Determination of β-Carotene in Supplements and Raw Materials by Reversed-Phase High Pressure Liquid Chromatography: Collaborative Study

Szpylka, John; DeVries, Jonathan W

doi:10.1093/jaoac/88.5.1279

Cited by 25 publications

(4 citation statements)

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“…A reported HPLC approach's linearity range and accuracy were 0.20-35 µg g −1 and 97.94-101.02%, respectively, which were somewhat less than the greener reversed-phase HPTLC approach (linearity range = 25-1000 ng band −1 and accuracy = 99.41-101.23%) [18]. Another HPLC approach's linearity range, accuracy, and precision were reported as 0.10-50 µg mL −1 , 97.50-102.10%, and 1.20-4.40% respectively, all of which were inferior to the greener reversed-phase HPTLC approach [23]. The reported HPTLC approach has a linearity range of 0.76-9.14 µg band −1 , which was significantly inferior than the greener reversed-phase HPTLC approach [31].…”

Section: Comparison With Literature Analytical Approachesmentioning

confidence: 96%

“…Fewer ultraviolet (UV)-based spectrometry approaches are utilized for β-carotene estimation either alone or in combination with other phytopharmaceuticals in various vegetable crops [18,19]. Various "high-performance liquid chromatography (HPLC)" approaches are also used to determine β-carotene either alone or in combination with other phytopharmaceuticals in various vegetable crops, nutritional supplements, and commercial formulations [20][21][22][23][24][25][26][27][28]. Several "high-performance thin-layer chromatography (HPTLC)" approaches are also used to determine β-carotene either alone or in combination with other phytopharmaceuticals in various vegetable crops, nutritional supplements, and commercial formulations [29][30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

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A Greener HPTLC Approach for the Determination of β-Carotene in Traditional and Ultrasound-Based Extracts of Different Fractions of Daucus carota (L.), Ipomea batatas (L.), and Commercial Formulation

Alqarni

Alam

et al. 2021

Agronomy

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Various analytical approaches for determining β-carotene in vegetable crops and commercial dosage forms have been documented. However, neither the qualitative nor quantitative environmental safety and greener aspects of the literature analytical methodologies of β-carotene analysis have been assessed. As a result, the goal of this research is to develop and validate a reversed-phase “high-performance thin-layer chromatography (HPTLC)” approach for determining β-carotene in traditional (TE) and ultrasound-assisted (UBE) extracts of different fractions of Daucus carota (L.), Ipomea batatas (L.), and commercial formulation. The greener mobile phase for β-carotene analysis was a ternary mixture of ethanol, cyclohexane, and ammonia (95:2.5:2.5, v v v−1). The detection of β-carotene was done at a wavelength of 459 nm. In the 25–1000 ng band−1 range, the greener reversed-phase HPTLC approach was linear. Other validation factors for β-carotene analysis, including as accuracy, precision, robustness, and sensitivity, were likewise dependable. The contents of β-carotene were found to be maximum in hexane: acetone (50:50%) fractions of TE and UBE of D. carota and I. batatas compared to their acetone and hexane fractions. The amount of β-carotene in hexane: acetone (50:50%) portions of TE of D. carota, I. batatas and commercial formulation A was estimated to be 10.32, 3.73, and 6.73 percent w w−1, respectively. However, the amount of β-carotene in hexane: acetone (50:50%) portions of UBE of D. carota, I. batatas and commercial formulation A was estimated to be 11.03, 4.43, and 6.89 percent w w−1, respectively. The greenness scale for the proposed HPTLC strategy was calculated as 0.81 using the “analytical GREEnness (AGREE)” method, indicating that the proposed HPTLC methodology has good greenness. The UBE approach for extracting β-carotene outperformed the TE procedure. These results indicated that the greener reversed-phase HPTLC approach can be utilized for the determination of β-carotene in different vegetable crops, plant-based phytopharmaceuticals, and commercial products. In addition, this approach is also safe and sustainable due to the utilization of a greener mobile phase compared to the toxic mobile phases utilized in literature analytical approaches of β-carotene estimation.

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Section: Comparison With Literature Analytical Approachesmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

A Greener HPTLC Approach for the Determination of β-Carotene in Traditional and Ultrasound-Based Extracts of Different Fractions of Daucus carota (L.), Ipomea batatas (L.), and Commercial Formulation

Alqarni

Alam

et al. 2021

Agronomy

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“…Szpylka & De Vries, 2005; Blake, 2007). Some standardization in methodology for the analysis of retinol and β-carotene in foods has been undertaken (20, 21). Although hampered by the large range of food matrices, official methods have been published for quantifying retinol and β-carotene in foods, which include information on foods included in the validation (22–25).…”

Section: Vitamin Amentioning

confidence: 99%

Challenges to Quantify Total Vitamin Activity: How to Combine the Contribution of Diverse Vitamers?

