Determining mineral content variations in bone using backscattered electron imaging

Bloebaum, Roy D.; Skedros, John G.; Vajda, Eric G.; Bachus, Kent N.; Constantz, Brent R.

doi:10.1016/s8756-3282(97)00015-x

Cited by 151 publications

(120 citation statements)

References 30 publications

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“…In this study, the bone mineral density was measured by detecting backscattered electrons using an SEM. As shown by some others [5,35], this methods allows to measure the bone mineralization. However, it was not possible to determine if mineralization itself was impaired by the presence of zoledronate.…”

Section: Discussionmentioning

confidence: 80%

Calcium phosphate drug delivery system: influence of local zoledronate release on bone implant osteointegration

Peter

Pioletti

Laı̈b

et al. 2005

Bone

252

167

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Despite total hip replacement (THR) gives generally satisfactory results, the quality of outcome in young patients is markedly decreased compared to the average THR outcome. For this population, pharmacological treatment with bisphosphonate would be beneficial to decrease the peri-implant osteolysis. However, as this population does not necessarily suffer from osteoporosis, a nonsystemic treatment would be preferable. Zoledronate was then grafted to hydroxyapatite (HA) coating of titanium implants. The implants were inserted in rat condyles with various zoledronate concentrations. A positive concentration-dependent effect was observed on the peri-implant bone density and on different histomorphometric parameters. Importantly for the outcome of the implants, the mechanical fixation was increased by the local presence of zoledronate.The obtained results open the way of an easy transformation of currently existing HA-coated implants by grafting bisphosphonate onto the coating in order to increase their service life in the patients. D 2004 Elsevier Inc. All rights reserved.

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Section: Discussionmentioning

confidence: 80%

Calcium phosphate drug delivery system: influence of local zoledronate release on bone implant osteointegration

Peter

Pioletti

Laı̈b

et al. 2005

Bone

252

167

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“…The greater trochanters of the rats were sectioned with a smaller diamondimpregnated blade using a water-cooled bone saw (Isomet; Beuler, Lake Bluff, IL). One coronal section from each specimen was used for light histology of the tendon insertion, and the other coronal section was used for quantitative BSE imaging of the calcified fibrocartilage and cortical bone (Skedros et al, 1993a;Bloebaum et al, 1997;Roschger et al, 1998).…”

Section: Materials and Methods Specimen Preparationmentioning

confidence: 99%

“…Recent advances in backscattered electron (BSE) technology have enabled researchers to examine the mineral content of bone independent of porosity on a microscopic scale (Vajda et al, 1995;Bloebaum et al, 1997;Roschger et al, 1998). The main objective of the present study was to begin the process of validating an animal model for examining the healing and mechanical properties of calcified fibrocartilage.…”

mentioning

confidence: 99%

Experimental confirmation of the sheep model for studying the role of calcified fibrocartilage in hip fractures and tendon attachments

Shea¹,

Hallows²,

Bloebaum³

2002

Anat. Rec.

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Research has shown that there is a dramatic increase in the fractional area of calcified fibrocartilage from tendon and capsular insertions on the human femoral neck Shea et al., 2001b). Additional information regarding the properties of the proximal femur's cortical shell, gained from the use of an animal model, may result in a better understanding of elderly hip fracture since the cortical shell is a significant contributor to the strength of the proximal femur. The objective of the present study was to determine if the greater trochanter's tendon insertions of the human, rat, and sheep differ in terms of morphology and mineralization. The tendons of the greater trochanter of the human, rat, and sheep were observed to insert via a fibrocartilage insertion. The mineral content of the human and sheep calcified fibrocartilage was significantly higher than that of the rat calcified fibrocartilage (P Ͻ 0.01). Additionally, the mineral content of the rat cortical bone was significantly higher than that of the human cortical bone (P Ͻ 0.01). The mineral content of the calcified fibrocartilage and bone of the human and sheep were not statistically different from each other. There were also more similarities between the bone structure and lacunae density of the human and sheep than between the human and the rat. This suggests that the tendon insertions of the sheep are a better model than the tendon insertions of the rat for the investigation of calcified fibrocartilage in elderly hip fractures.

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“…The shape of the curve can be affected by topography, probe current, scan rate, and other factors, particularly at the margins of the histogram (Howell and Boyde, 1994;. Despite these variations, the weighted mean gray-level (WMGL) of a histogram has shown a strong linear correlation to the percent ash of bone (Bloebaum et al, , 1997Roschger et al, 1998). Ash percent in turn correlates with mechanical properties of bone (Currey et al, 1979).…”

Section: Methodsmentioning

confidence: 99%

Age‐Related mineralization heterogeneity changes in trabecular bone of the proximal femur

et al. 2004

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Although there is extensive documentation in the literature regarding the importance of trabecular bone for proximal femoral integrity and fracture resistance, there remain gaps in our understanding of the basic mineral changes that may occur in trabecular bone attributable to aging. It is unclear what age-related changes take place in the trabecular bone of the proximal femur, a common fracture site in the elderly. It has been suggested that some explanation for conflicting reports on cancellous bone may be found at a microscopic level. The goal of this study was to document age-related changes in micromineralization in the proximal femur of Caucasian females using backscattered electron imaging technology. Proximal femurs were obtained from 11 young and 11 elderly females. Sections of bone from the superior and inferior neck and superior and inferior trochanter were analyzed in a scanning electron microscope using the backscatter technique to determine ash percent. Mean ash percent did not change with age in any of the four regions (P Ͼ 0.05). However, while the mean ash percent did not change, there was a dramatic increase in variability elderly age group and loss of mineral heterogeneity. This indicates that there are subpopulations with higher or lower ash percents than the mean in the elderly study group in this investigation. While variance changed dramatically, variance within individuals did not change significantly with age (P Ͼ 0.05). The results of this study suggest that changes in micromineralization may occur within an individual, adding a possible new dimension to our understanding of fracture risk in the elderly. Future studies should examine a longer population base to confirm this observation. Published 2004 Wiley-Liss, Inc. †

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Determining mineral content variations in bone using backscattered electron imaging

Cited by 151 publications

References 30 publications

Calcium phosphate drug delivery system: influence of local zoledronate release on bone implant osteointegration

Calcium phosphate drug delivery system: influence of local zoledronate release on bone implant osteointegration

Experimental confirmation of the sheep model for studying the role of calcified fibrocartilage in hip fractures and tendon attachments

Age‐Related mineralization heterogeneity changes in trabecular bone of the proximal femur

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