Determining sample size for the morphological assessment of sperm

Kuster, Christopher E.; Singer, R.; Althouse, Gary C.

doi:10.1016/s0093-691x(03)00240-1

Cited by 45 publications

(28 citation statements)

References 10 publications

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“…Sperm morphology was recommended to be one of the most essential qualitative characteristics of poultry semen (36). It could be used as an essential parameter for predicting the fertilizing ability of spermatozoa (37).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the ejaculate quality of the red jungle fowl, domestic chicken, and bantam chicken in Malaysia

Malik¹,

Haron²,

Yusoff³

et al. 2013

Turk J Vet Anim Sci

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Section: Discussionmentioning

confidence: 99%

Evaluation of the ejaculate quality of the red jungle fowl, domestic chicken, and bantam chicken in Malaysia

Malik¹,

Haron²,

Yusoff³

et al. 2013

Turk J Vet Anim Sci

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“…An understanding of the significance of specific types of sperm abnormalities and the numbers in which these are detected in the ejaculate allows the diagnostician to make a prognosis of fertility and might indicate a course of treatment to assist in the recovery of a male with abnormal sperm production (Freneau et al 2010). It should be noted that there is currently a substantial amount of support for the recommendation that the minimum percentage of morphologically normal sperm in an ejaculate should lie in the range of 70-80% in order to achieve optimum fertility (Kuster et al 2004). The current findings are consistent to the normal range of sperm morphology accepted for other species, since that 80% normal sperm were verified in the use of all the staining techniques in collared peccaries.…”

Section: Discussionmentioning

confidence: 99%

Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu)

et al. 2013

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Pesq. Vet. Bras. 33 (7) The aim of this study was to compare different staining methods for the evaluation of sperm morphology by light microscopy and also to describe the morphometry of the entire sperm in collared peccaries (Pecari tajacu). Semen from 10 males was obtained by electroejaculation and evaluated for sperm motility, vigor, and concentration. Semen smears were prepared through three different staining methods: Bengal rose, brome-phenol blue, and eosin-nigrosin. Smears were evaluated under light microscopy and sperm morphologic alterations were determined in percentage. In addition, sperm morphometric analysis was conducted by light microscopy coupled to image analyzer software. The smears stained with Bengal Rose provide the best results for the visualization of the sperm tail, midpiece, and head. The use of eosin-nigrosin stain did not allow an adequate impregnation, and some sperm presented a few contrasts with the background. A higher incidence of bent coiled tails was verified in the use of brome-phenol blue staining (P<0.05). Through morphometric evaluation, it was observed that the tail occupies the greatest proportion (89%) of the sperm which presents a discretely elongated head. According to the results, the use of the Bengal Rose stain is recommended for the morphologic evaluation of the collared peccary sperm.

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“…Morphology of spermatozoa is an important for a successful fertilization and early embryonic development in assisted reproductive techniques (Kuster et al, 2004). Final Tygerberg Classification Criteria described by Kruger in 1986 and the WHO classification are the most important spermatozoa morphological classifications.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric Measurements

et al. 2012

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AKSOY, E.; AKTAN, T. M.; DUMAN, S. & CUCE, G.Evaluación de la morfología de los espermatozoides bajo microscopía óptica con diferentes tinciones histológicas y comparación de las mediciones morfométricas. Int. J. Morphol., 30(4):1544-1550, 2012. SUMMARY:The aim was to examine the morphology of spermatozoa with different staining methods and aimed to find the better staining methods for morphology of spermatozoa in our study. Randomized 67 patients taken for the study who were admitted to Assisted Reproductive Techniques Unit. In the first part of the study, smears were stained with Hematoxylin Eosin (HE), Toluidin Blue (TB), Giemsa, Wright, ferrous Weigert haematoxylin stain, Orange G, eosin-aniline blue dye, Shorr Method, Papanicolau, Berg Method, Light Green stain, Acridine Orange (AO) and Janus Green dyes. In the second part of the study, smear preparations of 10 patients with normozoospermic were stained with HE, Toluidin Blue (TB), Shorr Method and Papanicolau. Four measurements were made including the middle piece, head length-head width and tail length for 200 spermatozoa with normal morphology. Comparisons were made between the stains that which showed a better morphology. Condensation assessment was not possible in smears stained with Shorr, Berg, Method, AO. Better assessment of condensation could be made in other stains. In the second part the smallest values belonged to of TB stain according to measurements of head of the spermatozoa. There was a significant difference at the head length with TB stain. Although measurements of Shorr and Papanicolau are close to each other and the largest values belonged to Papanicolau dye. It was concluded that measurement values in human sperm morphology could alter with the used staining method.

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Determining sample size for the morphological assessment of sperm

Cited by 45 publications

References 10 publications

Evaluation of the ejaculate quality of the red jungle fowl, domestic chicken, and bantam chicken in Malaysia

Evaluation of the ejaculate quality of the red jungle fowl, domestic chicken, and bantam chicken in Malaysia

Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu)

Assessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric Measurements

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