g, 4ºC for 10 min and washed twice with distilled water. Dried pellet was added with 2 mL dimethyl sulfoxide (DMSO) and 1 mL 1 M phosphate buffer (pH 7.0), and the homogenized in the 15 ml corning tube containing glass bead (5 mm in diameter).…”

“…Total carotenoid analysis (astaxanthin and β-carotene) were performed by highperformance liquid chromatography (HPLC) according to the method of Sulistyo et al (2016). HPLC (LC-20AB) (Shimadzu Co. Inc., Tokyo, Japan) was equipped with a Spherisorb ODS2 4.0µm by 250 x 4,6 mm C18 column (Luna 5u Silica (2) 100 Å) using a 15-min gradient of ethyl acetate (0 to 100%) in hexane-acetone (86:14, v/v) at a flow rate of 2 mL/min in 20 o C. Individual peaks of absorption spectra were obtained with a photodiode array detector with the typical retention times at λ 474 nm.…”