Jakobsen

Melse‐Boonstra

Rychlik

2019

Current Developments in Nutrition

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This state-of-the-art review aims to highlight the challenges in quantifying vitamin activity in foods that contain several vitamers of a group, using as examples the fat-soluble vitamins A and D as well as the water-soluble folate. The absorption, metabolism, and physiology of these examples are described along with the current analytical methodology, with an emphasis on approaches to standardization. Moreover, the major food sources for the vitamins are numerated. The article focuses particularly on outlining the so-called SLAMENGHI factors influencing a vitamer's’ ability to act as a vitamin, that is, molecular species, linkage, amount, matrix, effectors of absorption, nutrition status, genetics, host-related factors, and the interaction of these. After summarizing the current approaches to estimating the total content of each vitamin group, the review concludes by outlining the research gaps and future perspectives in vitamin analysis. There are no standardized methods for the quantification of the vitamers of vitamin A, vitamin D, and folate in foods. For folate and β-carotene, a difference in vitamer activity between foods and supplements has been confirmed, whereas no difference has been observed for vitamin D. For differences in vitamer activity between provitamin A carotenoids and retinol, and between 25-hydroxyvitamin D and vitamin D, international consensus is lacking. The challenges facing each of the specific vitamin communities are the gaps in knowledge about bioaccessibility and bioavailability for each of the various vitamers. The differences between the vitamins make it difficult to formulate a common strategy for assessing the quantitative differences between the vitamers. In the future, optimized stationary digestive models and the more advanced dynamic digestive models combined with in vitro models for bioavailability could more closely resemble in vivo results. New knowledge will enable us to transfer nutrient recommendations into improved dietary advice to increase public health throughout the human life cycle.

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“…The calibration standards used were pure compounds from Sigma, purity > 98%. The purity of the standards for each calibration was determined by a series of spectrophotometric measurements (UV 340/455/483 nm) method described in DIN EN 12823-2:2000 [28][29][30].…”

Section: Physico-chemical Propertiesmentioning

confidence: 99%

Impact of drying methods on the nutrient profile of fruits ofCordia africanaLam. in Tigray, northern Ethiopia

Tewolde‐Berhan¹,

Remberg²,

Abegaz³

et al. 2015

Fruits

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Introduction. Cordia africana Lam. is a tree that produces small fruits eaten in Ethiopia and other parts of Africa. The fresh fruit are sold in the market place in open bowls and, traditionally, the fruit is dried on the tree like dates. Materials and methods. Modern methods of processing the fruit were studied. Some of the changes in the nutrient content and chemistry of the fruit were measured using standard methods. Results and discussion. In the fresh fruit, the traditional method of handling collected 7.6 g dust kg −1. The TP (total phenols measured with Folin Ciocalteu's reagent) contents varied significantly between the processing methods. Using a solar dryer, the fruit dried within 5 days while the on-tree drying process took 63 days. The taste of the fruit from the direct solar dryer was less preferred to those dried on the tree. The TP values were found to be good in the dried fruits with 200 g fruit being enough to meet the daily requirement. In addition, the vitamin A and vitamin C levels were still good after drying and storage. For both fresh and dried fruits the organic acid and basic sugar profile did not show a very clear picture, as the difference in rainfall and evaporation rates between the two years of the study influenced these parameters. The headspace gas chromatography (HSGC) gave 20 volatile organic compounds and the coupled mass spectrometry (HSGC-MS) gave 39 volatile organic compounds. Conclusion. The dried fruit of Cordia africana could be used to partially meet the daily nutritional requirements of households in Ethiopia.

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Determination of β-Carotene in Supplements and Raw Materials by Reversed-Phase High Pressure Liquid Chromatography: Collaborative Study

Cited by 25 publications

References 0 publications

A Greener HPTLC Approach for the Determination of β-Carotene in Traditional and Ultrasound-Based Extracts of Different Fractions of Daucus carota (L.), Ipomea batatas (L.), and Commercial Formulation

A Greener HPTLC Approach for the Determination of β-Carotene in Traditional and Ultrasound-Based Extracts of Different Fractions of Daucus carota (L.), Ipomea batatas (L.), and Commercial Formulation

Challenges to Quantify Total Vitamin Activity: How to Combine the Contribution of Diverse Vitamers?

Impact of drying methods on the nutrient profile of fruits ofCordia africanaLam. in Tigray, northern Ethiopia

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